A5007968030- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Evolution and Paleontology Studies
- Genetic diversity and population structure
- Mitochondrial Function and Pathology
- Bat Biology and Ecology Studies
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- Protist diversity and phylogeny
- Gut microbiota and health
- RNA modifications and cancer
- Primate Behavior and Ecology
- Adipose Tissue and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Zebrafish Biomedical Research Applications
- Lipid metabolism and biosynthesis
- Plant Disease Resistance and Genetics
- Muscle Physiology and Disorders
- Pleistocene-Era Hominins and Archaeology
- Biofuel production and bioconversion
- Isotope Analysis in Ecology
- Anaerobic Digestion and Biogas Production
- Dysphagia Assessment and Management
- Cancer, Hypoxia, and Metabolism
- Plant Pathogenic Bacteria Studies
Deakin University
2016-2024
Charles River Laboratories (Germany)
2024
La Trobe University
2003
Skeletal muscle mitochondria adaptation to exercise training is mediated by molecular factors that are not fully understood. Mitochondria import over 1000 proteins encoded the nuclear genome, but RNA population resident within organelle generally thought be exclusively mitochondrial genome. However, recent in vitro evidence suggests specific nuclear-encoded miRNAs and other non-coding RNAs (ncRNAs) can reside matrix. Whether these present of skeletal tissue, if this affected endurance - a...
Complete genomes of xenobiotic-degrading microorganisms provide valuable resources for researchers to understand molecular mechanisms involved in bioremediation. Despite the well-known ability Sphingomonas paucimobilis degrade persistent xenobiotic compounds, a complete genome sequencing is lacking this organism. In line with this, we report first sequence (strain AIMST S2), an organophosphate and hydrocarbon-degrading bacterium isolated from oil-polluted soil at Kedah, Malaysia. The was...
The IL-2 family of cytokines act via receptor complexes that share the interleukin-2 gamma common (IL-2Rγc) chain to play key roles in lymphopoiesis. Inactivating IL-2Rγc mutations results severe combined immunodeficiency (SCID) humans and other species. This study sought generate an equivalent zebrafish SCID model. il2rga gene was targeted for genome editing using TALENs presumed loss-of-function alleles analyzed with respect immune cell development impacts on intestinal microbiota tumor...
The evolution of Australia's distinctive marsupial fauna has long been linked to the onset continent-wide aridity. However, how this profound climate change event affected diversification extant lineages is still hotly debated. Here, we assemble a DNA sequence dataset Macropodoidea-the clade comprising kangaroos and their relatives-that incorporates complete mitogenome for Desert 'rat-kangaroo', Caloprymnus campestris. This enigmatic species went extinct nearly 90 years ago known from...
Mitochondria are central to cellular function, particularly in metabolically active tissues such as skeletal muscle. Nuclear-encoded RNAs typically localize within the nucleus and cytosol but a small population may also translocate subcellular compartments mitochondria. We aimed investigate nuclear-encoded that mitochondria of muscle cells tissue. Intact were isolated via immunoprecipitation (IP) followed by enzymatic treatments (RNase-A proteinase-K) optimized remove transcripts located...
A simple method for tracing carbon fixation and lipid synthesis in microalgae was developed using a combination of solid-phase extraction (SPE) negative ion chemical ionisation gas chromatography mass spectrometry (NCI-GC-MS). NCI-GC-MS is an extremely sensitive technique that can produce unfragmented molecular making this particularly useful stable isotope enrichment studies. Derivatisation fatty acids pentafluorobenzyl bromide (PFBBr) allows the coupling high separation efficiency GC...
A Gram-negative, filamentous aerobic bacterium designated as strain Mgbs1T was isolated on 12 April 2017 from the subsurface soil and leaf litter substrate at base of a Koompassia malaccensis tree in tropical peat swamp forest northern regions state Selangor, Malaysia (3° 39' 04.7' N 101° 17' 43.7'' E). Phylogenetic analyses based full 16S rRNA sequence revealed that belongs to genus Chitinophaga with greatest similarity terrae KP01T (97.65 %), jiangningensis DSM27406T (97.58 dinghuensis...
