A5100429137- Cancer-related molecular mechanisms research
- Drug Transport and Resistance Mechanisms
- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- RNA modifications and cancer
- Metabolism, Diabetes, and Cancer
- Protein Tyrosine Phosphatases
- Alzheimer's disease research and treatments
- Atherosclerosis and Cardiovascular Diseases
- Circular RNAs in diseases
- Drug-Induced Hepatotoxicity and Protection
- RNA Research and Splicing
- Tryptophan and brain disorders
- Diabetes Treatment and Management
- Natural product bioactivities and synthesis
- Cholesterol and Lipid Metabolism
- Autophagy in Disease and Therapy
- Pancreatic function and diabetes
- Cancer, Lipids, and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Reproductive System and Pregnancy
- Bioactive Compounds and Antitumor Agents
- Mitochondrial Function and Pathology
- Sphingolipid Metabolism and Signaling
- Peroxisome Proliferator-Activated Receptors
Soochow University
2025
The First People's Hospital of Changzhou
2025
Agriculture Fisheries and Conservation Department
2024
South China Agricultural University
2022-2024
Shanghai University of Traditional Chinese Medicine
2014-2024
Shaanxi Institute of Zoology
2024
Chengdu University
2024
Fujian Medical University
2024
Education Department of Fujian Province
2024
Xiamen University
2024
Abstract N 6 -methyladenosine (m A) on chromosome-associated regulatory RNAs (carRNAs), including repeat RNAs, plays important roles in tuning the chromatin state and transcription, but intrinsic mechanism remains unclear. Here, we report that YTHDC1 indispensable self-renewal differentiation potency of mouse embryonic stem cells (ESCs), which highly depends m A-binding ability. Ythdc1 is required for sufficient rRNA synthesis repression 2-cell (2C) transcriptional program ESCs,...
Abstract Primary ovarian insufficiency (POI) is a clinical syndrome of dysfunction characterized by premature exhaustion primordial follicles. POI causes infertility, severe daily life disturbances and long-term health risks. However, the underlying mechanism remains largely unknown. We previously identified Basonuclin 1 ( BNC1 ) mutation from large Chinese pedigree found that mice with targeted Bnc1 exhibit symptoms POI. In this study, we plays key roles in reserve maintaining lipid...
The incidence of premature ovarian failure (POF), also known as insufficiency, has been increasing in recent years. Although some treatments are currently available, improved treatment strategies urgently required. Many researchers have reported that human endometrial stem cells (HuMenSCs), which exhibit stem/progenitor cell properties vitro repaired damaged vivo. Thus, we aimed to determine whether HuMenSCs can serve therapy tools and be used for the POF. After treating with...
The aim of this study was to investigate the mechanisms through which quercetin protects against atherosclerosis (AS) in apoE‑/‑ mice by regulating expression proprotein convertase subtilisin/kexin type 9 (PCSK9), cluster differentiation 36 (CD36), peroxisome proliferator‑activated receptor γ (PPARγ), liver X α (LXRα) and ATP binding cassette transporter A1 (ABCA1). We established an animal model high‑fat diet induced AS using mice. H&E, Oil Red O Masson's trichrome staining were performed...
Concentrative nucleoside transporters (Cnts) and equilibrative (Ents) have essential physiological functions are important in disposition of anticancer antiviral analogs. Information on tissue distribution Cnts Ents rodents is sparse. Thus, the present study aimed to determine Cnt1-3 Ent1-3 transcripts 19 tissues Sprague-Dawley rats C57BL/6 mice both genders. These six were quantified using branched DNA signal amplification assay. Cnt1 highest small intestine, followed by kidney testes, with...
Premature ovarian failure (POF) is a common cause of female infertility, for which there are currently no ideal treatments or medications. Furthermore, apoptosis granulosa cells (OGCs) an important mechanism underlying the decline in reserve and function. In present study, several cellular growth factors hormones were used to induce differentiation human induced pluripotent stem (iPSCs) into granulosa‑like (OGLCs) vitro. Immunohistochemical staining demonstrated that OGLCs derived from iPSCs...
As a systemic syndrome characterized by age-associated degenerative skeletal muscle atrophy, sarcopenia leads to risk of adverse outcomes in the elderly. Age-related iron accumulation is found muscles animal models and patients, but role remains poorly understood. It has been recently that overload several diseases involved ferroptosis, an iron- dependent form programmed cell death. However, whether this excess can result ferroptosis still unclear. In our present study, we ferric citrate...
