A5080081719- Plant Molecular Biology Research
- Bioenergy crop production and management
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Genetic Mapping and Diversity in Plants and Animals
- Plant biochemistry and biosynthesis
- Plant Reproductive Biology
- Genetically Modified Organisms Research
- Plant nutrient uptake and metabolism
- Biofuel production and bioconversion
- Insect Resistance and Genetics
- Anaerobic Digestion and Biogas Production
- Animal Genetics and Reproduction
- Genomics and Chromatin Dynamics
- Heat shock proteins research
- Animal Disease Management and Epidemiology
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Bioeconomy and Sustainability Development
- Reproductive Biology and Fertility
- Transgenic Plants and Applications
- RNA Research and Splicing
- Genetic diversity and population structure
Tongren Hospital
2025
Southeast University
2025
Southwest Hospital
2025
Army Medical University
2025
Wuhan University
2021-2024
Research Institute of Forestry
2015-2024
Chinese Academy of Forestry
2015-2024
Nanjing Forestry University
2015-2024
Sichuan Academy of Agricultural Sciences
2023
Guizhou Provincial People's Hospital
2023
Adventitious rooting is an essential step in vegetative propagation. Currently, the mechanism that regulates adventitious root (AR) development woody plants poorly understood. This work demonstrates Populus tomentosa WUSCHEL-related homeobox 5a (PtoWOX5a) transcription factor involved AR poplar. PtoWOX5a was specifically expressed tip and lateral during regeneration from stem segment. Phenotypic complementation experiments indicated can functionally complement AtWOX5 quiescent center (QC)...
Background In plants, 14-3-3 proteins are encoded by a large multigene family and involved in signaling pathways to regulate plant development protection from stress. Although twelve Populus 14-3-3s were identified based on the trichocarpa genome V1.1 previous study, no systematic analysis including organization, gene structure, duplication relationship, evolutionary expression compendium has been conducted latest P. V3.0. Principal Findings Here, comprehensive of is presented. Two new genes...
Heat shock transcription factors (Hsfs), which act as important transcriptional regulatory proteins, play crucial roles in plant developmental processes, and stress responses. Recently, the genome of shrub willow Salix suchowensis was fully sequenced. In this study, a total 27 non-redundant Hsf genes were identified from S. genome. Phylogenetic analysis revealed that members SsuHsf family can be divided into three groups (class A, B, C) based on their structural characteristics. Promoter...
The Na+/H+ antiporters (NHXs) are secondary ion transporters to exchange H+ and transfer the Na+ or K+ across membrane, they play crucial roles during plant development stress responses. To gain insight into functional divergence of NHX genes in poplar, eight PtNHX were identified from Populus trichocarpa genome. PtNHXs containing 10 transmembrane helices (TMH) a hydrophilic C-terminal domain, TMH compose hollow cylinder provide channel for transport. expression patterns cis-acting elements...
Drought is one of the critical factors limiting tree growth and survival. Clarifying adaptation to drought will facilitate cultivation drought-tolerant varieties. Metabolites, as direct signatures biochemical functions, can uncover pathways involved in responses. Here, we investigated physiological metabolic responses Populus simonii drought-susceptible deltoides cv. 'Danhong' drought. Under conditions, P. grew better had a higher photosynthetic rate than 'Danhong'. Global untargeted...
Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) combined with serum carcinoembryonic antigen (CEA) and carbohydrate 19-9 (CA19-9) levels to evaluate the efficacy of colorectal cancer liver metastasis (CRCLM) treatment is still rare.
Abstract Extensive epigenetic reprogramming involves in memory CD8+ T-cell differentiation. The elaborate rewiring underlying the heterogeneous functional states of T cells remains hidden. Here, we profile single-cell chromatin accessibility and map enhancer-promoter interactomes to characterize differentiation trajectory cells. We reveal that under distinct regulations, early activated divergently originated for short-lived effector precursor also uncover a defined leading conversion from...
Heat shock transcription factor (Hsf) family is one of the most important regulators in plant kingdom. Hsf has been demonstrated to be involved various processes associated with growth, development as well response hormone and abiotic stresses. In this study, we carried out a comprehensive analysis desert poplar, Populus euphratica. Total 32 genes encoding were identified they classified into three main classes (A, B, C). Gene structure conserved motif analyses indicated that members each...
Salix psammophila is a desert shrub willow that has extraordinary adaptation to abiotic stresses and plays an important role in maintaining local ecosystems. Moreover, S. regarded as promising biomass feedstock because of its high yields short rotation coppice cycle. However, few suitable reference genes (RGs) for quantitative real-time polymerase chain reaction (qRT-PCR) constrain the study on normalization gene expression until now. Here, we investigated stabilities 14 candidate RGs across...
