A5051036548- Osteoarthritis Treatment and Mechanisms
- Periodontal Regeneration and Treatments
- Knee injuries and reconstruction techniques
- Mesenchymal stem cell research
- Orthopaedic implants and arthroplasty
- Cell Adhesion Molecules Research
- Total Knee Arthroplasty Outcomes
- Hip and Femur Fractures
- Silk-based biomaterials and applications
- Orthopedic Infections and Treatments
- Rheumatoid Arthritis Research and Therapies
- Tendon Structure and Treatment
- Bone and Joint Diseases
- Cytokine Signaling Pathways and Interactions
- Lower Extremity Biomechanics and Pathologies
- Hip disorders and treatments
- Melanoma and MAPK Pathways
- Extracellular vesicles in disease
- Chemokine receptors and signaling
- Autophagy in Disease and Therapy
- Adipokines, Inflammation, and Metabolic Diseases
- NF-κB Signaling Pathways
- Inflammatory mediators and NSAID effects
- Microplastics and Plastic Pollution
- Pharmacological Effects of Natural Compounds
Nanjing Medical University
2013-2024
Jiangsu Province Hospital
2011-2023
Tokyo Medical and Dental University
2020
Zero to Three
2014
Health First
2012-2013
University of Akron
2012
Osteoarthritis is a chronic degenerative disease that can lead to restricted activity or even disability. Bone marrow mesenchymal stem cells repair cartilage damage and treat osteoarthritis via cell therapies in-tissue engineering. Research has shown the paracrine mechanism main mode of action cells. Exosomes are smallest known membrane-bound nanovesicles. also important carriers delivery agents promote communication between We demonstrated bone cell-derived exosomes delay progression...
Exogenous microparticles including microplastics are novel pollutants that could persist in the environment with potential health effects, while crucial data on their exposure humans still lacking. To understand panorama of and distribution characteristics different kinds body fluids. A non-targeted microparticle internal landscape analysis was done thirteen human enclosed fluids covering eight systems. Totally 104 patients aged 24–96 years an average age 56 were included this study. After...
Chondrocytes and bone marrow mesenchymal stem cells (BMSCs) are frequently used as seed in cartilage tissue engineering. In the present study, we determined if co-culture of rabbit articular chondrocytes BMSCs vitro promotes expression cartilaginous extracellular matrix and, so, what is optimal ratio two cell types. Cultures were expanded then cultured individually or at a chondrocyte:BMSC 4:1, 2:1, 1:1, 1:2, 1:4 for 21 days DMEM/F12. chondrogenic induction medium. Quantitative real-time...
Chondrocytes and bone marrow mesenchymal stem cells (BMSCs) are frequently used as seed in cartilage tissue engineering. In the present study, we determined if co-culture of rabbit articular chondrocytes BMSCs vitro promotes expression cartilaginous extracellular matrix and, so, what is optimal ratio two cell types. Cultures were expanded then cultured individually or at a chondrocyte:BMSC 4:1, 2:1, 1:1, 1:2, 1:4 for 21 days DMEM/F12. chondrogenic induction medium. Quantitative real-time...
Chondrocyte apoptosis is closely related to the development and progression of osteoarthritis. Global adiponectin (gAPN), secreted from adipose tissue, possesses potent anti-inflammatory antiapoptotic properties in various cell types. This study aimed investigate role autophagy induced by gAPN suppression H2O2-induced potential mechanism gAPN-induced chondrocytes.H2O2 was used induce apoptotic injury rat chondrocytes. CCK-8 assay performed determine viability cells treated with different...
Background/Aims: The biological effects of periodic mechanical stress on chondrocytes have been studied extensively over the past few years. However, mechanisms underlying chondrocyte mechanosensing and signaling in response to remain be determined. In current study, we examined focal adhesion kinase (FAK) stress-induced proliferation matrix synthesis. Methods Results: Periodic significantly induced sustained phosphorylation FAK at Tyr397 Tyr576/577. Reduction with targeted shRNA via...
ABSTRACT To evaluate the effects of intraarticular injections autologous platelet‐rich plasma (PRP) or bone marrow concentrate (BMC) on osteoarthritis (OA), 24 adult goats were equally divided into control (Ctrl), saline (NS), PRP, and BMC groups, OA was induced by surgery in NS, groups. Autologous PRP obtained from whole blood aspirates, respectively. The data revealed, platelets increased 1.8‐fold, monocytes 5.6‐fold, TGF‐β1 7.7‐fold, IGF‐1 3.6‐fold ( p < 0.05), 2.9‐fold, 3.3‐fold...
