A5100655512- Reproductive Biology and Fertility
- Assisted Reproductive Technology and Twin Pregnancy
- Ovarian function and disorders
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Sperm and Testicular Function
- Reproductive System and Pregnancy
- RNA Research and Splicing
- MicroRNA in disease regulation
- Reproductive Health and Technologies
- Autophagy in Disease and Therapy
- Nuclear Structure and Function
- Calcium signaling and nucleotide metabolism
- Animal Genetics and Reproduction
- Cancer-related gene regulation
- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- RNA modifications and cancer
- Sirtuins and Resveratrol in Medicine
- Genomics and Chromatin Dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Pregnancy and preeclampsia studies
- Pluripotent Stem Cells Research
- Sexual Differentiation and Disorders
Nanjing Maternity and Child Health Care Hospital
2015-2025
Nanjing Medical University
2015-2025
Anhui Medical University
2016-2023
First Affiliated Hospital of Anhui Medical University
2020-2023
Ministry of Education of the People's Republic of China
2023
Yuhuangding Hospital
2022
Shandong University
2022
University of Science and Technology of China
2022
Suzhou Municipal Hospital
2020
Anhui Provincial Hospital
2017-2020
To study the antitumor effect of matrine in human hepatoma G2 (HepG2) cells and its molecular mechanism involved antineoplastic activities.3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to detect viability HepG2 cells. The on cell cycle detected by fl ow cytometry. Annexin-V-FITC/PI double staining cellular apoptosis. Cellular morphological changes were observed under an inverted phase contrast microscope. Transmission electron microscopy performed further...
Background Male infertility is a major issue of human reproduction health. Asthenoteratospermia can impair sperm motility and cause male infertility. with multiple morphological abnormalities the flagella (MMAF) presents abnormal spermatozoa absent, bent, coiled, short and/or irregular-calibre flagella. Previous studies on MMAF reported that genetic defects in cilia-related genes (eg, AKAP4 , DNAH1 CFAP43 CFAP44 CFAP69 ) are MMAF. However, known MMAF-associated only responsible for...
Background Asthenoteratospermia, one of the most common causes for male infertility, often presents with defective sperm heads and/or flagella. Multiple morphological abnormalities flagella (MMAF) is clinical manifestations asthenoteratospermia. Variants in several genes including DNAH1 , CEP135 CATSPER2 and SUN5 are involved genetic pathogenesis However, more than half asthenoteratospermia cases cannot be explained by known pathogenic genes. Methods results Two asthenoteratospermia-affected...
Abstract Purpose To elucidate the characterization of extracellular vesicles (EVs) in follicular fluid-derived (FF-EVs) and discover critical molecules signaling pathways associating with etiology pathobiology PCOS, differentially expressed miRNAs (DEmiRNAs) proteins profiles (DEPs) were initially explored combinedly analyzed. Methods First, miRNA protein expression FF-EVs PCOS patients control compared by RNA-sequencing tandem mass tagging (TMT) proteomic methods. Subsequently, Gene...
Abstract Resveratrol is a polyphenol derivatives which exhibits pro-apoptotic effect in variety of human cancers by triggering mitochondria apoptosis pathway and autophagy. However, there are scarcely reports on its apoptosis-promoting abnormal proliferation fibroblast-like synoviocytes (FLSs). In this study, we investigated the underlying mechanism apoptosis-inducing effects resveratrol FLSs adjuvant-arthritis (AA) rats. Since using for 12 days resulted significant decreasing swelling...
Abstract The human zona pellucida (ZP) is a highly organized glycoprotein matrix that encircles oocytes and plays an essential role in successful reproduction. Previous studies have reported mutations ZP1, ZP2 ZP3 influence their functions result lack of ZP or abnormal empty follicle syndrome, which leads to female infertility. Here, we performed whole‐exome sequencing two probands with primary infertility whose lacked ZP, identified heterozygous mutation ZP1 (NM_207341:c.326G>A...
Abstract Background Potassium channels are important for the structure and function of spermatozoa. As a potassium transporter, mSlo3 is essential male fertility as Slo3 knockout mice were infertile with series functional defects in sperm cells. However, no pathogenic variant has been detected human SLO3 to date. Here we reported case homozygous mutation. The corresponding assisted reproductive strategy also investigated. Methods We performed whole-exome sequencing analysis from large cohort...
Microplastics (MPs) have been identified in various human tissues and organs. This study aims to evaluate the presence of MPs follicular fluid (hFF) their potential impact on oocyte maturation. Laser direct infrared spectroscopy 19 hFF samples 7956 particles, including 1739 microplastics (21.9 %) from 30 types. Of these, 923 particles had matching degrees > 0.8, 7033 between 0.65 0.8. The most abundant were Chlorinated Polyethylene (CPE), Fluorosilicone rubber, Polyvinyl chloride (PVC),...
PAT1 homolog 2 (PATL2), encoding an RNA-binding protein, is a repressor involved in the translational regulation of maternal mRNAs during oocyte maturation. Previous studies have reported mutations PATL2 those led to female infertility with maturation arrest; however, mechanisms by which affected meiotic remained unclear. Here, we identified several novel and recurrent patients similar phenotype, chose missense mutation c.649 T>A p.Tyr217Asn (PATL2 Y217N ) as typical investigate...
MicroRNA-29b (miR29b) has been previously identified in early mouse embryos through miRNA microarray analysis. Recent research indicated that miR29b participates DNA methylation by regulating methyltransferase 3a/3b (Dnmt3a/3b) expression. However, the expression pattern and biological function of preimplantation embryonic development remain unknown.In this study, we examined patterns Dnmt3a/3b at different developmental stages. Subsequently, localization DNMT3A/3B protein was analyzed...
Abstract In order to evaluate the impact of assisted reproductive technology (ART) procedure and individual factors on sex ratio singletons twins at birth after in vitro fertilization (IVF) intra-cytoplasmic sperm injection (ICSI) treatment China. We conducted a retrospective cohort study including patients undergoing their first cycle IVF or ICSI with autologous oocytes from 2001 2015. A total 7410 babies were born 5832 women live birth. The secondary (SSR) was significantly higher than...
Polycystic ovarian syndrome (PCOS) causes a significantly increased risk of hyperstimulation (OHSS). Here, we focused on the altered expression serum miRNAs and their predictive value for OHSS in PCOS patients.We used TaqMan low density array followed by individual quantitative reverse transcription-polymerase chain reaction to identify validate patients likely develop severe OHSS.The miR-16 miR-223 levels were reduced who than control subjects mild or no OHSS. The sensitivity specificity...
We conducted this study for the purpose of evaluating protective mechanisms curcumin against oxidative stress in asthenozoospermic individuals. Asthenozoospermic individuals were grouped into AS group, treatment group and brusatol + group. The sperm motility was measured by computer-aided analysis. flow cytometry spectrophotometry to assess levels reactive oxygen species (ROS) malondialdehyde (MDA). Chlortetracycline (CTC) used examine acrosomal reaction spermatozoa. Also, Western blotting...
To clarify the role of activated Notch2 in invasiveness gastric cancer.To investigate silencing gene expression, we established a small interfering RNA (siRNA) transfected cell line using MKN-45 cancer line. After successful transfection confirmed by real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, migration invasion assays were employed to evaluate aggressiveness cancer. RT-PCR blottings confirm down-regulation expression epithelial mesenchymal...