A5054451012- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Metabolomics and Mass Spectrometry Studies
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Infectious Encephalopathies and Encephalitis
- Cancer-related Molecular Pathways
- Peroxisome Proliferator-Activated Receptors
- Wnt/β-catenin signaling in development and cancer
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- DNA Repair Mechanisms
- Inflammatory mediators and NSAID effects
- S100 Proteins and Annexins
- Cell Adhesion Molecules Research
- Advanced Breast Cancer Therapies
- ATP Synthase and ATPases Research
- Autophagy in Disease and Therapy
- Biomarkers in Disease Mechanisms
- Nutrition, Genetics, and Disease
- High Altitude and Hypoxia
- Proteoglycans and glycosaminoglycans research
- Chronic Lymphocytic Leukemia Research
- Cellular Mechanics and Interactions
- Lung Cancer Treatments and Mutations
Hubei University of Medicine
2020-2023
Army Medical University
2009-2018
Xinqiao Hospital
2009-2018
The distinction between mild pathogenic mtDNA mutations and population polymorphisms can be ambiguous because both are homoplasmic, alter conserved functions, correlate with disease. One possible explanation for this ambiguity is that the same variant may have different consequences in contexts. NADH dehydrogenase subunit 1 (ND1) nucleotide 3394 T > C (Y30H) such a case. This has been associated Leber hereditary optic neuropathy it reduces complex I activity cellular respiration 7% 28% on...
Mitochondrial DNA (mtDNA) is particularly susceptible to oxidative damage and mutation due the high rate of reactive oxygen species (ROS) production limited DNA-repair capacity in mitochondrial. Previous studies demonstrated that increased mtDNA copy number for compensation damage, which was associated with cigarette smoking, has been found be lung cancer risk among heavy smokers. Given common "non-pathological" variations determine differences phosphorylation performance ROS production, an...
Chemoresistance has been a major obstacle to the effective treatment of lung cancer. Previously, we found that contactin-1 (CNTN-1) is related cisplatin resistance in adenocarcinoma. Here, aimed investigate underlying mechanism behind role CNTN-1 adenocarcinoma.EMT-associated phenotypes, including alterations cellular morphology and marker (E-cadherin, N-cadherin Vimentin) expression, were compared between A549 cells A549/DDP (a cisplatin-resistant cell line adenocarcinoma with abnormal...
Although aberrant DNA methylation has been implicated in the pathophysiology of lung cancer, role multidrug resistance (MDR) cancer remained unclear. To investigate whether certain distinct pattern is associated with acquired MDR adenocarcinoma, methylated-DNA immunoprecipitation-chromatin immunoprecipitation (MeDIP-ChIP) was utilised to compare genome-wide promoter human adenocarcinoma A549/cisplatin (A549/DDP) cells its progenitor A549 cells. The comparison identified 3617 genes...
Abstract Background In recent years, immunotherapies and targeted therapies contribute to population-level improvement in NSCLC cancer-specific survival, however, the two novel therapeutic options have mainly benefit patients containing mutated driven genes. Thus, explore other potential genes related with immunity or may provide improve survival of lung cancer without CTSF is unique human cysteine proteinases. Presently, has been detected several cell lines cancer, but its role progression...
To investigate the predictive value of acute physiology and chronic health evaluation 2 (APACHE2) score lung injury prediction (LIPS) for respiratory distress syndrome (ARDS) when combined with biomarkers this condition in patients ARDS risk factors. In total, 158 Han Chinese factors were recruited from Respiratory Emergency Intensive Care Units. The LIPS, APACHE2 score, primary diagnosis at admission, determined within 6 h PaO /FiO was on day admission. Blood collected 24 admission...
Abstract Objectives Chemoresistance induced by cisplatin has become the major impediment to lung cancer chemotherapy. This study explored potential chemoresistant genes and underlying mechanisms of chemoresistance in NSCLC. Materials methods Gene expression profile was integrated with DNA methylation screen candidate genes. Bioinformatic analysis immunohistochemistry were used analyse association a gene characteristics NSCLC patients. Recombinant lentivirus vectors utilized overexpress or...
Background LPS-binding protein (LBP) and its ligand CD14 are located upstream of the signaling pathway for LPS-induced inflammation. Blocking LBP binding might prevent In previous studies, we obtained a peptide analog (MP12) LBP/CD14 site showed that this had anti-endotoxin activity. study, used in vitro directed evolution to improve vivo Methods We error-prone PCR (ep-PCR) induced mutations C-terminus attached products T7 phages establish mutant phage display library. The positive clones...
Abstract Background Mitochondria have been shown to play vital roles during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and disease 2019 (COVID-19) development. Currently, it is unclear whether mitochondrial DNA (mtDNA) variants, which define mtDNA haplogroups determine oxidative phosphorylation performance reactive oxygen species production, are associated with COVID-19 risk. Methods A population-based case–control study was conducted compare the distribution of...
Mitochondrial DNA (mtDNA) alterations, including mtDNA copy number and 4977 bp common deletion (CD), are key indicators of irradiation-induced damage. The relationship between total body irradiation (TBI) treatment alterations in vivo, however, has not been postulated yet. aim this study is to analyze irradiated human peripheral lymphocytes from acute lymphoblastic leukemia (ALL) patients as well take them predictors for radiation toxicity.Peripheral blood were isolated 26 ALL 24 hours after...
// Chaowen Jiang 1, * , Shilong Yu Pin Qian Ruiling Guo 2 Ruijie Zhang 3 Zhi Ao 1 Qi Li Guoming Wu Yan Chen Jin Changzheng Wang Wei Yao Jiancheng Xu Guisheng Fuyun Ji Institute of Human Respiratory Disease, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China Department Diseases, 324 th Hospital People's Liberation Army (No.324 PLA), 400020, Medicine, Xiangya Central South Changsha, Hunan 410013, These authors have contributed equally to this work Correspondence...
This study aimed to investigate the mechanism by which human lung cancer drug resistance-related gene BC006151 regulates chemosensitivity down-regulating expression via antisense transfer in H446/CDDP cells. A retroviral vector containing sequence was constructed and transfected into Transfection of empty served as a negative control. The two groups cells were treated with various chemotherapeutic agents, after morphological changes cell ultrastructure compared transmission electron...
To predict the intervention targets of empagliflozin (EMPA), a specific inhibitor sodium-glucose cotransporter 2 (SGLT2), in gastric adenocarcinoma through comprehensive network pharmacology, and to validate effects molecular mechanisms EMPA cellular biology experiments.
Objective To determine the effects of nuclear factor-KB(NF-кB)on peroxisome proliferator activated receptor γ(PPARγ)expression in murine macrophage cell line Ana-1.based on investigation PPARγ expression stimulated by lipopolysaccharide(LPS).Methods Aas-1 cells were divided randomly into seven groups:control group,LPS groups(cells 0.1 mg/L LPS for 1,2,4,8 hours respectively),SN50 group(cells 50 SN50 4 hours)and NF-кB high plasmid transfeeted group.PPARγ and tumor necrosis...