The long non-coding RNA H19 plays a crucial role in solid tumor initiation and progression. However, the potential of its clinical significance acute myeloid leukemia (AML) remain largely elusive. expression was detected by qPCR, AML patients further analyzed. Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) data for were used as validation cohorts. roles cell proliferation apoptosis determined assay flow cytometry analysis. significantly increased but not associated with embedded...

Expression of the nuclear receptor peroxisome proliferator activated delta (PPARδ) in breast cancer cells is negatively associated with patient survival, but underlying mechanisms are not clear. High PPARδ protein levels rat adenocarcinomas were found to be increased growth soft agar and mice. Transgenic expression ability human cell lines migrate vitro form lung metastases also conferred grow exhausted tissue culture media survive low-glucose other endoplasmic reticulum stress conditions...

BCL2 protein inhibitor venetoclax (ABT-199) has been authorized by Food and Drug Administration for relapsed/refractory chronic lymphoid leukemia with 17p deletion. Although venetoclax/ABT-199 also caused cell death in acute myeloid (AML), whether it could be applied to clinical treatment needs further studies. Here, we revealed implication of overexpression de novo adult AML, may provide theoretical basis targeted therapy using venetoclax. expression was analyzed AML patients from public...

In terrestrial ecosystems, leaf litter is the main source of nutrients returning to soil. Understanding how decomposition responds stand age critical for improving predictions effects forest structure on nutrient availability and cycling in ecosystems. However, changes this process with remain poorly understood due complexity diversity patterns drivers among different ages. study, we examined two well-replicated sequences naturally occurring secondary forests Chinese fir (Cunninghamia...

DNA methyltransferases (DNMTs) by regulating methylation play crucial roles in the progression of hematologic malignancies, especially for acute myeloid leukemia (AML). Accumulating investigations have identified high incidence DNMT3A mutation AML, and it is correlated with poor prognosis. Although a few studies shown expression DNMTs their clinical significance results remain to be discussed. Herein, we systemically analyzed relationship clinic-pathological features prognosis AML patients....

Esophageal cancer is a highly invasive malignancy. Neoadjuvant chemoradiotherapy not only increases the rate of complete resection but also improves median survival. However, sensitive biomarker urgently needed in clinical practice. 60 esophageal squamous cell carcinoma (ESCC) patients undergoing neoadjuvant (NCRT) were enrolled at People's Hospital Affiliated to Jiangsu University. Patients grouped according tumor regression grade (TRG) criteria from College American Pathologists (CAP). The...

Stimulator of interferon response cGAMP interactor (STING) is essential for both innate and adaptive immunity. However, a comprehensive molecular characterization STING expression across hematological malignancies lacking. In this study, the pan-blood-cancer landscape related to was identified using GTEx, CCLE, Hemap, TCGA databases, potential value predicting prognosis investigated. The relationship between immune cell enrichment assessed in Hemap database. Moreover, efficacy immunotherapy...

Receptor-interacting protein kinase 3 (RIPK3) has been implicated in the pathogenesis of diverse human cancers. However, role RIPK3 acute myeloid leukemia (AML) is not fully understood, which needs further research and clarification. We first identified expression clinical prognostic value AML through a public database validated our cohort. In addition, biological function leukemic development was verified vitro experiments. Based on GEPIA database, we screened that overexpression among RIPK...

Abstract This study investigated the role of glutamine-fructose-6-phosphate transaminase 2 (GFPT2) in response epithelial ovarian cancer cells to paclitaxel, a standard chemotherapy drug. We analyzed GFPT2 expression across various EOC cell lines, including SKOV3, HEY, ES-2, A2780, and OVCR3. In HEY we performed knockdown, while A2780 were engineered for overexpression. Following these manipulations, assessed cellular responses paclitaxel treatment. Results demonstrated correlation between...

Abstract The deregulated DLX gene family members DLX1/2/3/4/5/6 ( DLXs ) caused by DNA methylation has been demonstrated in various cancers with therapeutic target value. However, the potential role of myeloid neoplasms such as acute leukemia (AML) and myelodysplastic syndromes (MDS) remains to be elucidated. Clinical significance methylation/expression was analyzed patient AML MDS. functional roles were determined vitro. In identification stage, we found that lower DLX5 expression...

