A5059637012- Plant Molecular Biology Research
- Soybean genetics and cultivation
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Legume Nitrogen Fixing Symbiosis
- Plant Gene Expression Analysis
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Genetic and Mutation Studies
- Technology and Human Factors in Education and Health
- Quantum Dots Synthesis And Properties
- Mass Spectrometry Techniques and Applications
- Nanoparticles: synthesis and applications
- Sunflower and Safflower Cultivation
- Toxic Organic Pollutants Impact
- Rice Cultivation and Yield Improvement
- Social Media and Politics
- Diverse Interdisciplinary Research Innovations
- Education, Psychology, and Complexity Research
- Lipid metabolism and biosynthesis
- Probiotics and Fermented Foods
Jilin Agricultural University
2014-2024
The TGA transcription factors, a subfamily of bZIP group D, play crucial roles in various biological processes, including the regulation growth and development as well responses to pathogens abiotic stress. In this study, 27 genes were identified soybean genome. expression patterns GmTGA showed that several are differentially expressed under drought salt stress conditions. Among them, GmTGA17 was strongly induced by both stress, which verificated promoter-GUS fusion assay. encodes...
Stress associated proteins (SAPs) containing A20/AN1 zinc finger domains have emerged as novel regulators of stress responses. In this study, 27 SAP genes were identified in soybean. The phylogenetic relationships, exon-intron structure, domain chromosomal localization, putative cis-acting elements, and expression patterns SAPs various tissues under abiotic stresses analyzed. Among the soybean genes, GmSAP16 was significantly induced by water deficit stress, salt, abscisic acid (ABA)...
Flowering time is a trait vital to the adaptation of flowering plants different environments. Here, we report that CCT domain genes play an important role in maize (Zea mays L.). Among 53 family identified maize, 28 were located quantitative locus regions and 15 significantly associated with time, based on candidate-gene association mapping analysis. Furthermore, gene named ZmCOL3 was shown be repressor flowering. Overexpressing delayed by approximately 4 d, either long-day or short-day...
Soybean (Glycine max) is one of the important oil crops worldwide. In recent years, environmental stresses such as drought and soil salinization have severely deteriorated soybean yield quality. We investigated overexpression transcription factor GmTGA15 in response to stress transgenic hairy roots Arabidopsis plants. The results quantitative real time polymerase chain reaction (qRT-PCR) analyses showed that was greatly induced by salt, PEG6000, salicylic acid (SA), gibberellic (GA),...
Domain of unknown function 4228 (DUF4228) proteins are a class widely found in plants, playing an important role response to abiotic stresses. However, studies on the DUF4228 family soybean ( Glycine max L.) sparse. In this study, we identified total 81 genes genome, named systematically based their chromosome distributions. Results showed that these were unevenly distributed 20 chromosomes soybean. The predicted three groups (Groups I–III) maximum likelihood phylogenetic tree. Genetic...
NAC transcription factors are commonly involved in the plant response to drought stress. A transcriptome analysis of root samples soybean variety 'Jiyu47' under stress revealed evidently up-regulated expression GmNAC19, consistent with pattern by quantitative real-time PCR analysis. The overexpression GmNAC19 enhanced tolerance Saccharomyces cerevisiae INVSc1. seed germination percentage and growth transgenic Arabidopsis thaliana were improved comparison those wild type, while composite line...
Drought-induced (Di19) proteins played important roles in plant growth, development, and abiotic stress responses. In the present study, a total of seven Di19 genes were identified soybean. Each soybean gene showed specific responses to salt, drought, oxidative, ABA stresses based on expression profiles. With relatively higher transcript level among members under four treatments, GmDi19-5 was selected for detailed analysis. Inhibitor assays revealed that inhibitor (Fluridone) or H2O2 (DMTU)...
Safflower (Carthamus tinctorius L.), an important traditional Chinese medicine, is cultured widely for its pharmacological effects, but little known regarding the genes related to metabolic regulation of safflower’s yellow pigment. To investigate safflor biosynthesis, 454 pyrosequencing flower RNA at different developmental stages was performed, generating large databases.In this study, we analyzed sequencing data from flowering in safflower. In total, 1,151,324 raw reads and 1,140,594 clean...
Roots are generally the critical drought sensors, but little is known about their molecular response to stress. We used drought-tolerant soybean variety 'Jiyu 47' investigate differentially expressed proteins (DEPs) in roots during seedling stage based on tandem mass tag (TMT) proteomics analysis. Various expression patterns were observed a total of six physiological parameters. A 468 DEPs (144 up-regulated and 324 down-regulated) among 8687 identified stress 24 h. The was further validated...
