A5027660444- Acute Lymphoblastic Leukemia research
- Neuroblastoma Research and Treatments
- Childhood Cancer Survivors' Quality of Life
- Hematopoietic Stem Cell Transplantation
- Hemoglobinopathies and Related Disorders
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- Blood disorders and treatments
- Lymphoma Diagnosis and Treatment
- Neutropenia and Cancer Infections
- Blood groups and transfusion
- Immunodeficiency and Autoimmune Disorders
- CAR-T cell therapy research
- Anesthesia and Sedative Agents
- Glioma Diagnosis and Treatment
- Autoimmune and Inflammatory Disorders Research
- RNA modifications and cancer
- Iron Metabolism and Disorders
- Cancer, Hypoxia, and Metabolism
- Anesthesia and Neurotoxicity Research
- Polyomavirus and related diseases
- Parvovirus B19 Infection Studies
- Renal Transplantation Outcomes and Treatments
- Vascular Malformations and Hemangiomas
- Sarcoma Diagnosis and Treatment
Phramongkutklao Hospital
2014-2025
Phramongkutklao College of Medicine
2020-2024
Palmetto Hematology Oncology
2021
St. Jude Children's Research Hospital
2012-2013
University of Tennessee Health Science Center
2013
Institute of Cell Biology
2008-2009
Hospital for Sick Children
2005-2008
SickKids Foundation
2007
University of Toronto
2007
Institute of Infection and Immunity
2006
Patients with severe thalassemia commonly have a survival that is significantly shorter than of the general population. Allogeneic hematopoietic stem cell transplantation (allo-SCT) only established treatment potentially curative, but it limited by availability donors and medical condition patient. To expand donor pool to include haploidentical related donors, we introduced program consisting pharmacologic pretransplant immune suppression phase (PTIS) 2 courses dexamethasone fludarabine,...
Killer-cell immunoglobulin-like receptors (KIRs) that regulate natural-killer cells are highly polymorphic. Some KIR2DL1 alleles encode have stronger signaling function than others. We tested the hypothesis clinical outcomes of allogeneic hematopoietic stem-cell transplantation (HSCT) could be affected by donor polymorphism.All 313 pediatric patients received HSCT at a single institution. Donor functional allele typing was retrospectively performed using nucleotide polymorphism...
Killer cell Ig-like receptors (KIRs) on NK cells have been linked to a wide spectrum of health conditions such as chronic infections, autoimmune diseases, pregnancy complications, cancers, and transplant failures. A small subset effector memory T also expresses KIRs. In this study, we use modern analytic tools including genome-wide multiplex molecular, phenotypic, functional assays characterize the KIR(+) in human blood. We find that primarily reside CD56(+) population is distinctively...
Background Acute lymphoblastic leukemia (ALL) is the most common malignancy among children. Disease-based risk stratification was incoporated in Thai Pediatric Oncology Group (ThaiPOG) protocols to adjust intensive chemotherapies,and central nervous system-directed treatment. Although treatment outcomes have improved over decade, excellent are counterbalanced by treatment-related toxicities, especially countries with limited resources like Thailand. Objective The single institute study aimed...
Improving outcomes among class 3 thalassemia patients receiving allogeneic hematopoietic stem cell transplantations (HSCT) remains a challenge. Before HSCT, who were ≥ 7 years old and had liver size 5 cm constitute what the Center for International Blood Marrow Transplant Research defined as very high–risk subset of conventional high-risk group (here referred to HR). We performed HSCT in 98 with related unrelated donor cells. Seventy-six age < 10 received more myeloablative conditioning...
Background Shwachman-Diamond syndrome is an inherited multisystem disorder characterized by bone marrow and pancreatic dysfunction as well metaphyseal dysostosis. Ninety percent of the patients have mutations in Shwachman-Bodian-Diamond gene (SBDS). The relationship between SBDS cell survival unknown. In this study we investigated whether deficiency protein can cause increased apoptosis and, if so, what pathways are involved process.Design Methods To determine accelerated cells caused a...
Donor lymphocyte infusion (DLI) is commonly used to treat leukemia relapse following stem cell transplantation. In florid relapse, however, the efficacy of DLI limited with substantial risk severe graft-versus-host disease (GvHD). Here, we develop a novel risk-adapted strategy characterized by pre-emptive initiated at time mixed chimerism, small starting dose based on donor source, dose-escalation guided real-time chimerism monitoring and withholding immediately in patients achieving full...
