Hypervirulent and multidrug resistant Klebsiella pneumoniae strains pose a significant threat to the public health. In present study, 21 carbapenem-resistant K. isolates (CRKP) were determined by string test as hypermucoviscous (HMKP), with prevalence of 15.0% (21/140) among CRKP, 1.1% (21/1838) all isolates. Among them, 7 (33.3%), 1 (4.76%) isolate belonged capsular serotype K20 K2 respectively, while 13 (61.9%, 13/21) weren't successfully typed serotyping. All carbapenemase-producers...

A distinctive syndrome caused by hypermucoviscous Klebsiella pneumoniae (HMKP) including pyogenic liver abscess (PLA) is now becoming a globally emerging disease. In the present study, 22.8% (84/369) of K. clinical isolates associated with various types invasive infections were identified as HMKP, 45.2% PLA. Multivariate regression analysis showed that male patients 41-50 years, PLA, diabetes mellitus, and hypertension independent risk factors for HMKP infections. K2 (42.9%, 36/84) was most...

Staphylococcus aureus sequence type 9 (ST9) is the main LA-MRSA clone spreading in Asian continent. It can colonize and cause mild to severe infections both animal humans.

Resveratrol is a natural phytoalexin. In recent studies, it has been shown to have beneficial effects on cardiovascular disease and cancer deemed effective antiviral immunomodulatory activities. Methicillin-resistant Staphylococcus aureus multidrug-resistant pathogen associated with skin soft tissue infections. Alpha-hemolysin known play key role in the symptoms caused by S. aureus, saeRS two-component system be major regulatory of virulence. The present study was designed determine effect...

Previous studies have shown that the administration of antibiotics at subinhibitory concentrations stimulates biofilm formation by majority multidrug-resistant Staphylococcus aureus (MRSA) strains. Here, we investigated effect mupirocin on community-associated (CA) mupirocin-sensitive MRSA strain USA300 and highly mupirocin-resistant clinical S. SA01 to SA05 isolates.

Since 2010, methicillin-resistant Staphylococcus aureus (MRSA) ST59 began to increase in prevalence China, gradually replacing ST239 and has become the dominant clone most hospitals China. Here, we investigated changing epidemiology, phylogenetic reconstruction, genomic characterization of MRSA clones China identify driving factors ST59. Most isolates were identified as (36.98%; 277/749), which increased from 25.09% 2014 35.53% 2019. The analysis 749 showed a high level diversity copresence...

Methicillin-resistant Staphylococcus aureus (MRSA) sequence type (ST) 22, especially the epidemic MRSA-15 (EMRSA-15), has been one of most important disease-causing clones transmitting rapidly within and between hospitals globally. However, genetic features evolution Chinese MRSA ST22 remain to be determined. Herein, we performed comparative genomics analysis 12 community-associated (CA) isolates from China with 9 CA-MSSA 284 genomes global sources, clarify genotypic potential transmission...

Stimulator of interferon response cGAMP interactor (STING) is essential for both innate and adaptive immunity. However, a comprehensive molecular characterization STING expression across hematological malignancies lacking. In this study, the pan-blood-cancer landscape related to was identified using GTEx, CCLE, Hemap, TCGA databases, potential value predicting prognosis investigated. The relationship between immune cell enrichment assessed in Hemap database. Moreover, efficacy immunotherapy...

Acute myeloid leukemia (AML) is a heterogeneous disease related to broad spectrum of molecular alterations. The successes immunotherapies treating solid tumors and deeper understanding the immune systems patients with hematologic malignancies have promoted development for treatment AML. And high tumor mutational burden (TMB) an emerging predictive biomarker response immunotherapy. However, association gene mutation in AML TMB immunological features still has not been clearly elucidated. In...

Many countries have reported increasing rates of penicillin-susceptible methicillin-sensitive Staphylococcus aureus (MSSA-PENS). To date, there is relatively little known about the current situation and molecular characteristics MSSA-PENS in China. In this study, we carried out a laboratory-based multi-region retrospective study to investigate genomic epidemiology characterisation isolated from invasive bloodstream infections (BSIs) across 17 provinces. The prevalence isolates increased...

