A5101568652- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Gut microbiota and health
- Bacteriophages and microbial interactions
- Microbial Metabolism and Applications
- Demographic Trends and Gender Preferences
- Microbial Natural Products and Biosynthesis
- Probiotics and Fermented Foods
- Bacterial biofilms and quorum sensing
- Clostridium difficile and Clostridium perfringens research
- Chromium effects and bioremediation
- Plant Gene Expression Analysis
- Essential Oils and Antimicrobial Activity
- Immunotherapy and Immune Responses
- Antimicrobial Peptides and Activities
- Cervical Cancer and HPV Research
- Bee Products Chemical Analysis
- Psoriasis: Treatment and Pathogenesis
- Advancements in Transdermal Drug Delivery
- Circular RNAs in diseases
- Identification and Quantification in Food
- Biochemical and Structural Characterization
- Biochemical and biochemical processes
- HIV, TB, and STIs Epidemiology
- Cancer Immunotherapy and Biomarkers
Chongqing Public Health Medical Center
2021-2025
Chongqing Medical University
2021-2025
University of Cambridge
2025
Sichuan University
2012-2024
Shanghai Institute of Materia Medica
2024
Chinese Academy of Sciences
2024
Kunming University of Science and Technology
2024
Jilin University
2024
Chengdu Institute of Biology
2024
Beijing Municipal Ecological and Environmental Monitoring Center
2024
Colon cancer (CC) is a common malignant cancer. Recently, circFNDC3B was found to exert biological function in multiple cancers. However, it unclear whether the potential protein encoded by involved carcinogenesis of CC.We used Sanger sequence and RNase R digestion assay confirm existence circFNDC3B, quantitative real-time PCR evaluate circRNA's expression. Then fluorescence situ hybridization (FISH) performed study location circFNDC3B. The identification using LC-MS/MS. circFNDC3B-218aa on...
Intracellular bacteria have been shown to cause autophagy, which impacts infectious outcomes, whereas extracellular not reported activate autophagy. Here, we demonstrate that Pseudomonas aeruginosa, a Gram-negative bacterium, activates autophagy with considerably increased LC3 punctation in both an alveolar macrophage cell line (MH-S) and primary macrophages. Using the Gly120 mutant, successfully demonstrated hallmark of conjugation phosphatidylethanolamine (PE). The accumulation typical...
Pseudomonas aeruginosa is known to generate bacterial biofilms that increase antibiotic resistance. With the of multi-drug resistance in recent years, formulation a new therapeutic strategy has seemed urgent. Preliminary findings show Prodigiosin (PG), derived from chromium-resistant Serratia marcescens, exhibited efficient anti-biofilm activity against Staphylococcus aureus. However, its P. remains largely unexplored. The PG three clinical single drug-resistant was evaluated using crystal...
Abstract Gut bacteria from the Enterobacteriaceae family are a major cause of opportunistic infections worldwide. Given their prevalence among healthy human gut microbiomes, interspecies interactions may play role in modulating infection resistance. Here we uncover global ecological patterns linked to colonization and abundance by leveraging large-scale dataset 12,238 public metagenomes spanning 45 countries. Machine learning analyses identified robust microbiome signature associated with...
Surface seawater in the South Pacific Gyre (SPG) is one of cleanest oceanic environments on earth, and photosynthetic primary production extremely low. Despite ecological significance largest aquatic desert our planet, microbial community composition ultra-oligotrophic remain largely unknown. In this study, we collected surface along a southern transect SPG during Integrated Ocean Drilling Program (IODP) Expedition 329. Samples from four distinct sites (Sites U1368, U1369, U1370 U1371) were...
The rise of antibiotic resistance poses a significant public health crisis, particularly due to limited antimicrobial options for the treatment infections with Gram-negative pathogens. Here, an peptide (AMP) SR25 is characterized, which effectively kills both and Gram-positive bacteria through unique dual-targeting mechanism without detectable resistance. Meanwhile, SR25-functionalized hydrogel developed efficient infected diabetic wounds. obtained genome mining from uncultured bovine...
Patchouli alcohol (PA) is a widely used pharmaceutical ingredient in various Chinese traditional herbal medicine (THM) formulations, known for its modulatory effects on the gut microbiota. The present study investigated PA's anti-inflammatory and regulatory microbiota mode of action (MOA). Based assessments ulcerative colitis (UC) symptoms, PA exhibited promising preventions against inflammatory response. In accordance, expressions pro-inflammatory factors, including interleukin (IL)-1β,...
The WRKY family is one of the largest transcription factor (TF) families in plants and plays central roles modulating plant stress responses developmental processes, as well secondary metabolic regulations. Lotus (Nelumbo nucifera) an aquatic crop that has significant food, ornamental pharmacological values. Here, we performed overview analysis TF members lotus, studied their functions environmental adaptation regulation lotus benzylisoquinoline alkaloid (BIA) biosynthesis. A total 65 genes...
