A5102017174- Autophagy in Disease and Therapy
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Cell death mechanisms and regulation
- Ubiquitin and proteasome pathways
- ATP Synthase and ATPases Research
- Cancer-related Molecular Pathways
- Sirtuins and Resveratrol in Medicine
- MicroRNA in disease regulation
- Trace Elements in Health
- Peroxisome Proliferator-Activated Receptors
- Cancer-related molecular mechanisms research
- Adenosine and Purinergic Signaling
- Adipose Tissue and Metabolism
- Genomics, phytochemicals, and oxidative stress
- Metabolism and Genetic Disorders
- Cancer-related gene regulation
- Cancer Research and Treatments
- Metabolism, Diabetes, and Cancer
- RNA Research and Splicing
- DNA Repair Mechanisms
- Neurological Disease Mechanisms and Treatments
- Drug Transport and Resistance Mechanisms
Jilin University
2016-2025
Jilin Medical University
2003-2022
College of Medical Sciences
2020
Sunnybrook Hospital
2016-2017
Sunnybrook Research Institute
2016-2017
Union Hospital
2017
First Bethune Hospital of Jilin University
2008-2013
University of the Ryukyus
2000-2003
Accumulation of reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) is an oxidative stress response, which induced various defense mechanisms or programmed cell death (PCD). As one the major types PCD, autophagy has been observed in response to several anticancer drugs and demonstrated be responsible for death. To date, however, exact mechanism by ROS regulates still poorly understood. Thus, purposes this study were elucidate how H2O2 exerts its cytotoxic effects on malignant...
This report describes an integrated study on identification of potential markers for gastric cancer in patients' tissues and sera based on: (i) genome-scale transcriptomic analyses 80 paired cancer/reference (ii) computational prediction blood-secretory proteins supported by experimental validation. Our findings show that: 715 150 genes exhibit significantly differential expressions all cancers early-stage versus reference tissues, respectively; a substantial percentage the alteration is...
Neurodegenerative diseases are disorders that characterized by a progressive decline of motor and/or cognitive functions caused the selective degeneration and loss neurons within central nervous system. The most common neurodegenerative Alzheimer's disease (AD), Parkinson's (PD), Huntington's (HD). Neurons have high energy demands, dysregulation mitochondrial quality function is an important cause neuronal degeneration. Mitochondrial control plays role in maintaining integrity ensuring...
In this study, we used MTT assays to demonstrate that a combination of SPIO-Serum and wild-type p53 overexpression can reduce ovarian cancer cell viability in vitro. Prussian blue staining iron were determine changes intracellular concentration following treatment. TEM was evaluate any mitochondrial damage induced by treatment, Western blot the expression transporter lipid peroxidation regulator proteins. JC-1 measure membrane potential, ROS levels estimated flow cytometry. Finally, xCT...
The tumor cells have some metabolic characteristics of the original tissues, and metabolism is closely related to autophagy. However, mechanism autophagy in chemotherapeutic drug resistance still poorly understood. In this study, we investigated role glucose by using cholangiocarcinoma QBC939 with primary cisplatin hepatocellular carcinoma HepG2 cells. We found that had a higher capacity for uptake, consumption, lactic acid generation, activity pentose phosphate pathway compared cells, PPP...
Expression of the nuclear receptor peroxisome proliferator activated delta (PPARδ) in breast cancer cells is negatively associated with patient survival, but underlying mechanisms are not clear. High PPARδ protein levels rat adenocarcinomas were found to be increased growth soft agar and mice. Transgenic expression ability human cell lines migrate vitro form lung metastases also conferred grow exhausted tissue culture media survive low-glucose other endoplasmic reticulum stress conditions...