Understanding the genetic architecture of adventitious root and related shoot traits will facilitate cultivation superior genotypes. In this study, we measured 12 434 F1 genotypes originating from Populus deltoides ‘Danhong’ × simonii ‘Tongliao1’ conducted an integrative analysis quantitative trait locus (QTL) mapping RNA-Seq data to dissect their regulatory genes. Extensive segregation, high repeatability, significant correlation relationship were detected for investigated traits. A total...
Abstract Populus euphratica is a dominant tree species in desert riparian forests and possesses extraordinary adaptation to salinity stress. Exploration of its genomic variation molecular underpinning tolerance important for elucidating population evolution identifying stress-related genes. Here, we identify approximately 3.15 million single nucleotide polymorphisms using whole-genome resequencing. The natural populations P. northwest China are divided into four distinct clades that exhibit...
Abstract Adventitious root (AR) development is an extremely complex biological process that affected by many intrinsic factors and extrinsic stimuli. Some WUSCHEL-related homeobox (WOX) transcription have been reported to play important roles in AR development, but their functional relationships with auxin signaling are poorly understood, especially the developmental plasticity of roots response adversity stress. Here, we identified WOX11/12a–SMALL AUXIN UP RNA36 (SAUR36) module mediates...
Abstract Background Understanding gene regulatory networks has become one of the central research problems in bioinformatics. More than thirty algorithms have been proposed to identify DNA sites during past years. However, prediction accuracy these is still quite low. Ensemble emerged as an effective strategy bioinformatics for improving by exploiting synergetic capability multiple algorithms. Results We a novel clustering-based ensemble algorithm named EMD de novo motif discovery combining...
Maternal effect genes and encoding proteins are necessary for nuclear reprogramming zygotic genome activation. However, the mechanisms that mediate these functions largely unknown. Here we identified XM_359149, a Zar1-like gene is predominantly expressed in oocytes zygotes, which designated (Zar1l). ZAR1L-EGFP formed multiple cytoplasmic foci late two-cell-stage embryos. Expression of ZAR1L C-terminus induced embryonic arrest, accompanied with abnormal methylation histone H3K4me2/3...
The continuous and non-synchronous nature of postnatal male germ-cell development has impeded stage-specific resolution molecular events mammalian meiotic prophase in the testis. Here juvenile onset spermatogenesis mice is analyzed by combining cytological transcriptomic data a novel computational analysis that allows decomposition transcriptional programs spermatogonia substages. Germ cells from testes individual were obtained at two-day intervals 8 to 18 days post-partum (dpp), prepared as...
Abstract Salix psammophila , a sandy shrub known as desert willow, is regarded potential biomass feedstock and plays an important role in maintaining local ecosystems. However, lack of genomic data efficient molecular markers limit the study its population evolution genetic breeding. In this study, chromosome counts, flow cytometry SSR analyses indicated that S. tetraploid. A total 6,346 EST-SSRs were detected based on 71,458 de novo assembled unigenes from transcriptome data. Twenty-seven...
Rab proteins form the largest family of Ras superfamily small GTP-binding and regulate intracellular trafficking pathways. However, function in woody species is still an open question. Here, a total 67 PtRabs were identified Populus trichocarpa categorized into eight subfamilies (RabA-RabH). Based on their chromosomal distribution duplication blocks genome, 27 PtRab paralogous pairs all them generated by whole-genome events. Combined expression correlation date, that keeping highly similar...
The plant leaf, the main organ of photosynthesis, is an important regulator growth. To explore difference between leaf size Populusdeltoides ‘Danhong’ (Pd) and Populus simonii ‘Tongliao1’ (Ps), we investigated length, width, thickness, area, mass per area (LMA), cell leaves from two genotypes profiled transcriptome-wide gene expression patterns through RNA sequencing. Our results show that Pd was significantly larger than Ps, but epidermal smaller Ps. caused by numbers. Transcriptome...
Polyploid plants typically display advantages on some agronomically important traits over their diploid counterparts. Extensive studies have shown genetic, transcriptomic, and epigenetic dynamics upon polyploidization in multiple plant species. However, few unveiled those alternations imposed only by ploidy level, without any interference from heterozygosity. Cultivated potato is highly heterozygous. Thus, this study, we developed two homozygous autotetraploid lines one line parallel a...
Summary Wood is resulted from the radial growth paced by division and differentiation of vascular cambium cells in woody plants, phytohormones play important roles activity. Here, we identified that PagJAZ5, a key negative regulator jasmonate (JA) signaling, plays enhancing cell mediating cytokinin signaling poplar 84K ( Populus alba × glandulosa ). PagJAZ5 preferentially expressed developing phloem cambium, weakly xylem cells. Overexpression (OE) PagJAZ5m (insensitive to JA) increased...