Doxorubicin (DOX) is a widely used first-line antitumor agent; however, acquired drug resistance and side effects have become the main challenges to effective cancer therapy. Herein, DOX loaded into iron-rich metal-organic framework/tannic acid (TA) nanocomplex form tumor-targeting acid-activatable delivery system (MOF/TA-DOX, MTD). Under acidic tumor microenvironment, MTD simultaneously releases ferrous ion (Fe2+) accompanied by degradation. Apart from chemotherapeutic effect, elevates...

Imaging-guided percutaneous microwave thermotherapy has been regarded as an important alternative nonsurgical therapeutic strategy for hepatocellular carcinoma (HCC) that provides excellent local tumor control and favorable survival benefit. However, providing a high-resolution, real-time, noninvasive imaging technique intraoperative guidance controlling postoperative residual recurrence are urgent needs the clinical setting. In this study, cisplatin (CDDP)-loaded nanocapsule (NPs@CDDP) with...

Abstract Photodynamic therapy (PDT) is extensively investigated for tumor in the clinic. However, efficacy of PDT severely limited by tissue penetrability light, short effective half‐life and radius reactive oxygen species (ROS), weak immunostimulatory effect. In this study, a glutathione (GSH)‐activatable nano‐photosensitizer developed to load with arachidonic acid (AA) camouflage erythrocyte membrane, which serves as laser‐ignited lipid peroxidation nanoamplifier (MAR). The photosensitive...

Impeded by the limited light penetration of photodynamic therapy (PDT) to tissues and hypoxic environment solid tumors, clinical therapeutic efficacy application are below expectations. In this study, a glutathione (GSH)-responsive nano-photosensitizer, based on chlorquinaldol (CQD)-loaded iron-containing nanorod composed meso-tetra (4-carboxyphenyl) porphyrin (TCPP), was prepared serve as laser-ignited ferroptosis sensitizer improve tumoricidal effect PDT. tumor microenvironment (TME) with...

The most common type of kidney tumor, clear-cell renal cell carcinoma (ccRCC) with relatively insidious development and easily metastatic characteristics is generally insensitive to cytotoxic chemotherapy. abundant polyunsaturated fatty acids (PUFAs) content in advanced ccRCC allows it be intrinsically vulnerable ferroptosis-based therapeutic strategies. Nevertheless, the strategy cause "iron overload" by administration iron-based nanomaterials has limited efficacy. And classic ferroptosis...

Aims: Our previous studies have demonstrated that as a mitochondria-targeting cancer phototherapy, high-fluence, low-power laser irradiation (HF-LPLI) results in oxidative damage induces tumor cell apoptosis. In this study, we focused on the immunological effects of HF-LPLI phototherapy and explored its antitumor immune regulatory mechanism. Results: We found not only treatment induced apoptosis but also HF-LPLI-treated apoptotic cells activated macrophages. Due to mitochondrial superoxide...

Photodynamic therapy (PDT) is a promising alternative and palliative therapeutic strategy for colorectal cancer (CRC). A novel photosensitizer with higher selectivity CRC fewer side effects vital clinical application. Given that the overexpression of hydrogen sulfide (H2S) in CRC, it expected to provide selective stimulus activatable photosensitizers respond specific microenvironment. Herein, we report development metal-organic frameworks (MOFs) composed meso-Tetra (4-carboxyphenyl) porphine...

Purpose: To investigate the mechanisms by which PD98059 and LY294002 interfere with abnormal deposition of extracellular matrix regulated connective tissue growth factor (CTGF) rat pulmonary artery smooth muscle cells (PASMCs). Methods: Rat PASMCs were cultured separated into a control group. Real-time fluorescence quantitative PCR was performed to detect expression collagen III fibronectin mRNA. Immunohistochemistry western blot analyses protein. Results: The mRNA greater in stimulated CTGF...

Retinoblastoma (RB) is the most common primary intraocular malignancy of infancy. An alternative RB treatment protocol proposed and tested. It based on a photodynamic therapy (PDT) with designed molecular beacon that specifically targets murine double minute x (MDMX) high-expressed cells.A MDMX mRNA triggered by binding photosensitizer molecule (pyropheophorbide-a, or PPa) black hole quencher-3 (BHQ3) through complementary oligonucleotide sequence. Cells without high-expression are incubated...