Bacterial biofilm can cause nosocomial recurrent infections and implanted device secondary in patients strongly promotes development of pathogenic drug resistance clinical treatments. Butenolide is an effective anti-macrofouling compound derived from a marine Streptomyces sp., but its antibiofilm efficacy remains largely unexplored. In the present study, activities butenolide were examined using biofilms formed by both Gram-positive Gram-negative model species. Four Escherichia coli strains,...
A novel Gram-stain-negative, rod-shaped, non-flagellated, strictly aerobic strain with gliding motility, designated SW024 T , was isolated from surface seawater of the South Pacific Gyre (26° 29′ S 137° 56′ W) during Integrated Ocean Drilling Program Expedition 329. Phylogenetic analyses based on 16S rRNA gene sequences showed that related most closely to Aquimarina muelleri KMM 6020 macrocephali JAMB N27 addita JC2680 mytili PSC33 intermedia 6258 latercula ATCC 23177 spongiae A6 agarilytica...
Antimicrobial peptides (AMPs) provide a promising strategy against infections involving multidrug resistant pathogens. In previous studies, we designed short 12 amino acid AMP DP7, using machine-learning method based on activity contribution matrix. DP7 shows broad-spectrum antimicrobial activities both in vitro and vivo. Here, aim to investigate the efficacy of Staphylococcus aureus (S. aureus) reveal potential mechanisms. First, by measuring killing kinetics S. comparing these results with...
Antimicrobial peptides are promising alternative antimicrobial agents to combat MDR. DP7, an peptide designed in silico, possesses broad-spectrum activities and immunomodulatory effects. However, the effects of DP7 against Pseudomonas aeruginosa biofilm infection remain largely unexplored.To assess (i) activity MDR P. aeruginosa; (ii) antibiofilm infection. Also, preliminarily investigate possible mode action.The MICs for 104 clinical strains (including 57 strains) were determined. RNA-Seq,...
Prevention and control of infections have become a formidable challenge due to the increasing resistance pathogens antibiotics. Probiotics been discovered positive effects on host, it is well-known that some Lactobacilli are effective in treating preventing inflammatory infectious diseases. In this study, we developed an antibacterial formulation consisting honey Lactobacillus plantarum (honey-L. plantarum). The optimal (10%) L. (1 × 109 CFU/mL) was used investigate its antimicrobial effect...
An aerobic, Gram-stain-negative, rod-shaped and non-motile strain (XY-359T) was isolated from the mouth of a marine invertebrate Onchidium species South China Sea. It grew at pH 6.0-8.5 (optimum, 7.5), 15-37 °C 30 °C) in presence 0.5-4.5 % (w/v) NaCl 2.5 %). could not hydrolyse Tweens 20, 40, 60 or 80 no flexirubin-type pigments were produced. The major polar lipids phosphatidylethanolamine, one unidentified aminolipid, six phospholipids two lipids. fatty acids iso-C17:0 3-OH, iso-C15:1 G...
Rare Actinobacteria, known as non-Streptomyces, hold great potential to produce new bioactive compounds for drug development. A strain designated DSW09T, which belongs those rare was isolated from surface seawater of the East China Sea. The cells were aerobic, Gram-positive, non-motile, non-spore-forming and rod-shaped (0.4 µm wide 1.5-4.0 long). closest relative Euzebya tangerina F10T (96.46 % 16S rRNA gene similarity). Cell growth occurred at 15-45 °C (optimum, 25-30 °C), pH 6.0-9.0 (pH...
To develop a neoantigen-targeted personalized cancer treatment for non-small cell lung (NSCLC), neoantigens were obtained from collected human samples, and the utility of neoantigen neoantigen-reactive T cells (NRTs) was assessed.Tumor specimens three patients with NSCLC analyzed by whole-exome sequencing, predicted accordingly. Dendritic lymphocytes isolated, NRTs elicited IFN-γ ELISPOT tests conducted. HLA-A2.1/Kb transgenic mice immunized peptides HLA-A*02:01+patient high immunogenicity,...
Mutation-specific T-cell response to epithelial cancers and T-cell-based immunotherapy has been successfully used treat several human solid cancers. We aimed investigate the anti-tumour effect of neo-antigen-reactive T(NRT) cells induced by RNA mutanome vaccine, which may serve as a feasible effective therapeutic approach for lung cancer.We predicted candidate neo-antigens according mutant gene analysis sequencing mouse Lewis C57BL/6 tail tissue. vaccine was prepared with template. assessed...
A Gram-negative, rod-shaped and motile bacterium, designated strain J95T, was isolated from the rhizosphere soil of a mangrove plant Kandeliacandel (L.) Druce in Mai Po Nature Reserve, Hong Kong. Growth J95T observed at pH 5.0-8.5 (optimum, 6.0-7.0), between 10-40 °C (30-37 °C) presence 0-9 % (w/v) NaCl (0.5-3 %). Chemotaxonomic analysis showed ubiquinone-10 as predominant respiratory quinone C18 : 1ω7c C19 0 cycloω8c major fatty acids. The polar lipids were lipid, aminolipid, phospholipid,...