ST59 is the predominant pathotype of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) in China. As a variant ST59, there relatively little known about detailed information ST338. To address this issue, here, we described thirteen ST338 CA-MRSA strains isolated from severe bloodstream infection cases, and focused on their epidemiology, genetic features virulence potential. Phylogenetic analysis showed earliest strain study likely predecessor recent lineage (after...

Staphylococcus epidermidis has emerged as an often encountered pathogen responsible for hospital-acquired infections. The aim of present study is to investigate the microbiological characteristic S. isolates isolated from sterile specimens and skin in a Chinese tertiary hospital. A total 223 non-duplicate were collected various inpatients among 10 years Wenzhou, China. 106 obtained (urethral orifices) healthy volunteers. All tested antimicrobial susceptibility. PCR was used detect virulence-...

Previous studies have mainly focused on outpatient cases of skin and soft tissue infections (SSTIs), with limited attention to inpatient occurrences. Thus, we aimed compare the clinical parameters inpatients SSTIs, performed genomic characterization, determined subtypes Panton-Valentine leucocidin (PVL) bacteriophages methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from these patients. We found that PVL-positive patients had shorter hospital stays (mean, 9 vs. 24 days; p...

Previous studies have shown that livestock (LA)-MRSA ST398 evolved from a human-adapted methicillin-susceptible S. aureus (MSSA) clone. However, detailed information regarding ST9 is still unclear. Here, we characterized community-associated methicillin-resistant Staphylococcus (CA-MRSA) ST9-SCCmec XII isolate has not been previously reported to cause serious disease in China. We obtained whole-genome sequences of one ST9-t899-XII isolate—ZY462471—from patient with bloodstream infection...

Mupirocin, a topical antibiotic, has been utilized for decades to treat Staphylococcus aureus skin infections, as well decolonize patients at risk of methicillin-resistant S. (MRSA) infection. The aims this study were investigate the expression α-toxin (encoded by hla gene) in ten clinical MRSA strains response sub-inhibitory concentration mupirocin (1/32 minimum inhibitory [MIC]) using activity determination and enzyme-linked immune sorbent assay (ELISA). Subsequently, real-time polymerase...

Abstract The role of B7 ‐ H3 in acute myeloid leukemia (AML) is not fully understood. Two previous studies investigating its expression and significances AML are partially different. In this study, we aimed to systematically characterize the genomic immune landscape patients with altered using multi‐omics data public domain. We found significantly increased compared either other hematological malignancies or healthy controls. Clinically, high was associated old age, TP53 mutations, wild‐type...

Staphylococcus epidermidis is a commensal bacterium which widely colonizes in human skin and mucous membrane rarely causes clinically manifested infections. S. surface protein I (SesI) considered to be the major virulence factor of infection, but its pathogenesis not clear. Here, we demonstrated that prevalence sesI among invasive isolates (20.8%, 26/125) was significantly higher than colonizing (3.8%, 4/106). The positive rates biofilm-associated genes (aap, icaA, IS256)...

Previous studies on vancomycin-intermediate Staphylococcus aureus (VISA) have mainly focused drug resistance, the evolution of differences in virulence between VISA and vancomycin-sensitive S. (VSSA) requires further investigation. To address this issue, study, we compared toxin profiles pair groups VSSA strains, including a series vancomycin-resistant induced strains-SA0534, SA0534-V8, SA0534-V16. We established mouse skin infection model to evaluate invasive capacity found that although...

Abstract Background Methicillin resistance in Staphylococcus aureus is primarily due to the mecA gene found highly diverse staphylococcal cassette chromosome mec (SCCmec) elements, with an increasing number of variants being continually discovered. Objectives To characterize two novel SCCmec identified clonal complex (CC) 398 strains and lineage-specific pseudo-SCCmec elements ST88 clone. Methods WGS comparative genomic analysis were used elucidate element diversity representative isolates....

The emergence of nosocomial infections caused by hypervirulent and carbapenem-resistant

The post-antibiotic effect (PAE) of teicoplanin was compared with that vancomycin for five selected Gram-positive cocci. Two concentrations each antibiotic were investigated, the test organisms being exposed to both 1 and 2 h. At a concentration 25 mg/l, had greater PAE than methicillin resistant staphylococci Enterococcus faecalis strains tested. 5 gave equal effects those except strain methicillin-resistant Staphylococcus aureus (MRSA) variation in exposure time only small changes PAE, MRSA