Background WWOX and FHIT are two candidate tumor suppressor genes located in active fragile sites, the damage of which has been associated with development breast cancer. The association expression these cancer not fully explored. We evaluated mRNA protein tissue normal histological appearances, atypical ductal hyperplasia, carcinoma situ, invasive to see if a progressive decline was present. Methods Reverse transcription-polymerase chain reaction Western blotting were used evaluate...

Our previous studies have demonstrated that as a mitochondria-targeting cancer phototherapy, high fluence low-power laser irradiation (HF-LPLI) induces mitochondrial superoxide anion burst, resulting in oxidative damage to tumor cells. In this study, we further explored the immunological effects of HF-LPLI-induced apoptotic When macrophages were co-incubated with cells induced by HF-LPLI, observed increased levels TNF-α secretion and NO production macrophages. Further experiments showed...

Phagocytosis and subsequent degradation of pathogens by macrophages play a pivotal role in host innate immunity in mammals. Laser irradiation has been found to produce photobiological effects with evidence interference with immunological functions. However, the laser on immune response have not extensively characterized. In this study, we focused our attention He-Ne phagocytic activity using flow cytometry (FCM). After irradiating at fluence 0, 1, 2 J/cm (632.8 nm, 3mw), cells...

Abstract BackgroundThe most common type of kidney tumor, clear-cell renal cell carcinoma (ccRCC) with relatively insidious development and easily metastatic characteristics is generally insensitive to cytotoxic chemotherapy. Advanced ccRCC lacks effective treatment has a poor prognosis. Fortunately, the hallmark abundant polyunsaturated fatty acids (PUFAs) content regarded as intrinsically vulnerable ferroptosis-based therapeutic strategies. Nonetheless, classic ferroptosis agonist (RSL3)...

Objective To explore the effects of integrin ανβ5 and extracellular regulated protein kinase (ERK1/2) on matrix deposition pulmonary artery smooth muscle cells (PASMC) induced by connective tissue growth factor (CTGF) in vitro, to investigate mechanisms CTGF-integrin signal pathway vascular remodeling hypertension. Methods (1) PASMC SD rat were cultured cell purity was evaluated α-SMA immunohistochemical staining. The experiment divided into control group, CTGF group (CTGF 50 ng/ml),...

Objective To explore the mechanisms of integrin β1 on connective tissue growth factor(CTGF) -induced proliferation, migration, change cytoskeleton pulmonary arterial smooth muscle cell(PASMC) in vitro, and to investigate effects CTGF-integrin signal pathway vascular remodeling hypertension(PAH). Methods Pulmonary artery cells SD rats were cultured vitro.WST-1 assay was used detect anti-integrin antibody CTGF-induced proliferation PASMC.Transwell chambers observe migration PASMC.The...

High fluence low-power laser irradiation (HF-LPLI) provides a new stimulator to trigger cell apoptosis, and it is well known that apoptotic cells provide antigens effectively recognition by the immune system. In order investigate effect of HF-LPLI on professional antigen-presenting (APC) function, in our primary study, we focused attention HF-LPLI-treated tumor macrophages phagocytosis NO production. Both confocal microscopy flowcytometry analysis showed (120 J/cm²) induced...

Many cardiovascular diseases, such as pulmonary hypertension, atherosclerotic plaque formation and restenosis after angioplasty, are charaterized by vascular remodeling resulting from migration proliferation of smooth muscle cells. Integin receptors can activate the expression related genes through corresponding signal pathway, lead to cell' s other biological behavioral change, which may be a new hotspot pathogenesis intervention remodeling. Key words: Integrin;  Vascular cell; ...

Lipopolysaccharide (LPS), a structural component of the outer membrane gram negative bacteria, has been suggested that stimulates macrophages secrete wide variety inflammatory mediators, such as nitric oxide (NO). However, cellular mechanisms NO generation in macrophage by LPS stimulation are not well known. In this study, stimulated was determined measuring fluorescence changes with specific probe DAF-FM DA. Using resonance energy transfer (FRET) techniques, we found an increase protein...