A5102958095- Pancreatic function and diabetes
- Molecular Biology Techniques and Applications
- Environmental DNA in Biodiversity Studies
- Diet and metabolism studies
- Diabetes and associated disorders
- Forensic and Genetic Research
- Electroconvulsive Therapy Studies
- Antioxidant Activity and Oxidative Stress
- Pluripotent Stem Cells Research
- Asthma and respiratory diseases
- Infant Nutrition and Health
- Allergic Rhinitis and Sensitization
- Electrolyte and hormonal disorders
- Birth, Development, and Health
- Neurogenesis and neuroplasticity mechanisms
- Liver Disease Diagnosis and Treatment
- Adipose Tissue and Metabolism
- CRISPR and Genetic Engineering
- Fatty Acid Research and Health
- Transcranial Magnetic Stimulation Studies
- Diet, Metabolism, and Disease
- Respiratory viral infections research
- Respiratory and Cough-Related Research
- Bacillus and Francisella bacterial research
- Pneumonia and Respiratory Infections
Lund University
2013-2024
Swedish Police Authority
2019-2024
Swedish National Forensic Centre
2018-2019
AstraZeneca (Sweden)
2004-2011
International Neuromodulation Society
2008-2011
Wallenberg Wood Science Center
2005-2009
Tampere University
2008
Tampere University Hospital
2008
Uppsala University
1993-2007
British Nuclear Medicine Society
2006
Abstract Fibrotic scar tissue limits central nervous system regeneration in adult mammals. The extent of fibrotic generation and distribution stromal cells across different lesions the brain spinal cord has not been systematically investigated mice humans. Furthermore, it is unknown whether scar-forming have same origin throughout types lesions. In current study, we compared scarring human pathological corresponding mouse models penetrating non-penetrating injury, traumatic ischemic stroke,...
Real-time polymerase chain reaction (qPCR) is the cornerstone of DNA analysis, enabling detection and quantification minute nucleic acid amounts. However, PCR-based analysis limited, in part, by presence inhibitors samples. PCR inhibition has been viewed solely as failure to efficiently generate amplicons, that is, amplification inhibition. Humic substances (HS) are well-known amplification. Here we show HS from environmental samples, specifically humic (HA), very potent inhibitors, quench...
Efficient microbial sampling from surfaces for subsequent detection and quantification is crucial in fields such as food safety hygiene monitoring. Cotton swabs are traditionally used sample collection, but today there numerous of alternative material different sizes available. Recovery efficiencies applications have been compared several studies. However, the results often contradictory. We 15 made cotton (n = 5), flocked nylon 3) foam 7), Listeria monocytogenes mengovirus on small (4 cm2)...
Ischemic stroke, a leading cause of death and disability worldwide, frequently results in cortical damage. Due to disrupted neural connectivity, lesions often trigger secondary neurodegeneration remote brain regions, such as the thalamus. This thalamic injury exacerbates neurological deficits, long-term sensory, motor, cognitive impairments. Although animal models have demonstrated that damage is driven by retrograde degeneration, excitotoxicity, apoptosis, blood-brain barrier disruption,...
ABSTRACT. The influence of food intake on the bioavailability isoniazid (INH) has been examined in nine healthy male volunteers. INH was administered as a single oral dose, both fasting state and together with standardized breakfast. Numerous venous blood samples were obtained 5 min‐6 hours after ingestion, concentrations unmetabolized serum assessed by spectrophotometry. observations indicate that peak concentration total amount absorbed are greatly reduced when drug is ingested food. Hence...
Summary Background The existence of a link between inflammation in upper and lower airways is well established. It may therefore be assumed that the nose could used to study inflammatory events airways. Objective This aimed evaluate lipopolysaccharide ( LPS ) nasal challenge model by investigating effect CXCR 2 inhibitor AZD 8309 on neutrophilic inflammation. Methods A total 18 healthy volunteers were randomized placebo‐controlled, double‐blind, cross‐over study. or placebo was dosed for 3...
Tape-lifting has since its introduction in the early 2000′s become a well-established sampling method forensic DNA analysis. Sampling is quick and straightforward while following extraction more challenging due to "stickiness", rigidity size of tape. We have developed, validated implemented simple efficient direct lysis protocol for adhesive tapes that requires limited manual labour. The uses Chelex beads applied with SceneSafe FAST This provided higher mean yields than PrepFiler Express BTA...
Despite the wide-spread use of polymerase chain reaction (PCR) in various life-science applications, causes arrested amplicon generation late cycles have not been confidently identified. This so-called plateau phase has attributed to depletion or thermal break-down primers nucleotides, inactivation DNA polymerase, and product accumulation resulting competition between primer annealing re-hybridization as well blocking by double-stranded amplicons. In current study, we experimentally...
Neuronal loss and axonal demyelination underlie long-term functional impairments in patients affected by brain disorders such as ischemic stroke. Stem cell-based approaches reconstructing remyelinating neural circuitry, leading to recovery, are highly warranted. Here, we demonstrate the vitro vivo production of myelinating oligodendrocytes from a human induced pluripotent stem cell (iPSC)-derived neuroepithelial (lt-NES) line, which also gives rise neurons with capacity integrate into...
Massively parallel sequencing (MPS) is increasingly applied in forensic short tandem repeat (STR) analysis. The presence of stutter artefacts and other PCR or errors the MPS-STR data partly limits detection low DNA amounts, e.g., complex mixtures. Unique molecular identifiers (UMIs) have been several scientific fields to reduce noise sequencing. UMIs consist a stretch random nucleotides, unique barcode for each starting molecule, that incorporated template using either ligation PCR. used...
Summary Background Intermittent allergic rhinitis (IAR) results from interactions between a large number of pro‐ and anti‐inflammatory mediators. Little is known about mediators in IAR. DNA microarrays allow simultaneous analysis the whole transcriptome sample. Objective To identify transcripts nasal fluid cells patients with IAR during season healthy controls. Methods Nasal lavage fluids were obtained 15 symptomatic birch/and or grass pollen‐induced 28 RNA was extracted pooled into one...
Pulsatile changes in the volume of an arm segment were recorded with air-filled plethysmograph and related to intra-arterial blood pressure. Alterations transmural arterial pressure obtained by changing a large chamber surrounding entire arm. Arterial compliance values calculated late diastole when slow. Compliance varied hyperbolic manner, rising steeply at low Noradrenaline hypertensin did not change values, while dihydroergotamine reduced them. Nitroglycerine caused pronounced increase...
In recent years, more sophisticated DNA technologies for genotyping have enabled considerable progress in various fields such as clinical genetics, archaeogenetics and forensic genetics. samples previously rejected too challenging to analyze due low amounts of degraded can now provide useful information. To increase the chances success with new methodologies, it is crucial know fragment size template molecules, whether a sample mostly single or double stranded. With this knowledge, an...
Bronchiolitis is a major cause of hospitalization during infancy. The aim this study was to evaluate the influence socioeconomic factors on infants with bronchiolitis. All below 1 y age living in city Malmö, Sweden, and hospitalized at University Hospital, owing bronchiolitis years 1998 1999 were identified. rates (BHRs) determined for each 10 different administrative residential areas Malmö correlated socio-economic (percentage immigrants, per capita income, unemployment rate households...