A5005213567- Gut microbiota and health
- Inflammatory Bowel Disease
- Clostridium difficile and Clostridium perfringens research
- Escherichia coli research studies
- Helicobacter pylori-related gastroenterology studies
- Gastrointestinal motility and disorders
- Diet and metabolism studies
- Microscopic Colitis
- COVID-19 Impact on Reproduction
- Digestive system and related health
- Nutritional Studies and Diet
- Pelvic floor disorders treatments
- Eosinophilic Esophagitis
- Liver Diseases and Immunity
- SARS-CoV-2 detection and testing
- Anorectal Disease Treatments and Outcomes
- Probiotics and Fermented Foods
- SARS-CoV-2 and COVID-19 Research
- Celiac Disease Research and Management
- Immune Response and Inflammation
- Reproductive tract infections research
- COVID-19 Clinical Research Studies
- Mycobacterium research and diagnosis
- Pregnancy and Medication Impact
- Galectins and Cancer Biology
University of Massachusetts Chan Medical School
2012-2024
American Physiological Society
2024
RELX Group (United States)
2023
University of Puerto Rico at Río Piedras
2010-2014
University of Puerto Rico System
2010
Abstract Background P-glycoprotein (P-gp) plays a critical role in protection of the intestinal epithelia by mediating efflux drugs/xenobiotics from mucosa into gut lumen. Recent studies bring to light that P-gp also confers link communication between mucosal barrier function and innate immune system. Yet, despite knowledge for over 10 years central gastrointestinal homeostasis, precise molecular mechanism controls its functional expression regulation remains unclear. Here, we assessed how...
Diet is a modifiable, noninvasive, inexpensive behavior that crucial in shaping the intestinal microbiome. A microbiome "imbalance" or dysbiosis inflammatory bowel disease (IBD) linked to inflammation. Here, we aim define impact of specific foods on bacterial species commonly depleted patients with IBD better inform dietary treatment. We performed single-arm, pre-post intervention trial. After baseline period, IBD-Anti-Inflammatory (IBD-AID) was initiated. collected stool and blood samples...
The enteric pathogen Salmonella enterica serovar Typhimurium causes food poisoning resulting in gastroenteritis. S. effector invasion protein A (SipA) promotes gastroenteritis by functional motifs that trigger either mechanisms of inflammation or bacterial entry. During infection intestinal epithelial cells, SipA was found to be responsible for the early activation caspase-3, an enzyme is required cleavage at a specific recognition motif divided into its two domains and activated manner...
Shigella is unique among enteric pathogens, as it invades colonic epithelia through the basolateral pole. Therefore, has evolved ability to breach intestinal epithelial barrier deploy an arsenal of effector proteins, which permits bacterial invasion and leads a severe inflammatory response. However, mechanisms used by regulate permeability remain unknown. To address this question, we both polarized model human ex-vivo further characterize early events host-bacteria interactions. Our results...
ABSTRACT Helicobacter pylori is the dominant member of gastric microbiota and has been associated with an increased risk cancer peptic ulcers in adults. H. populations have migrated diverged human populations, health effects vary. Here, we describe whole genome cag -positive strain V225d, cultured from a Venezuelan Piaroa Amerindian subject. To gain insight into evolution host adaptation this bacterium, undertook comparative genomic analyses. A robust multiprotein phylogenetic tree reflects...
Crohn's disease (CD), a type of inflammatory bowel (IBD), is chronic condition the gastrointestinal tract that caused by loss mucosal tolerance towards commensal bacteria resulting in responses. It has long been postulated gut microbiota, complex and dynamic population microorganisms, plays key role pathogenesis IBD. Maternal diagnosis IBD identified as greatest risk factor for offspring increasing odds developing >4.5-fold. Moreover, babies born to mothers with have demonstrated reduced...
ABSTRACT The reason for the striking differences in clinical outcomes of SARS-CoV-2 infected patients is still poorly understood. While most recover, a subset people become critically ill and succumb to disease. Thus, identification biomarkers that can predict COVID-19 disease key help prioritize needing urgent treatment. Given an unbalanced gut microbiome reflection poor health, we aim identify indicator species could outcomes. Here, first time with largest patient cohort reported studies,...
Abstract Background Caribbean Latino adults are at high risk for osteoporosis yet remain underrepresented in bone research. This increased is attributed to genetics, diet, and lifestyle known drive inflammation microbial dysbiosis. Objective The primary objective of this study was determine whether consuming 5 oz yogurt daily 8wks improves turnover markers (BTMs) among > 50 years; secondarily the impact on gut microbiota intestinal integrity inflammation. Methods Following a 4wk baseline...