In the present study, effects and mechanisms of mesenchymal stem cells (MSCs) on interleukin (IL)-1β‑stimulated rat chondrocytes, as well cartilage from a model osteoarthritis (OA) induced by anterior cruciate ligament transection medial meniscectomy were investigated. Confluent chondrocytes treated with IL-1β (10 ng/ml), then cultured indirectly or without MSCs at ratio 2:1. Total RNA protein collected various time-points, western blot reverse transcription-quantitative polymerase chain...
Chondrocyte degeneration and classically activated macrophage (AM)-related inflammation play critical roles in osteoarthritis (OA). Here, we explored the effects of astaxanthin Rspo2 on OA vitro vivo. We observed that gene was markedly elevated synovial tissues patients compared with healthy controls. In 2D cultures, inflammatory factors were enhanced AMs nonactivated macrophages (NMs), protein expression levels Rspo2, β-catenin, increased, anabolic markers reduced osteoarthritic...
Background Recent studies have shown that the acetabular component frequently becomes deformed during press-fit insertion. The aim of this study was to explore deformation Durom cup after implantation and clarify impact on wear ion release large head metal-on-metal (MOM) total hips in simulators. Methods Six cups impacted into reamed acetabula fresh cadavers were used as experimental group another 6 size-paired intact constituted control group. All 12 MOM put through a 3 million cycle (MC)...
The mitogenic effects of periodic mechanical stress on chondrocytes have been studied extensively but the mechanisms whereby sense and respond to remain a matter debate. We explored signal transduction pathways chondrocyte proliferation matrix synthesis under stress. In particular, we sought identify role MEK1/2-ERK1/2 signaling pathway in following cyclic physiologic compression. Under stress, both rat were significantly increased (P < 0.05) associated with increases phosphorylation Src,...
The effects of periodic mechanical stress on the mitogenesis chondrocytes have been studied extensively in recent years. However, mechanisms underlying ability to sense and respond remain a matter debate. We explored signal transduction pathways proliferation matrix synthesis when were exposed stress. observed that statistically significantly enhanced phosphorylation activation Rac1 (p<0.05 for each). Pre-treatment with selective inhibitor NSC23766 attenuated stress-induced chondrocyte each)...
Periodic mechanical stress can promote chondrocyte proliferation and matrix synthesis to improve the quality of tissue-engineered cartilage. Although integrin β1-ERK1/2 signal cascade has been implicated in periodic stress-induced mitogenic effects chondrocytes, precise mechanisms have not fully established. The current study was designed probe roles CaMKII Pyk2 signaling stress-mediated synthesis.Chondrocytes were subjected stress, assessed by direct cell counting CCK-8 assay; gene...
Background/Aims: Periodic mechanical stress activates integrin β1-initiated signal pathways to promote chondrocyte proliferation and matrix synthesis. Integrin β1 overexpression has been demonstrated play important roles in improving the activities functions of several non-chondrocytic cell types. Therefore, current study, we evaluated effects up-regulation on periodic stress-induced proliferation, synthesis ERK1/2 phosphorylation monolayer culture, quality tissue-engineered cartilage...
The potential empty spaces between cylindrical plugs remaining after autologous osteochondral mosaicplasty rely on fibrous repair, which may constrain the quality and integrity of repair. Thus, should be repaired, how to fill is still a problem. In present study, standardized full-thickness defect (diameter, 6 mm) was created in weight-bearing area each medial femoral condyle both knees 18 miniature pigs. 36 were randomly assigned four groups with nine group. defects initially repaired by...
Abstract In recent years, a variety of studies have been performed to investigate the cellular responses periodic mechanical stress on chondrocytes. Integrin β1‐mediated ERK1/2 activation was proven be indispensable in stress‐induced chondrocyte proliferation and matrix synthesis. However, other signal proteins responsible for mitogenesis chondrocytes under remain incompletely understood. current investigation, we probed roles integrin‐linked kinase (ILK) signaling We found that upon...
A. sinensis polysaccharide (ASP), one of the effective components sinensis, has been used to treat inflammatory diseases in China. However, its effect on rheumatoid arthritis (RA) is unknown. The purpose this study was explore anti-inflammatory effects and mechanisms ASP RA using a rat model collagen-induced (CIA). For evaluation therapeutic vitro, CIA used. Our showed that (100 200 μg/mL) dose-dependently inhibited tumor necrosis factor (TNF)-α-induced (10 ng/mL) proliferation, migration,...