2,4,6-Trinitrotoluene (TNT) is an aromatic pollutant that difficult to be degraded in the natural environment. The screening of efficient degrading bacteria for bioremediation TNT has received much attention from scholars. In this paper, transcriptome analysis bacterium Buttiauxella sp. S19-1 revealed monooxygenase gene (BuMO) was significantly up-regulated during degradation. S-ΔMO (absence BuMO mutant) 1.66-fold less efficiently than strain (from 71.2% 42.9%), and E-MO mutant (Escherichia...
Two saline-alkali-tolerant bacterial strains, Priestia aryabhattai JL-5 and Staphylococcus pseudoxylosus XW-4, were isolated, with high capabilities of hydrolyzing phosphate producing cellulase, respectively. The molecular mechanisms regulating the saline-alkali tolerance in strain further investigated using transcriptome analysis. contents lactic acid proline enzymatic activity glutamine synthetase significantly increased. properties soils improved by enhanced growth indicator plant Leymus...
Previously, it was reported that miR396s interact with growth-regulating factors (GRFs) to modulate plant growth, development, and stress resistance. In soybean, 11 gma-miR396 precursors (Pre-miR396a-k) were found, 24 GmGRFs predicted as targets of seven mature gma-miR396s (gma-miR396a/b/c/e/h/i/k). To explore the roles miR396-GRF module in low water availability response we analyzed expression Pre-miR396a-k, found Pre-miR396a/i/bdgk/e/h up-regulated leaves down-regulated roots; on contrary,...
Plant roots play crucial roles in their response to drought conditions. However, the molecular responses soybean stress remain unclear. We investigated alterations protein expression of a drought-resistant cultivar ‘Jiyu 47’ during seedling phase based on tandem mass tag (TMT) proteomics analysis. The results revealed significant variations proteins involved several metabolic pathways roots, including sucrose metabolism, abscisic acid (ABA) and ATP-binding cassette (ABC) transporter pathway....
Leymus chinensis (Trin.) Tzvel. is a perennial rhizome grass of the Poaceae (also called Gramineae) family, which adapts well to drought, saline and alkaline conditions. However, little known about stress tolerance L. at molecular level. microRNAs (miRNAs) are play critical roles in nutrient homeostasis, developmental processes, pathogen responses, abiotic plants. In this study, we used Solexa sequencing technology generate high-quality small RNA data from three groups: control group,...
The amino acid permease (AAP) is an important transmembrane protein that involved in the absorption and transport of acids plants. We investigated expression patterns AtAAP genes Arabidopsis thaliana, based on quantitative real-time PCR. results revealed differential eight different tissues, with five (AtAAP1, AtAAP2, AtAAP6, AtAAP7, AtAAP8) expressed at relatively high levels both flowers siliques, suggesting their shared functions accumulation acids. In transgenic plants,...
With the rapid advances in next-generation sequencing technology, numerous non-protein-coding transcripts have been identified, including long noncoding RNAs (lncRNAs), which are functional comprising more than 200 nucleotides. Although lncRNA-mediated regulatory processes extensively investigated animals, there has considerably less research on plant lncRNAs. Nevertheless, multiple studies major crops showed lncRNAs involved crucial processes, growth and development, reproduction, stress...
Soybean transcription factor GmNAC plays important roles in plant resistance to environmental stresses. In this study, GmNAC3 was cloned the drought tolerant soybean variety "Jiyu47", with molecular properties of characterized establish its candidacy as a NAC factor. The yeast self-activation experiments revealed transcriptional activation activity GmNAC3, which localized nucleus by subcellular localization analysis. highest expression detected roots podding stage soybean, and seedlings...
The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, named rbcS promoter. promoter fused with GUS introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, highest expression levels were observed in leaves, activity 7.13- 7.40-fold higher leaves than stems roots, respectively. Moreover, stimulated by light....
This paper discusses the background, necessity and thoughts of constructing virtual simulation teaching experiment center in view existing problems practice bio-agriculture combined with training objectives "Top-Notch Innovative Agriculture Forestry Talents Training Model Reform Project".The integration agricultural science is great significance to improve students' professional accomplishment innovation ability promote reform. Background Proposing Construction Professional Virtual...
Phospholipid N-methyltransferase (PLMT) plays an important role in the synthesis of phosphatidylcholine (PtdCho). The aim this study was to characterize molecular properties GmPLMT and expression soybean its effects on production lipid metabolites. Results showed that composed mainly α-helix a hydrophobic transmembrane protein. In leaves, highly expressed during seedling flowering stages. transgenic Arabidopsis thaliana, highest lowest levels were detected at maturity stages, respectively....