Aims: To evaluate the clinicopathological and immunohistochemical characteristics of Merkel cell carcinoma (MCC) in an attempt to find new, potentially significant, prognostic markers. Methods results: Clinical data follow‐up, histopathological features (pattern, size, thickness, mitoses, vascular invasion, lymphocytic infiltration) detection [CK20, thyroid transcription factor (TTF‐1), chromogranin A, synaptophysin, p53, Ki67, Fli‐1, CD99, c‐Kit] were evaluated 20 cases MCC. Fli‐1 CD99...

The aim of this study is to evaluate possible harmful effects high doses t-pterostilbene (t-PTER) and quercetin (QUER) in Swiss mice. Mice were fed during 28 days at 0, 30, 300, 3000 mg/kg body weight/day t-PTER, QUER, or a mixture both, t-PTER + which are equivalent 5, 50, 500 times, respectively, the estimated mean human intake these polyphenols (25 mg/day). Daily oral administration both them did not cause mortality experimental period. There no differences food water consumption on sex....

Background/Objective Patients with non-small cell lung cancer (NSCLC) develop resistance to antitumor agents by mechanisms that involve the epithelial-to-mesenchymal transition (EMT). This necessitates development of new complementary drugs, e.g., cannabinoid receptors (CB1 and CB2) agonists including tetrahydrocannabinol (THC) cannabidiol (CBD). The combined use THC CBD confers greater benefits, as enhances effects reduces its psychotropic activity. We assessed relationship between...

Implantation of a retrogradely-shed endometrium during menstruation requires an adequate blood supply. The has angiogenic potential, and endometriotic lesions grow in areas with rich vascularization, suggesting that angiogenesis is prerequisite for endometriosis development. Targeting vascular endothelial growth factor (VEGF) leads to inhibition endometriosis. Dopamine its agonists, such as cabergoline (Cb2), promote VEGF receptor-2 (VEGFR-2) endocytosis cells, preventing VEGF–VEGFR-2...

Persistent respiratory syncytial virus (RSV) infections have been associated with the exacerbation of chronic inflammatory diseases, including obstructive pulmonary disease (COPD). This infects epithelium, leading to inflammation, and induces release mucins loss cilia activity, two factors that determine mucus clearance increase in sputum volume. These alterations involve reactive oxygen species-dependent mechanisms. The antioxidant N-acetylcysteine (NAC) has proven useful management COPD,...

Idiopathic pulmonary hypertension (IPAH) is a rare disease characterized by progressive increase in vascular resistance leading to heart failure. MicroRNAs (miRNAs) are small noncoding RNAs that control the expression of genes, including some involved progression IPAH, as studied animals and lung tissue. These molecules circulate freely blood their associated with different pathologies. Here, we profile circulating miRNAs 12 well-characterized IPAH patients using microarrays. We found...

Osteoarthritis is an inflammatory disease in which all joint-related elements, articular cartilage particular, are affected. The poor regeneration capacity of this tissue together with the lack pharmacological treatment has led to development regenerative medicine methodologies including microfracture and autologous chondrocyte implantation (ACI). effectiveness ACI been shown vitro vivo , but use other cell types, bone marrow adipose-derived mesenchymal stem cells, necessary because...

Previous studies in an experimental mouse model of endometriosis have shown that the dopamine agonist (DA) cabergoline (Cb2) reduces angiogenesis and endometriotic lesions, hypothetically binding to receptor type-2 (DRD2). To date, this has not been described human endometrium and/or lesions. Thus, we aimed investigate presence DRD2 said tissues. Endometrium fragments were implanted nude mice treated with different doses Cb2. Polymerase chain reaction assays immunohistochemistry performed...

Abstract Perinatal stem cells such as human umbilical cord Wharton's jelly (HWJSCs) are excellent candidates for tissue engineering because of their proliferation and differentiation capabilities. However, potential into epithelial at in vitro vivo levels has not yet been reported. In this work we have studied the capability HWJSCs to differentiate oral mucosa skin using a bioactive three-dimensional model that mimics native epithelial-mesenchymal interaction. To achieve this, primary cell...

The renal involvement of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been reported. etiology kidney injury appears to be tubular, mainly due the expression angiotensin-converting enzyme 2, key joint receptor for SARS-CoV-2; however, cases with glomerular implication have also documented. multifactorial origin this could include virus-mediated injury, cytokine storm, angiotensin II pathway activation, complement dysregulation, hyper-coagulation, and microangiopathy. We...

Primary ciliary dyskinesia (PCD) is a clinically uniform entity, although cilia motility and structure can vary among patients, making diagnosis difficult. Chronic sinusitis, bronchiectasis, sinus hypoplasia, secretory otitis media, low fertility are common in PCD patients. The aim of this work was to correlate nasal activity with clinical structural abnormalities PCD. A secondary evaluate the usefulness high-resolution digital high-speed video (DHSV) PCD.We analyzed mucociliary transport...

During hyposensitization therapy with aluminium-precipitated antigen solutions, a small % of patients develop persistent subcutaneous nodules at the injection site; existence delayed sensitivity to aluminium has been implicated in pathogenesis these nodules. We studied prevalence (using patch, prick and intradermal tests) common contact allergens (TRUE Test) 20 healthy subjects, 40 treated aluminium-containing extracts, whom had that remained for more than 2 months, other half having no...

This study characterizes the histogenesis of soft tissue Ewing's sarcoma (StEs) based upon an analysis three tumors. Long-term cultured cell lines and nude mice xenografts were established from original neoplasms or their metastases. Histologically they revealed a small round pattern without signs differentiation. Several ultrastructural features neural type found; same also seen on culture lines. Moreover, immunohistochemical for markers presence HNK-1, NSE, LIRC-LON 36, S-100 protein,...

Osteosarcoma (OS) displays complex karyotypes with numerical changes as well structural abnormalities suggesting that several oncogenes and tumor suppressor genes may be implicated in the biology of OS. The aim our study was to investigate possible implication molecular alterations G1 S-phase checkpoint pathogenesis We analyzed samples from 29 patients found RB TP53 6 (21%) 3 (10%) cases, respectively. Homozygous deletion INK4A/ARF locus methylation INK4A detected 2 (7%) CDK4 MDM2...

Matrix-assisted autologous chondrocyte implantation (MACI) has shown promising results for cartilage repair, combining cultured chondrocytes and hydrogels, including alginate. The ability of MACI is limited by different factors donor site morbidity, dedifferentiation, lifespan or poor proliferation in vitro. Mesenchymal stem cells could represent an alternative regeneration. In this study, we propose a scaffold consisting mixed alginate-agarose hydrogel combination with human dental pulp...

Eosinophils are possibly the most important inflammatory cells in pathogenesis of rhinosinusitis with nasal polyposis. Eosinophil degranulation is mechanism by which these exert their action. Knowledge eosinophil state and mode therefore may help us to better understand this disease. A study made using transmission electron microscopy (TEM), attempting establish correlations certain clinical variables considered be importance patients polyposis.A prospective TEM was examine 582 eosinophils...