A5016810968- Asthma and respiratory diseases
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Allergic Rhinitis and Sensitization
- RNA modifications and cancer
- Veterinary Equine Medical Research
- Cytokine Signaling Pathways and Interactions
- Exercise and Physiological Responses
- Eosinophilic Esophagitis
- Aluminum toxicity and tolerance in plants and animals
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- Inflammatory mediators and NSAID effects
- interferon and immune responses
- Growth Hormone and Insulin-like Growth Factors
- Cancer-related molecular mechanisms research
- Macrophage Migration Inhibitory Factor
- NF-κB Signaling Pathways
- RNA and protein synthesis mechanisms
- Pediatric health and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
University of Liège
2015-2024
Walloon Excellence in Lifesciences and Biotechnology
2021
Cellular Research (United States)
2014-2020
The Netherlands Cancer Institute
2006-2017
University of Veterinary Medicine
2016
Molecular Oncology (United States)
2013
Physiol (Belgium)
2004-2007
Institut Pasteur de Lille
2005
Université Libre de Bruxelles
2005
UCLouvain
2003
Increases in eosinophil numbers are associated with infection and allergic diseases, including asthma, but there is also evidence that eosinophils contribute to homeostatic immune processes. In mice, the normal lung contains resident (rEos), their function has not been characterized. Here, we have reported steady-state pulmonary rEos IL-5–independent parenchymal Siglec-FintCD62L+CD101lo cells a ring-shaped nucleus. During house dust mite–induced airway allergy, features remained unchanged,...
The respiratory tract is continuously exposed to both innocuous airborne antigens and immunostimulatory molecules of microbial origin, such as LPS. At low concentrations, LPS can induce a lung DC-driven Th2 cell response harmless inhaled antigens, thereby promoting allergic asthma. However, only small fraction people environmental develop What prevents most from mounting upon exposure not understood. Here we have shown that interstitial macrophages (IMs), population with no previously...
Interferons (IFNs) are a group of cytokines with well-established antiviral function. They can be induced by viral infection, secreted and bind to specific receptors on the same or neighbouring cells activate expression hundreds IFN stimulated genes (ISGs) Type I has been known for more than half century. However, recently, type III (IFNλ, IL-28/29) was shown play similar role particularly important at epithelial surfaces. Here we show that airway epithelia, primary target influenza A virus,...
Metastasis confronts clinicians with two major challenges: estimating the patient's risk of metastasis and identifying therapeutic targets. Because they are key signal integrators connecting cellular processes to clinical outcome, we aimed identify transcriptional nodes regulating cancer cell metastasis. Using rodent xenograft models that previously developed, identified transcription factor Fos-related antigen-1 (Fra-1) as a coordinator Fra-1 often is overexpressed in human metastatic...
Quantitative and qualitative changes in mRNA translation occur tumor cells support cancer progression metastasis. Posttranscriptional modifications of transfer RNAs (tRNAs) at the wobble uridine 34 (U34) base are highly conserved contribute to fidelity. Here, we show that ELP3 CTU1/2, partner enzymes U34 mcm5s2-tRNA modification, up-regulated human breast cancers sustain Elp3 genetic ablation strongly impaired invasion metastasis formation PyMT model invasive cancer. Mechanistically, CTU1/2...
Abstract Knockout mice studies have revealed that NF-κB plays a critical role in Th2 cell differentiation and is therefore required for induction of allergic airway inflammation. However, the questions whether also effector phase allergy inhibiting could therapeutic value treatment established asthma remain unanswered. To address these issues, we assessed OVA-sensitized wild-type effects selectively antagonizing activity lungs during OVA challenge. Intratracheal administration decoy...
Abstract Dendritic cells (DCs) are professional APCs that have a unique capacity to initiate primary immune responses, including tolerogenic responses. We genetically engineered bone marrow-derived DCs express the immunosuppressive cytokine IL-10 and tested ability of these control experimental asthma. A single intratracheal injection OVA-pulsed IL-10-transduced (OVA-IL-10-DCs) naive mice before OVA sensitization challenge prevented all cardinal features airway allergy, namely, eosinophilic...
Abstract Sirtuins are a unique class of NAD+-dependent deacetylases that regulate diverse biological functions such as aging, metabolism, and stress resistance. Recently, it has been shown sirtuins may have anti-inflammatory activities by inhibiting proinflammatory transcription factors NF-κB. In contrast, we report in this study pharmacological inhibition dampens adaptive Th2 responses subsequent allergic inflammation interfering with lung dendritic cell (DC) function mouse model airway...
Lyme disease is caused by spirochetes of the Borrelia burgdorferi sensu lato complex. They are transmitted mainly Ixodes ricinus ticks. After a few hours infestation, neutrophils massively infiltrate bite site. can kill via phagocytosis, oxidative burst, and hydrolytic enzymes. However, factors in tick saliva promote propagation bacteria host even presence large number neutrophils. The neutrophil extracellular trap (NET) consists extrusion neutrophil's own DNA, forming traps that retain...
Conventional dendritic cells (DCs) are considered to be the prime initiators of airway allergy. Yet, it remains unclear whether specific DC subsets preferentially involved in allergic sensitization. Here, we systematically assessed respective pro-allergic potential individually sorted lung isolated from house dust mite antigen (HDM)-treated donor mice, following transfer naïve recipients. Transfer CD11c+CD11b+ DCs, but not CD11c+CD11b−CD103+ was sufficient The subpopulation composed...
It has been shown that γδ T cells protect against the formation of squamous cell carcinoma (SCC) in several models. However, role human papillomavirus (HPV)-associated uterine cervical SCC, third-leading cause death by cancer women, is unknown. Here, we investigated impact a transgenic mouse model carcinogenesis induced HPV16 oncoproteins. Surprisingly, promoted development oncoprotein-induced lesions. oncoproteins decrease epidermal Skint1 expression and associated antitumor Vγ5
Abstract Neutrophils rapidly undergo spontaneous apoptosis following their release from the bone marrow. Although central to leukocyte homeostasis, mechanisms that regulate neutrophil remain poorly understood. We show here of cultured neutrophils is preceded by a substantial increase in intracellular levels 16 and 24 carbon atom (C16- C24)-ceramides, which are lipid second messengers stress signaling. Treatment with fumonisin B2, selective inhibitor de novo pathway ceramide synthesis,...
Regulation of mRNA translation elongation impacts nascent protein synthesis and integrity plays a critical role in disease establishment. Here, we investigate features linking regulation codon-dependent to expression homeostasis. Using knockdown models enzymes that catalyze the mcm5s2 wobble uridine tRNA modification (U34-enzymes), show gene codon content is necessary but not sufficient predict fate. While defects upon perturbation U34-enzymes are strictly dependent on content, consequences...
Abstract Macrophage polarization is a process whereby macrophages acquire distinct effector states (M1 or M2) to carry out multiple and sometimes opposite functions. We show here that translational reprogramming occurs during macrophage this relies on the Elongator complex subunit Elp3, an enzyme modifies wobble uridine base U34 in cytosolic tRNAs. Elp3 expression downregulated by classical M1‐activating signals myeloid cells, where it limits production of pro‐inflammatory cytokines via...