A5061488176- Cancer Research and Treatments
- Hepatitis B Virus Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Liver Disease Diagnosis and Treatment
- Amino Acid Enzymes and Metabolism
- Cancer, Hypoxia, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Hepatitis C virus research
- Cancer Cells and Metastasis
- Microtubule and mitosis dynamics
- Immunotherapy and Immune Responses
- Melanoma and MAPK Pathways
- Liver physiology and pathology
- Mechanisms of cancer metastasis
- Endoplasmic Reticulum Stress and Disease
- RNA Interference and Gene Delivery
- Pancreatic and Hepatic Oncology Research
- Cancer Mechanisms and Therapy
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- PI3K/AKT/mTOR signaling in cancer
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Cancer, Lipids, and Metabolism
- Virus-based gene therapy research
China Medical University
2017-2025
China Medical University Hospital
2017-2025
National Health Research Institutes
2013-2023
National Research Institute of Brewing
2017
National Institute of Infectious Diseases
2013
National Cheng Kung University
2005-2013
Ground glass hepatocytes (GGH) in chronic hepatitis B virus (HBV) infection harbor HBV pre-S deletion mutants endoplasmic reticulum (ER) and exhibit complex biologic features such as ER stress, DNA damage, growth advantage. The presence of serum has been shown to predict the development hepatocellular carcinoma (HCC) carriers. GGHs hence represent a potentially preneoplastic lesion. Whether specific factor is overexpressed activated remains be clarified. In this study, factor(s) up-regulated...
Hepatocellular carcinoma (HCC) is among the most common and lethal human cancers worldwide. Despite remarkable advances in treatment, high mortality HCC patients remains a big challenge. To develop novel therapeutic strategies for thus urgently needed to improve patient survival. Dendritic cells (DC)-based vaccines can induce tumor-specific immunity have emerged as promising approach treating patients; however, its effectiveness needs be improved. Recently, blockade of programmed death...
Ground glass hepatocytes (GGHs) harboring hepatitis B virus (HBV) pre-S mutants have been recognized as precursor lesions of hepatocellular carcinoma (HCC). Previously, we observed the activation mammalian target rapamycin (mTOR) in GGHs and HCCs, together with a decreased expression HBV surface antigen (HBsAg) HCC tissues. It is, therefore, hypothesized that mTOR during tumorigenesis may potentially down-regulate HBsAg expression. In this study, verified an inverse relationship between...
Hepatitis B virus (HBV) pre-S2 mutant can induce hepatocellular carcinoma (HCC) via the induction of endoplasmic reticulum stress to activate mammalian target rapamycin (MTOR) signaling. The association metabolic syndrome with HBV-related HCC raises possibility that mutant-induced MTOR activation may drive development disorders promote tumorigenesis in chronic HBV infection. To address this issue, glucose metabolism and gene expression profiles were analyzed transgenic mice livers harboring...
Abstract Chronic hepatitis B virus (HBV) infection is a major risk factor for the development of hepatocellular carcinoma (HCC), leading cause cancer mortality worldwide. Hepatitis X protein (HBx) and pre-S2 mutant have been proposed as two most important HBV oncoproteins that play key roles in HCC pathogenesis. Curcumin botanical constituent displaying potent anti-inflammatory anti-cancer properties without toxic side effects. Phytosomal formulation curcumin has shown to exhibit enhanced...
Overexpression of the serine/threonine kinase GLK/MAP4K3 in human lung cancer is associated with poor prognosis and recurrence, however, role GLK recurrence remains unclear. Here, we report that transgenic promotes tumor metastasis cell migration through scaffold protein IQ motif-containing GTPase-activating 1(IQGAP1). mice displayed enhanced distant metastasis. IQGAP1 was identified as a GLK-interacting protein; two proline-rich regions WW domain mediated this interaction. colocalized at...
Hepatitis B virus (HBV) DNA integration into the host cell genome is reportedly a major cause of liver cancer, and source hepatitis surface antigen (HBsAg). High HBsAg levels can alter immune responses which therefore contributes to progression HBV-related disease. However, what extent leads persistent circulating unclear. Here, we aimed determine if HBV associated with persistence in people exposed HBV. We established digital droplet quantitative inverse PCR (dd-qinvPCR) method quantify...
ABSTRACT The development of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) has been found to be associated with disturbed lipid metabolism. To elucidate the role metabolism in HBV tumorigenesis, we investigated dynamic pattern pre-S2 mutant-induced tumorigenesis. Lipid and gene expression profiles were analyzed an vitro culture system transgenic mouse livers harboring mutant. mutant showed a biphasic accumulation, starting from mild fatty change early (1 month) livers, which...
Abstract Citrate synthase (CS), the first and rate‐limiting enzyme of tricarboxylic acid (TCA) cycle, plays a decisive role in regulating energy generation mitochondrial respiration. Most proteins are synthesized cytoplasm as preproteins with an amino (N)‐terminal targeting sequence (MTS) that directs mitochondria‐specific sorting preprotein. However, MTS mechanism human CS protein not fully characterized. The gene is single nuclear which transcribes into two mRNA variants, isoform (CSa) b...
Hepatocellular carcinoma (HCC) is among the most common and lethal human cancers worldwide. Despite curative resection, high recurrence of HCC remains a big threat, leading to poor patient outcomes. Hepatitis B virus (HBV) pre-S mutants, which harbor deletions over pre-S1 pre-S2 gene segments large surface proteins, have been implicated in recurrence. Therefore, reliable approach for detection mutants urgently needed predicting improve survival. In this study, we used next-generation...
Hepatocellular carcinoma (HCC) remains a serious cause of cancer-related deaths worldwide. Developing new therapeutic strategies is urgently needed to improve the outcomes HCC patients. Dendritic cell (DC)-based vaccines and programmed death 1 (PD-1) immune checkpoint inhibitors have been regarded as potential immunotherapeutics for HCC. However, efficacy combining these two treatments be evaluated.In this study, DCs were derived from mouse bone marrow pulsed with lysates generate DC...
Metabolic syndrome has closely linked to the development of human hepatocellular carcinoma (HCC). By using hepatitis B virus (HBV) X (HBx) transgenic mouse model, we studied dynamic evolution serum and liver profiles lipids global cDNA expression at different stages HBx tumorigenesis. We observed that lipid (triglycerides, cholesterol, fatty acids) revealed a biphasic response pattern during progression tumorigenesis: small peak early phase large or terminal switch tumor phase. analyzing...
Arginine biosynthesis and nitric oxide (NO) production are important for cancer homeostasis. Degradation of arginine may be used to inhibit liver tumors with low argininosuccinate synthetase (ASS) expression. In this report, we investigated an alternative therapeutic approach by targeting lyase (ASL). ASL is transcriptionally induced endoplasmic reticulum stress overexpressed in some human tumors. Knockdown expression short hairpin RNA (shRNA) three cell lines, ML-1, HuH-7, HepG2, decreased...