Expression quantitative trait locus (eQTL) analysis, which links variations in gene expression to genotypes, is essential understanding regulation and interpreting disease-associated loci. Currently identified eQTLs are mainly samples of blood other normal tissues. However, no database comprehensively provides large number cancer samples. Using the genotype data 9196 tumor 33 types from The Cancer Genome Atlas (TCGA), we 5 606 570 eQTL-gene pairs cis-eQTL analysis 231 210 trans-eQTL...

Long non-coding RNAs (lncRNAs) have been identified as critical players in tumorigenesis. Previous studies revealed that lncRNA SBF2-AS1 was involved tumor progression. However, the role and underlying mechanism of cervical cancer (CC) remain unknown. In present study, our data showed expression significantly increased CC. High associated with advanced FIGO stage lymph node metastasis CC patients. Function assays inhibition reduced cells proliferation both vitro vivo. Mechanistically, we...

LSH, a SNF2 family DNA helicase, is key regulator of methylation in mammals. How LSH facilitates not well defined. While previous studies with mouse embryonic stem cells (mESc) and fibroblasts (MEFs) derived from Lsh knockout mice have revealed role de novo by Dnmt3a/3b, here we report that contributes to various cell lines primarily promoting DNMT1. We show loss has much bigger effect than DNMT3A DNMT3B. Mechanistically, demonstrate interacts UHRF1 but DNMT1 chromatin association...

Abstract Histone demethylase KDM7A regulates neuronal differentiation and development in mammals. In this study, we found that was also required for breast cancer stem cells (BCSCs) maintenance. Silencing significantly reduced the BCSCs population mamosphere formation vitro, inhibited tumor growth vivo. Restoring expression rescued defect cell Our mechanism analysis suggested upregulated stemness‐associated factors KLF4 c‐MYC addition, knockdown promoted apoptosis through decreasing BCL2 BAD...

Abstract Grazing causes major disturbances to grassland ecosystems and may alter soil enzyme activity. However, the mechanism by which grazing intensity, livestock type, duration environmental (e.g., climate soil) factors affect response of enzymes is currently unknown. This meta‐analysis investigated underlying intrinsic mechanisms based on 443 observations collected from 38 studies. Overall, had a negative effect activity, especially activities β‐glucosidase, sucrase, urease alkaline...

Impeded by the limited light penetration of photodynamic therapy (PDT) to tissues and hypoxic environment solid tumors, clinical therapeutic efficacy application are below expectations. In this study, a glutathione (GSH)-responsive nano-photosensitizer, based on chlorquinaldol (CQD)-loaded iron-containing nanorod composed meso-tetra (4-carboxyphenyl) porphyrin (TCPP), was prepared serve as laser-ignited ferroptosis sensitizer improve tumoricidal effect PDT. tumor microenvironment (TME) with...

CpG island methylator phenotype (CIMP) involves methylation targeted toward the promoters of multiple genes. We determined a profile tumor-related genes in serum sporadic breast cancer (SBC). The multigene was examined by methylation-specific polymerase chain reaction assay 50 SBCs and paired nontumors, CIMP+ defined as having three that are concordantly methylated. frequency ten varied from 10% FHIT to 74% RASSF1A. status RASSF1A, BRCA1, p16, CDH1, ER, RARβ2, APC, DAPK significantly...

H3K4me3 and H3K36me2 histone demethylase KDM2B is an epigenetic regulatory factor involved in cell proliferation numerous cells including breast cancer cells, however, the mechanism of specifically triple negative (TNBC), remains largely unknown. In this study, we showed that higher expression level was associated with poor prognosis TNBC. Using assay, found promoted TNBC by suppressing transcription cycle inhibitors p15INK4B, p16INK4A, p57KIP2. Chromatin immunoprecipitation assay results...

We investigated the hypermethylation status in serum of sporadic breast cancer patients. Material and Methods: The BRCA1, p16 14-3-3s cancerous tissues paired 38 patients was examined by methylation-specific PCR (MSP) assay. Normal benign tissue control DNA were also to determine specificity hypermethylation. Results: Hypermethylation 1 or more genes found 36/38 (95%) cancers. BRCA1 hypermethylated 14/38 (37%), 13/38 (34%), 14- 3-3s 33/38 (87%) tissues. 71% corresponding positive for...

Objective: We aimed to evaluate the association between polycyclic aromatic hydrocarbons (PAHs)-related microRNAs (miRNAs) and heart rate variability indices in coke oven workers. Methods: recruited 365 male workers measured urinary PAH metabolites by gas chromatography–mass spectrometry. Five were using three-channel Holter monitor. Six miRNAs detected TaqMan miRNA assays (Life Technologies, Foster City, CA). Results: miR-24-3p, miR-27a-3p, miR-142-5p, miR-320b negatively associated with...

Vegetation dynamics are an important topic in the field of global environment change, which is great significance to monitor temporal–spatial variability desertification at regional or scales. Following reported reversion late 1990s Horqin Sandy Land, issue was concerned for control by decreased water availability. To detect process, MODIS Normalized Difference Index (NDVI) sequences were investigated analyze effect on vegetation over 2000–2015 growing season. Results showed that: (1) NDVI...

