A5101811156- Acute Myeloid Leukemia Research
- Acute Lymphoblastic Leukemia research
- Hematopoietic Stem Cell Transplantation
- CAR-T cell therapy research
- Epigenetics and DNA Methylation
- Childhood Cancer Survivors' Quality of Life
- Virus-based gene therapy research
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Neutropenia and Cancer Infections
- RNA modifications and cancer
- Cytomegalovirus and herpesvirus research
- Ubiquitin and proteasome pathways
- Neuroblastoma Research and Treatments
- Histone Deacetylase Inhibitors Research
- Polyomavirus and related diseases
- Ovarian cancer diagnosis and treatment
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Chronic Myeloid Leukemia Treatments
- Retinoids in leukemia and cellular processes
- Cancer-related Molecular Pathways
- Immune Cell Function and Interaction
- Hematological disorders and diagnostics
Soochow University
2016-2025
China Medical University
2025
First Hospital of China Medical University
2025
Soochow University
2014-2024
Children's Hospital of Suzhou University
2022-2023
Shandong University
2022-2023
Yale University
2022-2023
University of Tulsa
2022-2023
Shandong Provincial Hospital
2022-2023
Yunnan University of Finance And Economics
2023
We determined the safety and efficacy of coadministration CD19- CD22-chimeric antigen receptor (CAR) T cells in patients with refractory disease or high-risk hematologic isolated extramedullary relapse B-acute lymphoblastic leukemia.
Introduction Corticosteroids are used for toxicity management, raising concerns about whether they may affect the anti-leukemic effects of chimeric antigen receptor (CAR)-T cells. Methods and results In this study, we retrospectively analyzed patients (fined two subgroups based on disease burden. Of 75 cases in low burden (LDB) group (MRD < 5%, no extramedullary disease), there was significant difference between use steroids event-free survival (EFS) ( p = 0.21) overall (OS) 0.26),...
Abstract Background Survivin, a member of the family inhibitor apoptosis proteins, functions as key regulator mitosis and programmed cell death. YM155, novel molecular targeted agent, suppresses survivin, which is overexpressed in many tumor types. The aim this study was to determine antitumor activity YM155 SK-NEP-1 cells. Methods growth vitro vivo assessed by MTT nude mice experiments. Annexin V/propidium iodide staining followed flow cytometric analysis used detect culture. Then gene...
Acute myeloid leukemia (AML) is the second most common form of in children. Aberrant DNA methylation patterns are a characteristic feature various tumors, including AML. Metallothionein III (MT3) tumor suppresser reported to show promoter hypermethylated cancers. However, expression and molecular function MT3 pediatric AML unclear. Eleven human cell lines 41 samples 20 NBM/ITP (Norma bone marrow/Idiopathic thrombocytopenic purpura) control were analyzed. Transcription levels evaluated by...
CpG island methylator phenotype (CIMP) involves methylation targeted toward the promoters of multiple genes. We determined a profile tumor-related genes in serum sporadic breast cancer (SBC). The multigene was examined by methylation-specific polymerase chain reaction assay 50 SBCs and paired nontumors, CIMP+ defined as having three that are concordantly methylated. frequency ten varied from 10% FHIT to 74% RASSF1A. status RASSF1A, BRCA1, p16, CDH1, ER, RARβ2, APC, DAPK significantly...
Abstract Background There is growing evidence supporting a role for microRNAs (miRNA) as targets in aberrant mechanisms of DNA hypermethylation. Epigenetic silencing tumor suppressor miRNAs, including miR-663, which has recently been reported to be inactivated by hypermethylation several cancers, may play important roles pediatric acute myeloid leukemia (AML). However, expression miR-663 and its promoter methylation remain status unclear childhood leukemia. Methods Promoter was investigated...
The purpose of this study is to report the first nationwide protocol (Wuhan Protocol) developed by Chinese Children's Cancer Group and results multidisciplinary effort in treating hepatoblastoma. In study, we reported final analysis, which includes 153 hepatoblastoma patients 13 hospitals from January 2006 December 2013. 6-year overall survival event-free rates were 83.3 ± 3.1% 71.0 3.7%, respectively, cohort. univariate analysis revealed that female (P = 0.027), under 5 years age 0.039),...
Decreased autophagy is accompanied by the development of a myeloproliferative state or acute myeloid leukemia (AML). AML cells are often sensitive to autophagy‑inducing stimuli, prompting idea that targeting can be useful in cytotoxic therapy. NB4 overexpressing microtubule-associated protein 1 light chain 3-green fluorescent were screened with 69 inhibitors analyze activity. treated polo-like kinase (PLK1) RO3280 and BI2536 before analysis. Cleaved LC3 (LC3-II) phosphorylation mammalian...
Overexpression of cyclin D1 dependent kinases 4 and 6 (CDK4/6) is a common feature many human cancers including leukemia. LEE011 novel inhibitor both CDK4 6. To date, the molecular function in leukemia remains unclear.Leukemia cell growth apoptosis following treatment was assessed through CCK-8 annexin V/propidium iodide staining assays. Cell senescence by β-galactosidase p16INK4a expression analysis. Gene profiles treated HL-60 cells were investigated using an Arraystar Human LncRNA array....