Understanding the molecular basis of plant heat tolerance helps to predict consequences climate warming on performance, particularly in vulnerable environments. Our current understanding comes primarily from studies Arabidopsis thaliana (Arabidopsis) and select crops exposed short intense periods stress. In this study we sought characterise transcriptomic responses Wahlenbergia ceracea (W. ceracea), an Australian alpine herb, growth under sustained moderate warming. We compared pre-defined...
Marine heterotrophic protists of the Labyrinthulomycota are interest for their biotechnological (e.g. thraustochytrid production lipids) and ecological wasting disease rapid blight by pathogens genus Labyrinthula) applications; culture-based laboratory studies a central technique this research. However, maintaining such microorganism cultures can be labour- cost-intensive, with high risk culture contamination die-off over time. Deep-freeze storage, or cryopreservation, used to maintain...
The mitochondrial genome of the rare endemic New Guinean dasyurid Phascolosorex doriae (Thomas 1886) has been used to clarify relationships within ‘phascolosoricinae’. typical gene arrangement seen in other marsupials. Molecular analyses using complete mitogenomes dasyurids resolve red-bellied dasyure as sister narrow-striped dorsalis and show that these two species diverged early Pliocene. invasion emergent rainforest habitats (in late Miocene) by common ancestor Ph. doriae, Neophascogale...
ABSTRACT Initially thought to localise at the cytosol and nucleus only, emerging evidence indicates that miRNAs also within mitochondria where they could regulate diverse pathological physiological processes. Therefore, aim of current study was use small RNA sequencing profile compare entire population in human skeletal muscle healthy males whole-tissue isolated rest response acute endurance exercise. Twelve (age 26 ± 4 years, mean SD) cycled for 60 min 70% VO 2peak biopsies were collected...
We characterized the complete genome sequence of lytic Salmonella enterica bacteriophage PRF-SP1, isolated from Penang National Park, a conserved rainforest in northern Malaysia. The novel phage species Autographiviridae family has 39,966-bp double-stranded DNA (dsDNA) containing 49 protein-encoding genes and shares 90.96% similarity with Escherichia DY1.
Abstract Background The role of oral and gut microbiomes in the pathogenesis schizophrenia has recently come to light with advancement sequencing technology. Recent studies have shown that patients different microbiome profiles than healthy people. interaction microbiota brain is facilitated through gut-brain axis. Several profiled Malaysian population, however none investigated its relationship etiology schizophrenia. aim this preliminary study therefore investigate between population based...
The analysis of 13C-labeled lipids by mass spectrometry is challenging due to the complexity from labeling large number carbon atoms in lipids. To further add complexity, different adducts can be produced during electrospray ionization and in-source fragmentation, which create complex overlapping isotope patterns that only resolved using high-resolution spectrometry. Co-elution even after chromatographic separation also adds potential for spectra. Here, we describe a procedure enables full...
ABSTRACT Mitochondria are central to cellular function, particularly in metabolically active tissues such as skeletal muscle. Nuclear-encoded RNAs typically localise within the nucleus and cytosol but a small population may also translocate subcellular compartments mitochondria. We aimed investigate nuclear-encoded that mitochondria of muscle cells tissue. Intact were isolated via immunoprecipitation (IP) followed by enzymatic treatments (RNase-A proteinase-K) optimised remove transcripts...
Salmonella infections across the globe are becoming more challenging to control due emergence of multidrug-resistant (MDR) strains. Lytic phages may be suitable alternatives for treating these infections. Most date were collected from human-impacted environments. To further explore phage space, and potentially identify with novel characteristics, we characterized Salmonella-specific isolated Penang National Park, a conserved rainforest. Four broad lytic spectrum (kills >5 serovars)...
We present the genome of living fossil,
Abstract Labyrinthula spp. are saprobic, marine protists that also act as opportunistic pathogens and the causative agents of seagrass wasting disease (SWD). Despite threat local- large-scale SWD outbreaks, there currently gaps in our understanding drivers SWD, particularly surrounding virulence ecology. Given these uncertainties, we investigated from a novel genomic perspective by presenting first draft genome predicted proteome pathogenic isolate SR_Ha_C, generated hybrid assembly Nanopore...