Diabetes mellitus is associated with cognitive dysfunction. Numerous previous studies have shown that type 1 diabetes-induced hyperglycaemia causes structural brain damage, such as a decrease in whole-brain grey matter. The impact of diabetes on the cerebral cortex poorly understood and requires further clarification. In present study, was induced via an intraperitoneal injection streptozotocin (50 mg/kg). Hematoxylin eosin (H&E) staining performed to detect morphological changes cortex,...
The poor prognosis and high mortality of patients with ovarian cancer result in part from their response to platinum-based chemotherapy. However, the precise mechanism behind cisplatin resistance is still not fully understood. In present study, authors explored perspective glucose metabolism human cancer. experiments using genetically matched cell lines SKOV3 (cisplatin-sensitive) SKOV3/DDP (cisplatin-resistant) study provided some important findings. First, comparison cells, cells exhibited...
Pyruvate dehydrogenase kinase 1 (PDK1) is overexpressed in ovarian cancer and thus a promising anticancer therapeutic target. Our previous work suggests that coumarin compounds are potential inhibitors of PDKs. In this study, we used the cell line SKOV3 as model system examined whether dicumarol (DIC), compound, could inhibit through targeting PDK1. We showed DIC potently inhibited activity PDK1, shifted glucose metabolism from aerobic glycolysis to oxidative phosphorylation, generated...
Platinum‐based therapeutic strategies have been widely used in ovarian cancer treatment. However, drug resistance has greatly limited efficacy. Recently, tolerance to cisplatin attributed other factors unrelated DNA . p62 (also known as SQSTM 1) functions a multifunctional hub participating tumorigenesis and may be target. Our previous study showed that was overexpressed drug‐resistant epithelial carcinoma its inhibition increased the sensitivity cisplatin. In this study, we demonstrate...
Poor prognosis and chemotherapy tolerance are the main obstacles encountered in treatment of cholangiocarcinoma. Chloroquine (CQ), an antimalarial agent, is able to induce sustained endoplasmic reticulum (ER) stress by functioning as autophagy inhibitor. The present study indicated that CQ had ability apoptosis QBC939 cholangiocarcinoma cells. Furthermore, using western blotting, Hoechst staining flow cytometry, it was demonstrated induced Analysis a polymerase chain reaction (PCR) array...
Bcl-2, which belongs to the Bcl-2 family, is frequently overexpressed in various types of cancer cells and contributes drug resistance. However, function cisplatin resistance human ovarian not fully understood. In this study, we found that pharmacological inhibitor ABT737 or genetic knockdown increased cytotoxicity cisplatin-resistant cells. Additionally, treatment with siRNA cisplatin-induced free Ca2+ levels cytosol mitochondria, endoplasmic reticulum (ER)-associated mitochondria-mediated...
Abstract Increasing evidence suggests that p62/SQSTM1 functions as a signalling centre in cancer. However, the role of p62 tumour development depends on interacting factors it recruits and its precise regulatory mechanism remains unclear. In this study, we investigated pro‐death recruitment with goal improving anti‐tumour drug effects ovarian cancer treatment. We found Caspase 8 high expression is correlated longer survival time compared cases low vivo experiments suggested insoluble...
MiR-216a-5p has opposite effects on tumorigenesis and progression in the context of different tumors, acting as either a tumor suppressor or an oncogene. However, expression function miR-216a-5p pancreatic cancer (PC) is not well characterized. In this study, we found was significantly downregulated PC tissues cell lines, which showed negative correlation with peripancreatic lymph, perineural invasion TNM stage PCs patients. We made use functional assays to reveal that inhibited growth...
The phosphoinositide 3-kinase (PI3K) /AKT/mammalian target of rapamycin (mTOR) signaling pathway is frequently mutated in cancers, leading to increased cell proliferation, migration, and chemoresistance. Currently, a number small molecule inhibitors the PI3K/AKT/mTOR have been assessed preclinical clinical studies. It has found that dual PI3K/mTOR may inhibit proliferation induce apoptosis but mechanism still being explored. Therefore, determining role PKI-402 cancer cells facilitate...