Shwachman-Diamond Syndrome (SDS) is a multi-system genetic disorder with bone marrow failure. SBDS, the gene associated SDS, has been postulated to play role in ribosome biogenesis and RNA processing, but its functions are still unknown. To study whether these pathways interrupted when Sbds protein lost, we studied expression of related genes patient SBDS-/- cells by an oligonucleotide microarray. We first analysed ribosomal (RP) genes, which normally co-regulated. In 27 85 RP were...
Patients with class 3 thalassemia high-risk features for adverse events after high-dose chemotherapy hematopoietic stem cell transplantation (HSCT) are difficult to treat, tending either suffer serious toxicity or fail establish stable graft function. We performed HSCT in 18 such patients age ≥7 years and hepatomegaly using a novel approach pretransplant immunosuppression followed by myeloablative reduced-toxicity conditioning regimen (fludarabine i.v. busulfan [Flu-IV Bu]) then HSCT. The...
Despite the use of intensive contemporary multimodal therapy, overall survival patients with high-risk neuroblastoma is still less than 50%. Therefore, immunotherapy without cross-resistance and overlapping toxicity has been proposed. In this study, we report development a novel strategy to specifically activate expand human CD56(+) (NCAM1) natural killer (NK) immune cells from normal donors neuroblastoma. Enriched peripheral blood were mixed CD56(-) fraction at 1:1 ratio cultured in...
Background: α-Thalassemia, one of the major thalassemia types in Thailand, is caused by either deletion or non-deletional mutation both α-globin genes. Inactivation three genes causes hemoglobin H (Hb H) disease, and combination Hb disease with heterozygous E E) results AE Bart's disease. Objective: This study aimed to characterize clinical hematological manifestations 76 pediatric patients diseases treated at Phramongkutklao Hospital, a tertiary care center for central Thailand. Patients...
Summary Shwachman–Diamond syndrome (SDS) is an inherited bone marrow failure disorder with cytopenia and a high propensity for myelodysplastic (MDS) leukaemia, particularly acute myeloid leukaemia. The mechanism of leukaemogenesis in SDS unknown. In accordance to the multi‐hit theory carcinogenesis, it likely that several molecular cellular hits occur before MDS/leukaemia become apparent. This study used oligonucleotide microarray identify gene expression patterns, which were shown be...
High-dose methotrexate (HD-MTX) is widely used as a standard chemotherapeutic agent in pediatric cancers. Most research studies have confirmed the therapeutic efficacy of HD-MTX; however, strategies to prevent side effects vary among institutions, especially developing countries, with limited monitoring plasma level.To evaluate effect intravenous hydration during HD-MTX administration on clearance oncology patients.This study retrospectively reviewed 165 courses administered children acute...
Introduction: Beta-thalassemia is a group of inherited hemolytic anemias and one the most common genetic disorders in Thailand. The clinical spectrum beta-thalassemia disease ranges from mild to severe symptoms including intermedia (TI) major (TM). Objective: This study aimed determine correlation between beta-globin gene ( HBB ) mutations their phenotypic manifestations by evaluating patients' characteristics, transfusion requirements, growth hematologic parameters, hemoglobin typing among...
This study aimed to enhance the learning experience among medical students by empowering them co-create tools and classroom activities.
Abstract Background Neuroblastoma is the most common extra-cranial solid tumor among children. Despite intensive treatment, patients with advanced disease mostly experience dismal outcomes. Here, we proposed use of topotecan and cyclophosphamide containing induction regimen as an upfront therapy to high risk neuroblastoma patients. Methods Patients undergoing ThaiPOG protocol from 2016 2017 were studied. All received 6 cycles consisting 2 (1.2 mg/m /day) (400 for 5 days followed by cisplatin...
Background: The most common complication among pediatric oncology patients is febrile neutropenia (FN). Invasive fungal disease (IFD) suspected when fever persists > 4– 7 days after empirical antibiotics. Its clinical characteristics and predictive factors associated with IFD FN were thus explored. Methods: Pediatric between January 1, 2012 December 31, 2016 enrolled in this study. Clinical characteristics, including laboratory investigations, treatment modalities, final outcomes of...
Background . Thalassemia is a common congenital hemolytic disorder. In severe cases, regular blood transfusion essentially required. The role of premedications to prevent reactions varied among institutions with no standard guideline. Objective To prospectively compare the risk in thalassemia patients premedicated acetaminophen and chlorpheniramine maleate (CPM) versus placebo prior transfusion. Material Method A randomized, double-blinded, placebo-controlled reaction study 147 eligible was...