A5000378702- Sarcoidosis and Beryllium Toxicity Research
- Sepsis Diagnosis and Treatment
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Respiratory Support and Mechanisms
- Neonatal Respiratory Health Research
- Immune Response and Inflammation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Intensive Care Unit Cognitive Disorders
- Pulmonary Hypertension Research and Treatments
- Heme Oxygenase-1 and Carbon Monoxide
- Thermal Regulation in Medicine
- Mitochondrial Function and Pathology
- Eicosanoids and Hypertension Pharmacology
- S100 Proteins and Annexins
- Hemoglobinopathies and Related Disorders
- Tuberculosis Research and Epidemiology
- Climate Change and Health Impacts
- Effects of Radiation Exposure
- RNA modifications and cancer
- Sirtuins and Resveratrol in Medicine
- ATP Synthase and ATPases Research
- Research on Leishmaniasis Studies
- Inflammasome and immune disorders
- Peptidase Inhibition and Analysis
- Macrophage Migration Inhibitory Factor
University of Florida
2024-2025
Jupiter (United States)
2025
University of Arizona
2015-2024
Scripps Research Institute
2024
Evanston Hospital
2022
ORCID
2021
RELX Group (Netherlands)
2020
University of Illinois Chicago
2013-2014
Boston University
2014
Howard University
2014
Sarcoidosis is a granulomatous lung disorder of unknown cause. The majority individuals with sarcoidosis spontaneously achieve full remission (uncomplicated sarcoidosis), however, ~20% sarcoidosis-affected experience progressive disease or cardiac and nervous system involvement (complicated sarcoidosis). We investigated peripheral blood mononuclear cell (PBMC) microRNA protein-coding gene expression data from healthy controls patients uncomplicated complicated sarcoidosis. identified 46...
Abstract Background There is a compelling unmet medical need for biomarker-based models to risk-stratify patients with acute respiratory distress syndrome. Effective stratification would optimize participant selection clinical trial enrollment by focusing on those most likely benefit from new interventions. Our objective was develop prognostic, model predicting mortality in adult Methods This secondary analysis using cohort of 252 mechanically ventilated subjects the diagnosis Survival day 7...
We previously demonstrated involvement of NAMPT (nicotinamide phosphoribosyltransferase) in pulmonary arterial hypertension (PAH) and now examine regulation extracellular NAMPT’s (eNAMPT’s) role PAH vascular remodeling. transcription protein expression human lung endothelial cells were assessed response to PAH-relevant stimuli (PDGF [platelet-derived growth factor], VEGF [vascular TGF-β1 [transforming factor-β1], hypoxia). Endothelial-to-mesenchymal transition was detected by SNAI1 (snail...
Abstract Despite encouraging preclinical data, therapies to reduce ARDS mortality remains a globally unmet need, including during the COVID-19 pandemic. We previously identified extracellular nicotinamide phosphoribosyltransferase (eNAMPT) as novel damage-associated molecular pattern protein (DAMP) via TLR4 ligation which regulates inflammatory cascade activation. eNAMPT is tightly linked human by biomarker and genotyping studies in subjects. now hypothesize that an eNAMPT-neutralizing mAb...
Abstract Although the progression of non‐alcoholic fatty liver disease (NAFLD) from steatosis to steatohepatitis (NASH) and cirrhosis remains poorly understood, a critical role for dysregulated innate immunity has emerged. We examined utility ALT‐100, monoclonal antibody (mAb), in reducing NAFLD severity NASH/hepatic fibrosis. ALT‐100 neutralizes eNAMPT (extracellular nicotinamide phosphoribosyltransferase), novel damage‐associated molecular pattern protein (DAMP) Toll‐like receptor 4 (TLR4)...
Genetic factors are involved in acute respiratory distress syndrome (ARDS) susceptibility. Identification of novel candidate genes associated with increased risk and severity will improve our understanding ARDS pathophysiology enhance efforts to develop preventive therapeutic approaches.
Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal disorder without curative therapies, underscoring the critical unmet need for identification of novel therapeutics. Extracellular nicotinamide phosphoribosyltransferase (eNAMPT) damage-associated molecular pattern protein (DAMP) Toll-Like Receptor 4 (TLR4) ligand that contributes to severity radiation-induced lung NASH-associated hepatic fibrosis. This study investigates eNAMPT as druggable target in human preclinical IPF...
BackgroundThere remains a serious need to prevent the progression of invasive prostate cancer (PCa). We previously showed that secreted extracellular nicotinamide phosphoribosyltransferase (eNAMPT) is multifunctional innate immunity regulator via TLR4 ligation which has been implicated in PCa progression. Here we investigate role eNAMPT as diagnostic biomarker and therapeutic target PCa.MethodsTumor NAMPT expression plasma level were evaluated human subjects with various tumor stages high...
The paucity of therapeutic strategies to reduce the severity radiation-induced lung fibrosis (RILF), a life-threatening complication intended or accidental ionizing radiation exposure, is serious unmet need. We evaluated contribution eNAMPT (extracellular nicotinamide phosphoribosyltransferase), damage-associated molecular pattern (DAMP) protein and TLR4 (Toll-like receptor 4) ligand, whole-thorax irradiation (WTLI)-induced RILF. Wild-type (WT) Nampt+/- heterozygous C57BL6 mice nonhuman...