Pediatric acute myeloid leukemia (AML) comprises up to 20% of all childhood leukemia. Recent research shows that aberrant DNA methylation patterning may play a role in leukemogenesis. The epigenetic silencing the EBF3 locus is very frequent glioblastoma. However, expression profiles and molecular function pediatric AML still unclear.Twelve human cell lines, 105 samples 30 normal bone marrow/idiopathic thrombocytopenic purpura (NBM/ITP) control were analyzed. Transcriptional level was...
Both hepatitis B virus (HBV) and gene methylation play important roles in hepatocarcinogenesis. However, their association between HBV infection is not fully understood. Cell cycle control involving RB1 gene-related cell inhibitors one of the main regulatory pathways were reported to be altered hepatocellular carcinoma (HCC). The purpose this research assess status p14 (ARF) INK4 family (p14 , p15 (INK4B) p16 (INK4A) p18 (INK4C) ) HCC with without it, discuss possible role HBV-induced...
The objective of this study was to observe the prognosis pediatric patients with E2A-PBX1-positive acute lymphoblastic leukemia (ALL) from treatment CCLG-ALL2008 protocol.Three hundred and forty-nine Chinese pre-B-cell ALL were enrolled in December 2008 September 2013. Of these, 20 E2A-PBX1 expression 223 without gene stratified into two cohorts. Clinical biological characteristics 5-year event-free survival (EFS), relapse-free (RFS), overall (OS) analyzed compared between these groups.The...
CD19-targeted chimeric antigen receptor T-cell (CAR-T) therapy has shown remarkable efficacy in treating relapsed or refractory pediatric B-lineage acute lymphoblastic leukemia (B-ALL). However, poor results are obtained when the same product is reused patients who relapse after CAR-T. Therefore, there a need to explore safety and of co-administration CD19- CD22-targeted CAR-T as salvage second (CART2) B-ALL their first CD19 treatment (CART1).In this study, we recruited five CD22-CAR...
Polo-like kinase 1 (PLK1) is highly expressed in many cancers and therefore a biomarker of transformation potential target for the development cancer-specific small molecule drugs. RO3280 was recently identified as novel PLK1 inhibitor; however its therapeutic effects leukemia treatment are still unknown. We found that protein cell lines well 73.3% (11/15) pediatric acute myeloid (AML) samples. mRNA expression significantly higher AML samples compared with control (82.95 ± 110.28 vs. 6.36...
Autoimmune hemolytic anemia (AIHA) is a rare disease in children, and its clinical severity varies. To better understand manifestation treatment outcome, we analyzed 68 children diagnosed as AIHA for characteristics, laboratory findings, outcomes. Data show that primary accounted 39.7% of all patients, whereas secondary 60.3%. Among them, Evans syndrome (ES) 20 cases (29.4%). Average hemoglobin was lower the 1-year or below age group than above group, combined-antibody single-antibody...
Abstract Background Acute myeloid leukemia (AML) is a malignancy of the hematopoietic system, and childhood AML accounts for about 20% pediatric leukemia. ANP32B, an important nuclear protein associated with proliferation, has been found to regulate hematopoiesis CML leukemogenesis by inhibiting p53 activity. However, recent study suggests that ANP32B exerts suppressive effect on B-cell acute lymphoblastic (ALL) in mice activating PU.1. Nevertheless, precise underlying mechanism remains...
The Real-time PCR Array System is the ideal tool for analyzing expression of a focused panel genes. In this study, we will analyze gene profile pediatric acute myeloid leukemia with real-time arrays.Real-time array was designed and tested firstly. Then 11 AML 10 normal controls analyzed arrays. We data MEV (Multi Experiment View) cluster software. Datasets representing genes altered derived from analyses were imported into Ingenuity Pathway Analysis Tool.We 88 primer pairs quantitative...
Background Wilms tumor (WT) is an embryonic kidney cancer, for which histone acetylation might be a therapeutic target. LBH589, novel targeted agent, suppresses deacetylases in many tumors. This study investigated the antitumor activity of LBH589 SK-NEP-1 and G401 cells. Methods cell growth was assessed by CCK-8 nude mice experiments. Annexin V/propidium iodide staining followed flow cytometry detected apoptosis culture. Gene expressions LBH589-treated cells were analyzed using Arraystar...
A growing body of evidence indicates that long non-coding RNA (lncRNA) is involved in the development and progression many diseases. It has been reported lncRNA LINC00467 disregulated multiple tumors, while its role acute myeloid leukemia (AML) still unknown. Here, we find expression significantly increased AML specimens cell lines. Further investigations show knockdown inhibits malignant phenotypes cells. Consistently, slows immunodeficient mice. Interestingly, microRNA-339 (miR-339)...
Histone modification enzymes regulate gene expression by altering the accessibility of promoters to transcription factors. We sought determine whether genes encoding histone are dysregulated in pediatric acute lymphoblastic leukemia (ALL). A real-time PCR array was designed, tested and used profile 85 bone marrow mononuclear cells from 30 ALL patients 20 normal controls. The histone-modifying significantly different between karyotype B cell Eleven were upregulated ALL, including deacetylases...
Early detection and prediction of prognosis treatment responses are all the keys in improving survival ovarian cancer patients. This study profiled an progression model to identify prognostic biomarkers for Mouse surface epithelial cells (MOSECs) can undergo spontaneous malignant transformation vitro cell culture. These were used as a alterations gene expression signaling detected using Illumina HiSeq2000 Next-Generation Sequencing platform bioinformatical analyses. The differential four...