Malocclusion is an important risk factor for temporomandibular disorder (TMD), a series of disorders characterized by dysfunction in the orofacial region involving joint (TMJ) and jaw muscles. We recently showed that experimental unilateral anterior crossbite (UAC) produced masseter hyperactivity through circuit periodontal proprioception, trigeminal mesencephalic nucleus (Vme), motor (Vmo). Anxiety common complication patients with TMD. The lateral habenula (LHb) involved emotional...

MircoRNA-335 (miR-335) has been reported as a significant cancer-associated microRNA, which was often epigenetically silenced and acted tumor suppressor gene in diverse human solid tumors. Conversely, recent studies show that miR-335 overexpression identified both adult pediatric acute myeloid leukemia (AML), suggesting it might play an oncogenic role of AML. However, the during leukemogenesis remains to be elucidated. MiR-335/ID4 expression detected by real-time quantitative PCR and/or...

Acute myeloid leukemia (AML) is a heterogeneous disease related to broad spectrum of molecular alterations. The successes immunotherapies treating solid tumors and deeper understanding the immune systems patients with hematologic malignancies have promoted development for treatment AML. And high tumor mutational burden (TMB) an emerging predictive biomarker response immunotherapy. However, association gene mutation in AML TMB immunological features still has not been clearly elucidated. In...

Although a previous pan-cancer study has reported the expression patterns of ITIHs in various tumors, their analyses have been restricted to limited cancer types. We thus comprehensively analyzed profiles and clinical significances broader spectrum cancers from TCGA. Our results showed that were primarily down-regulated tested cancers. The ITIH members associated with either survival advantage or disadvantage, depending on type genes queried. Importantly, we for first time demonstrated ITIH1...

Abstract RNA methylation plays a key role across biological processes, which could be utilized as new weapons for cancer management. However, the implication of regulators in cancers, especially clear cell renal (ccRCC), remains largely unknown. We investigated multiomics profile at pan‐cancer level. found most were dysregulated might explained by genomic mutation and copy number variation. A novel subtype ccRCC, modification 2 (RMCS2), was identified verified among different ccRCC cohorts....

Previous study has revealed that H19 expression is required for efficient tumor growth induced by BCR-ABL in chronic myeloid leukemia (CML). Herein, we further determined and its clinical implication patients with CML. methylation were detected real-time quantitative PCR methylation-specific PCR, then of was analyzed. significantly up-regulated CML (p < 0.001). an area under receiver operating characteristic curve value 0.824 might serve as a promising biomarker distinguishing from controls....

Leukemia stem cell (LSC)-enriched genes have been shown to be highly prognostic in acute myeloid leukemia (AML). However, the value of tumor suppressor (TSGs) that are repressed early LSC remains largely unknown. We compared public available expression/methylation profiling data LSCs with hematopoietic cells (HSCs), order identify potential LSC. The relevance PCDH17 was analyzed on a cohort 173 AML patients from Cancer Genome Atlas (TCGA), and further validated three independent cohorts (n =...

Abstract The potential mechanism of myelodysplastic syndromes (MDS) progressing to acute myeloid leukemia (AML) remains poorly elucidated. It has been proved that epigenetic alterations play crucial roles in the pathogenesis cancer progression including MDS. However, fewer studies explored whole-genome methylation during MDS progression. Reduced representation bisulfite sequencing was conducted four paired MDS/secondary AML (MDS/sAML) patients and intended explore underlying...

TGFB1, which encodes TGF-β1, a potent cytokine regulating varies cellular processes including immune responses. TGF-β1 plays context-dependent roles in cancers and is increasingly recognized as therapeutic target to enhance immunotherapy We comprehensively evaluated expression of TGFB1 its clinical biological effects across hematological malignancies. was first explored using data from the GTEx, CCLE, TCGA databases. The significances malignancies were analyzed Hemap our In Silico curated...

Previous studies have been indicated that integrin α2 (ITGA2) may be important in cell migration, invasion, survival, and angiogenesis. However, the correlation between ITGA2 expression acute myeloid leukemia (AML) is still unclear. Real‐time quantitative polymerase chain reaction was carried out to analyze messenger RNA level. Methylation‐specific (PCR) bisulfite sequencing PCR were performed detect methylation of promoter. significantly upregulated 134 de novo AML patients compared with 33...