SARS-CoV-2-positive patients exhibit gut and oral microbiome dysbiosis, which is associated with various aspects of COVID-19 disease (1-4). Here, we aim to identify markers that predict severity in hospitalized patients, specifically severely ill compared moderately ones. Moreover, investigate whether hospital feeding (solid versus enteral), an important cofounder, influences the microbial composition patients. We used random forest classification machine learning models interpretable...
Crohn's disease (CD) is a chronic immune-mediated inflammatory condition caused by the loss of mucosal tolerance toward commensal microbiota. On average, 29.5% and 42.7% CD patients experience perianal complications at 10 20 y after diagnosis, respectively. Perianal (pCD) result in high burden, diminished quality life, elevated health-care costs. Overall pCD are predictors poor long-term outcomes. Animal models gut inflammation have failed to fully recapitulate human manifestations...
The American Heart Association (AHA) dietary guidelines recommend 30–35% of energy intake (%E) be from total fat, <7%E saturated fatty acids (SFA), and <1%E trans acid (TFA). This study evaluates the effect AHA counselling on fat intake. Between 2009 2014, 119 obese adults with metabolic syndrome (MetS), (71% women, average 52.5 years age, 34.9 kg/m2 body mass index), received individual group diet, over a one-year period. Each participant attended 2 sessions (months 1 12) 12 sessions,...
Shigella spp. are highly adapted pathogens that cause bacillary dysentery in human and nonhuman primates. An unusual feature of pathogenesis is this organism invades the colonic epithelia from basolateral pole. Therefore, it has evolved ability to disrupt intestinal epithelial barrier reach surface. We have shown previously secreted serine protease A (SepA), which belongs family autotransporters Enterobacteriaceae, responsible for initial destabilization facilitates invasion. However,...
Abstract Background Infants receive their first bacteria from birthing parent. This newly acquired microbiome plays a pivotal role in developing robust immune system, the cornerstone of long-term health. Results We demonstrated that gut, vaginal, and oral microbial diversity pregnant women with SARS-CoV-2 infection is reduced, early infections exhibit different vaginal microbiota composition at time delivery compared to healthy control counterparts. Accordingly, low relative abundance two...
Pregnancy is a vulnerable time where the lives of mother and baby are affected by diet, especially high-risk pregnancies in women with inflammatory bowel disease (IBD). Limited research has examined diet during pregnancy IBD.Describe compare quality pregnant without IBD, examine associations between dietary intake guidelines pregnancy.Three 24 h recalls were utilized to assess diets IBD (n = 88) 82) 27-29 weeks gestation. A customized frequency questionnaire was also administered measure...
Abstract Background Helicobacter pylori has diverged in parallel to its human host, leading distinct phylogeographic populations. Recent evidence suggests that the current mixing Latin America, European H. (hpEurope) are increasingly dominant at expense of Amerindian haplotypes (hspAmerind). This phenomenon might occur via DNA recombination, modulated by restriction-modification systems (RMS), which differences cognate recognition sites (CRS) and active methylases will determine direction...
Diet has been increasingly shown to be of therapeutic benefit for patients with inflammatory bowel diseases (IBD), especially Crohn's disease (CD). Yet dietary guidelines are nonexistent. Moreover, diets tailored Puerto Ricans IBD living on the island, have not developed and tested. The rising prevalence in Rico warrants exploring use diet as part treatment strategies these [1]. Here, we describe study design "Dieta Anti-Inflamatoria" or DAIN, a parallel two-arm randomized pilot trial aiming...
Abstract Background Evaluating the performance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serological assays and clearly articulating utility selected antigens, isotypes, thresholds is crucial to understanding prevalence infection within communities. Methods This cross-sectional study, implemented in 2020, screened PCR–confirmed disease 2019 patients (n = 86), banked prepandemic negative samples 96), healthcare workers family members 552), university employees 327) for...
Abstract Background and aims Vaginal dysbiosis has been associated with adverse pregnancy outcomes. Here, we characterized the vaginal microbiota of pregnant individuals inflammatory bowel disease (IBD) investigated whether gut or inflammation diet influence diversity these individuals. Study Design We recruited 48 in their third trimester (IBD=23 HC=18). by 16S rRNA sequencing shotgun sequencing. measured fecal calprotectin stool pro-inflammatory cytokines fluids. determine dietary quality...