A5101913220- Hepatitis C virus research
- Pain Mechanisms and Treatments
- Hepatitis B Virus Studies
- Catalysis and Hydrodesulfurization Studies
- Analytical chemistry methods development
- Air Quality and Health Impacts
- Healthcare and Venom Research
- Acupuncture Treatment Research Studies
- Traumatic Brain Injury and Neurovascular Disturbances
- MicroRNA in disease regulation
- RNA modifications and cancer
- Thermal Regulation in Medicine
- Analytical Chemistry and Chromatography
- Sunflower and Safflower Cultivation
- Biodiesel Production and Applications
- Neurological Disease Mechanisms and Treatments
- Natural Antidiabetic Agents Studies
- Nerve injury and regeneration
- Epigenetics and DNA Methylation
- HIV/AIDS Research and Interventions
- Thyroid Disorders and Treatments
- Hepatitis Viruses Studies and Epidemiology
- Liver Disease Diagnosis and Treatment
- Thermochemical Biomass Conversion Processes
- Neuropeptides and Animal Physiology
Zhejiang University
2012-2024
Sun Yat-sen University
2009-2024
Peking University
2000-2024
Shanghai Changzheng Hospital
2023-2024
National Health and Family Planning Commission
2024
First Affiliated Hospital Zhejiang University
2020-2023
Shanghai Clinical Research Center
2023
Zhejiang Cancer Hospital
2023
Second Military Medical University
2023
Hangzhou Hospital of Traditional Chinese Medicine
2023
CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian cell engineering. However, the delivery Cas9 and synthesis guide RNA (gRNA) remain as steps can limit overall ease use. Here we describe methods rapid gRNA protein/gRNA ribonucleoprotein complexes (Cas9 RNPs) into variety cells through liposome-mediated transfection or electroporation. Using these methods, report nuclease-mediated indel rates up to 94% in Jurkat T...
Abstract Background Anthocyanins are a group of natural products widely found in plants. They have been to alleviate the disorders glucose metabolism type 2 diabetes mellitus (T2DM), while underlying mechanisms remain unclear. Methods HepG2 and L02 cells were incubated with 0.2 mM PA 30 for 24 h induce IR, treated 5 used as control. C57BL/6 J male mice db/db fed chow diet gavaged pure water or cyanidin-3-O-glucoside (C3G) solution (150 mg/kg/day) 6 weeks. Results In this study, anthocyanin...
Novel supported task-specific ionic liquids have been developed for the first time via ionic-pair coupling of imidazolium cation modified polystyrene support with l-proline. The materials shown an efficient metal scavenging ability (e.g., CuI, Pd(OAc)2, Pd0, and IrCl3) without aid a nonimmobilized liquid, which relies on highly synergistic effect coordination nitrogen atom COO- group l-proline moiety, electrostatic forces, steric protection. resulting metal-soaked can be used as...
Backgrounds In advanced pancreatic ductal adenocarcinoma (PDAC), immune therapy, including checkpoint inhibitors, has limited efficacy, encouraging the study of combination therapy. Methods Tumor necrosis factor receptor 2 (TNFR2) was analyzed via immunohistochemistry, immunofluorescence, western blotting, and ELISAs. The in vitro mechanism that TNFR2 regulates programmed cell death 1 ligand (PD-L1) investigated using flow cytometry, chromatin immunoprecipitation (ChIP). vivo efficacy...
Angiogenesis is a major obstacle for wound healing in patients with diabetic foot wounds. Mesenchymal stem cells (MSCs) have an important function repair, and neurotrophin-3 (NT-3) can promote nerve regeneration angiogenesis. We investigated the effect of NT-3 on accelerating by improving human bone marrow MSC (hMSC) activation. In vitro, significantly promoted VEGF, NGF, BDNF secretion hMSCs. improved activation hMSC conditioned medium, umbilical vein endothelial cell (HUVEC) proliferation...
In this paper, bio-oil from fast pyrolysis of Pinus sylvestris L. was upgraded over supported noble metal catalysts in supercritical monoalcohols under a hydrogen atmosphere. Esterification, cracking (both alcoholysis and hydrolysis), hydrogenation, along with acetalization, isomerization, other reactions were combined during the upgrading process. The product analysis showed that processing ethanol Pt/SO42–/ZrO2/SBA-15 had good performance. removal acids aldehydes decrease ketones, phenols,...