We postulated that the hypoxic response in sickle cell disease (SCD) contributes to altered gene expression and pulmonary hypertension, a complication associated with early mortality.To identify genes regulated by not other effects of chronic anemia, we compared variation peripheral blood mononuclear cells from 13 subjects SCD hemoglobin SS genotype 15 Chuvash polycythemia (VHL(R200W) homozygotes constitutive upregulation hypoxia-inducible factors absence anemia or hypoxia). At 5% false...
Background: Numerous potential ARDS therapeutics, based upon preclinical successful rodent studies that utilized LPS challenge without mechanical ventilation, have failed in Phase 2/3 clinical trials. Recently, ALT-100 mAb, a novel biologic neutralizes the TLR4 ligand and DAMP, eNAMPT (extracellular nicotinamide phosphoribosyltransferase), was shown to reduce septic shock/VILI-induced porcine lung injury when delivered 2 h after onset. We now examine mAb efficacy on acute kidney (AKI) fluid...
Rationale Circadian systems drive the expression of multiple genes in nearly all cells and coordinate cellular-, tissue-, system-level processes that are critical to innate immunity regulation. Objective We examined effects circadian rhythm disorganization, produced by light shift exposure, on immunity-mediated inflammatory lung responses including vascular permeability gene a C57BL/6J murine model injury. Methods A total 32 mice were assigned phase shifting (CPS) with intratracheal...
Pharmacologic interventions to halt/reverse the vascular remodeling and right ventricular dysfunction in pulmonary arterial hypertension (PAH) remains an unmet need. We previously demonstrated extracellular nicotinamide phosphoribosyltransferase (eNAMPT) as a DAMP (damage‐associated molecular pattern protein) contributing PAH pathobiology via TLR4 ligation. examined role of endothelial cell (EC)‐specific eNAMPT experimental PH eNAMPT‐neutralizing mAb therapeutic strategy reverse established...
Abstract Rationale Despite the availability of multi-“omics” strategies, insights into etiology and pathogenesis sarcoidosis have been elusive. This is partly due to lack reliable preclinical models a paucity validated biomarkers. As granulomas are key feature sarcoidosis, we speculate that direct genomic interrogation sarcoid tissues, may lead identification dysregulated gene pathways or biomarker signatures. Objective To facilitate development biomarkers by expression profiling in lung...
Abstract Background Nicotinamide phosphoribosyltransferase (NAMPT) exhibits dual functionality – as an intracellular enzyme regulating nicotinamide adenine dinucleotide metabolism and extracellular secreted protein (eNAMPT) to function a cytokine regulator of innate immunity via binding Toll-Like receptor 4 NF-κB activation. In limited preclinical clinical studies, eNAMPT was implicated in the pathobiology acute respiratory distress syndrome (ARDS) suggesting that could potentially serve...
Effective therapies to reduce the severity and high mortality of pulmonary vasculitis diffuse alveolar hemorrhage (DAH) in patients with systemic lupus erythematosus (SLE) is a serious unmet need. We explored whether biologic neutralization eNAMPT (extracellular nicotinamide phosphoribosyl-transferase), novel DAMP Toll-like receptor 4 ligand, represents viable therapeutic strategy vasculitis.
<h3>Background</h3> Sex-based differences are important in the development and progression of pulmonary arterial hypertension. However, it is not established whether these generalizable to all forms hypertension (PH). <h3>Research Question</h3> What sex-based right ventricle (RV) function transplant-free survival patients with PH from Redefining Pulmonary Hypertension Through Vascular Disease Phenomics (PVDOMICS) cohort? <h3>Study Design Methods</h3> Patients enrolled PVDOMICS cohort study...
Acute respiratory distress syndrome (ARDS) is an inflammatory process of the lungs that develops primarily in response to pulmonary or systemic sepsis, resulting a disproportionate death toll intensive care units (ICUs). Given its role as critical activator and innate immune responses, previous studies have reported increase circulating cell-free mitochondrial DNA (mtDNA) biomarker for fatal outcome ICU. Here we analyzed association whole-blood mtDNA (wb-mtDNA) copies with 28-day survival...
Abstract Compelling preclinical studies indicate that low-dose carbon monoxide (CO) abrogates experimental lung fibrosis. We recently reported the results of a multicenter, double-blinded, clinical trial inhaled CO in patients with idiopathic pulmonary fibrosis (IPF). Identifying no significantly changes metalloproteinase-7 (MMP7) serum concentration, or secondary endpoints physiologic measurements, hospitalization, death, patient-reported outcomes. In present study, we evaluated effect low...
Introduction: Intra-amniotic inflammation (IAI) or chorioamnionitis is a common complication of pregnancy producing significant maternal morbidity/mortality, premature birth and neonatal risk chronic lung diseases such as bronchopulmonary dysplasia (BPD). We examined eNAMPT (extracellular nicotinamide phosphoribosyltransferase), critical inflammatory DAMP TLR4 ligand, potential therapeutic target to reduce IAI severity improve adverse fetal/neonatal outcomes. Methods: Blood/tissue samples...