Triple-negative breast cancer (TNBC) has a more invasive and metastatic potential than the other types of hence is associated with poor prognosis. Zeste homolog 2 (EZH2) DNA methyltransferase 1 (DNMT1) could lead to tumorigenesis by separately methylating histone H3K27 CpG islands in tumor suppressor genes. In order investigate association between oncogenesis distribution single nucleotide polymorphisms (SNPs) EZH2, DNMT1, case–control study on SNPs TNBC cases from south China was conducted....

Abstract Single nucleotide polymorphisms (SNPs) within microRNAs (miRNAs) and their target binding sites can influence miRNA biogenesis regulation, thereby participating in a variety of diseases biological processes. Current miRNA-related SNP databases are often species-limited or based on outdated data. Therefore, we updated our miRNASNP database to version 4 by updating data, expanding the species from Homo sapiens 17 species, introducing several new features. In miRNASNP-v4, 82 580 SNPs...

Abstract Cervical cancer is one of the most common gynecologic cancers around world. Long noncoding RNA s (lnc s) are considered to be important regulators some biological processes. Recently, it has been reported that linc‐ UFC 1 a putative oncogene in cancers. However, functional roles have not investigated cervical cancer. Here, was demonstrated expression significantly increased tissues, and its overexpression associated with poor survival patients Loss‐of‐function assays indicated...

Ovarian cancer (OC) is the most lethal gynecological malignancy and one of leading causes cancer-related deaths among women. Metastasis main cause poor prognosis in OC. MicroRNA (miRNA/miR) has been shown to play an important role tumorigenesis metastasis various types by affecting expression its targets. In present study, miR-32 (miR-32-5p) OC was explored. Reverse transcription-quantitative PCR results showed that significantly upregulated both tissues cell lines. Inhibition transfection...

<i>Objective:</i> DNA methylation is a common molecular alteration in human neoplasia and can be detected easily the bloodstream of patients. Here, we investigated whether sera prognostic significance breast cancer <i>Methods:</i> Methylation status BRCA1, p16 14-3-3σ was examined by methylation-specific PCR assay sporadic patients healthy serum controls. <i>Results:</i> The panel gene frequencies were 29% cancers for p16, 32% BRCA1 82% 14-3-3σ; all...

More and more studies have reported that dysregulation of microRNAs (miRNAs) leads to the proliferation EMT multiple cancers. Recently, several reports demonstrated miR-4262 occurs in numerous However, its role precise mechanism human cervical cancer (CC) not been well clarified. Hence, this study aimed explore biological roles mechanisms CC cell lines. The level was found be significantly decreased tissues Moreover, expression closely related increased Kaiso (ZBTB33), which belongs BTB/POZ...

BACKGROUNDS: Upregulation of lncRNA AB073614 is found in some cancer types and involved tumor development progression including ovarian cancer. However, the clinical value functional role epithelial (EOC) still needed to be investigated. METHODS: We e xamined expression using quantitative real time polymerase chain reaction (qRT-PCR) 75 paired EOC tissue samples adjacent normal tissues. Association with overall survival (OS) was evaluated Kaplan-Meier analysis. Univariate multivariate...

ABSTRACT The feasibility of using digital chroma measurements to determine the protein content in rice was investigated red, green and blue (RGB) values reflect color change. samples ranged from 5 g/100 g 13 g. There a significant negative linear correlation ( r < − 0.98) between relevant solutions treated with biuret reagent. Of all tricolor values, G value optimal parameter difference solutions. It had higher coefficient 0.99) more sensitive subtle differences than R or B values. With...

Mounting evidence indicates that long non-coding RNAs influence the progression of cervical cancer, but precise function LINC01503 in pathogenesis disease remains unknown.Here, we found higher levels cancer tissues.High expression was associated with enhanced as indicated by advanced FIGO stage, increased metastasis tumor cells to lymph nodes, and invasion into deeper tissues.LINC01503 inhibition markedly suppressed proliferative ability cells.Mechanistically, demonstrated negatively...

Ovarian cancer is a prevalent female malignancy affecting the health and life of an increasing population women around world. Paclitaxel (PTX) resistance significant clinical problem in treatment ovarian cancer. However, regulation mechanism PTX remains unclear. In this investigation, we reported innovative function long noncoding RNA RMRP promoting glycolysis cells. We observed that was highly expressed samples, which expression elevated PTX-resistant patients compared with PTX-sensitive...

Mutants on stalk strength are essential materials for the studies formation of plant cell wall. In this study, a brittle mutant maize, designated as Bk-x, was screened from Mutator inserted library. At germination and early seedling stage, plants were indistinguishable normal ones. However, all organs after 5th-leaf stage remained throughout rest growing period. Microstructure observation showed that wall in vascular bundle sheath Bk-x thinner than plants. The leaf mechanical 77.9% at...

Breast cancer is one of the most common malignancies in women. ERα, PR, and HER2 triple negative breast (TNBC) current research focus because lack effective targeted therapies. In our study, lentivirus systems were used to overexpress EZH2 miR-1301 TNBC cell lines. Western blot analysis RT-qPCR detect protein microRNA levels. The TCGA Kaplan Meier plotter databases analyze expression levels cancer. effect overexpression on proliferation, migration colony formation determined by using...