Abstract Background In Alzheimer’s disease (AD), the neuroinflammatory response mediated by activation of senescent microglia is closely related to energy dysmetabolism. However, mechanism underlying interaction between metabolism aging and neuroinflammation remains unclear. Methods We used biochemical methods, enzyme-linked immunosorbent assay (ELISA), immunofluorescence, western blot determine effects CD38 knockdown on in Aβ1-40 injured BV2 cells. Using AD model mice, we detected enzyme...
CTCF is crucial for chromatin structure and transcription regulation in early embryonic development. However, the kinetics of occupation preimplantation embryos have remained unclear. In this study, we used CUT&RUN technology to investigate occupancy mouse Our findings revealed that begins binding genome prior zygotic activation (ZGA), with a preference CTCF-anchored loops. Although majority consistently maintained, identified specific set sites enriched mouse-specific short interspersed...
The aim of this study was to determine the role pregnane X receptor (PXR) in induction UDP-glucuronosyltransferases (UGTs) by pregnenolone-16 alpha-carbonitrile (PCN). Four- six-month-old male wild-type and PXR-null mice received control or PCN-treated (1500 ppm) diet for 21 days. On day 22, livers were taken prepare microsomes total RNA UGT activity mRNA levels, respectively. In mice, PCN treatment significantly increased activities toward bilirubin, 1-naphthol, chloramphenicol, thyroxine,...
APD334 was discovered as part of our internal effort to identify potent, centrally available, functional antagonists the S1P1 receptor for use next generation therapeutics treating multiple sclerosis (MS) and other autoimmune diseases. is a potent antagonist has favorable PK/PD profile, producing robust lymphocyte lowering at relatively low plasma concentrations in several preclinical species. This new agent efficacious mouse experimental encephalomyelitis (EAE) model MS rat collagen induced...
Alzheimer's disease (AD) is the most common form of senile dementia which characterized by abnormal amyloid beta (Aβ) accumulation and deposition in brain parenchyma cerebral capillaries, leads to blood-brain barrier (BBB) disruption. Despite great progress understanding etiology AD, underlying pathogenic mechanism BBB damage still unclear, no effective treatment has been devised. The standard Ginkgo biloba extract EGb761 widely used as a potential cognitive enhancer for AD. However,...
The present study examined the involvement of autophagy as a mechanism in protective effect quercetin (QUE) on atherosclerosis (AS) ApoE‑/‑ mice. An AS model was established by feeding mice high‑fat diet (HFD). Mice were divided into four experimental groups: model, QUE, 3‑methyladenine (3‑MA) and QUE + 3‑MA groups. Additionally, age‑matched wild‑type C57BL/6 used Control group. Autophagosomes aorta using transmission electron microscope. Aorta pathology, serum lipid accumulation collagen...
Abstract Objective To examine the role of high‐fat and high‐sugar (HFHS) diet‐induced oxidative stress, which is a risk factor for various diseases, in premature ovarian failure (POF). Materials methods Ovarian granulosa cells (OGCs) were isolated from mice cultured medium supplemented with HFHS poly (lactic‐co‐glycolic acid) (PLGA)‐cross‐linked miR‐146b‐5p nanoparticles (miR‐146@PLGA). RNA protein expression levels examined using quantitative real‐time polymerase chain reaction Western...
The kinetics of drug-receptor interactions can profoundly influence in vivo and vitro pharmacology. In vitro, the potencies slowly associating agonists may be underestimated assays capturing transient signaling events. When divergent receptor-mediated pathways are evaluated using combinations equilibrium assays, potency differences driven by erroneously interpreted as biased signaling. vivo, drugs with slow dissociation rates display prolonged physiologic effects inconsistent their...
// Xiaoyu Cai 1,* , Yuanyuan Hu Hao Tang 2 Han 1 Lijun Pang Junyue Xing Zhenyun Liu Yuhong Luo Bin Jiang Te 4 Myriam Gorospe 3 Chuan Chen and Wengong Wang Department of Biochemistry Molecular Biology, Peking University Health Science Center, Beijing, P. R. China Physiology Pathophysiology, P.R. Laboratory Genetics, National Institute on Aging, Institutes Health, Baltimore, MD, USA Shanghai Geriatric Chinese Medicine, Shanghai, * These authors have contributted equally to this work...
Premature ovarian failure (POF) refers to the presence of atrophic permanent amenorrhea in women under age 40. The pathogenesis POF remains be fully elucidated. Telocytes are a group specialized cells with small cell volume and very long cytoplasmic prolongations dichotomous branching. Previous studies have indicated that telocytes function support trachea serve as stem niches. Although it has been confirmed present numerous organs mammals, determined whether they tissues involved...