Microplastics (MPs) ingested and accumulated by organisms would ultimately pose a threat to humans via the food chain. A balanced gut microbiota contributes many health benefits, which is readily influenced environmental chemicals such as MPs. Cyanidin-3-O-glucoside (C3G), bioactive compound of anthocyanin family, possesses variety functional effects including anti-oxidant anti-inflammatory, well modulation. C3G has been demonstrated prevent polystyrene (PS) induced toxicities in Caco-2...
Abstract Background White matter injury (WMI) represents a significant etiological factor contributing to neurological impairment subsequent Traumatic Brain Injury (TBI). CD36 receptors are recognized as pivotal participants in the pathogenesis of disorders, including stroke and spinal cord injury. Furthermore, dynamic fluctuations phenotypic polarization microglial cells have been intimately associated with regenerative processes within injured tissue following TBI. Nevertheless, there is...
A large proportion of patients with chronic pain experience co-morbid anxiety. The medial prefrontal cortex (mPFC) is proposed to underlie this comorbidity, but the molecular and neuronal mechanisms are not fully understood. Here, we reported that impaired macroautophagy in prelimbic cortical (PrL) subregion mPFC paralleled occurrence anxiety-like behaviors rats spared nerve injury (SNI). Intriguingly, such impairment was mainly observed a FOS/c-Fos+ subpopulation PrL. Chemogenetic...
Abstract Sorafenib (SOR) resistance remains a major obstacle in the effective treatment of hepatocellular carcinoma (HCC). A number long noncoding RNAs (lncRNAs) are responsible for this chemoresistance. This study aimed to reveal essential function recently defined lncRNA, lncRNA‐POIR, epithelial–mesenchymal transition (EMT) and SOR sensitivity HCC cells. SOR‐induced cytotoxicity was analyzed via cell counting kit‐8 ethynyl‐2'‐deoxyuridine incorporation assays, whereas immunoblotting...
Cyanidin-3-O-glucoside (C3G), an essential representative of anthocyanins, has been proved to possess a myriad biological activities. However, the effects C3G on glucose metabolism and its underlying molecular mechanisms remain elusive. The aim present study was investigate metabolic impact db/db mice determine whether consequent anti-diabetic were related transporter-1 (GLUT-1) by in vivo vitro studies. As result, through diabetic mice, treatment found significantly reduce fasting blood...
While a few works have shown that Mettl3 plays oncogenic roles in hepatocellular carcinoma (HCC), its function early HCC tumorigenesis remains unclear. In Mettl3flox/flox; Alb-Cre knockout mice, loss leads to aberrant hepatocyte homeostasis and liver damage. Importantly, deletion dramatically accelerates various mouse models. Depletion of adult Mettl3flox/flox mice through TBG-Cre administration also enhances tumor development, while overexpression inhibits hepatocarcinogenesis....
Background: Intrahepatic cholangiocarcinoma (iCCA) is an aggressive malignancy with multiple etiologies and largely refractory to current treatment strategies. Myeloid leukemia factor 1 (MLF1) associated human cancer progression. Nevertheless, the function of MLF1 in iCCA remains unknown. Methods: We performed expression analyses iCCA. In vitro vivo experiments were conducted investigate role The upstream regulatory mechanism upregulation was deciphered by luciferase DNA methylation...
Ion channels are very important in the peripheral sensitization neuropathic pain. Our present study aims to investigate possible contribution of Ca V 3.2 T-type calcium damaged dorsal root ganglion neurons We established a pain model rats with spared nerve injury. In these rats, it was easy distinguish (of tibial and common peroneal nerve) from intact sural nerves). results showed that protein expression increased medium-sized ganglions but not ones. With whole cell patch clamp recording...
Background Streptococcus pneumoniae is an important pathogen causing high morbidity and mortality in children undergoes frequent recombination for capsule switching to neutralize the 13-valent pneumococcal conjugate vaccine (PCV13). This study aimed investigate prevalence, molecular characteristics including serotypes antibiotic susceptibility of S. isolated from living Southwest China 2017 2019 facilitate selection effective formulations appropriate treatment regimens. Methods was conducted...
Abstract Background Identification of novel biomarker is important for development molecular-targeted therapy agents patients with hepatocellular carcinoma (HCC). This study aims to identify potential prognostic biomarkers and investigate epigenetic mechanism HCC development. Methods Public bulk-RNA seq datasets proteomic dataset were screened identification patients. methylomic analyzed deciphering the regulating HCC-associated gene expression. Immunoblotting, immunohistochemistry,...