A5072302662- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CRISPR and Genetic Engineering
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- Endoplasmic Reticulum Stress and Disease
- Vascular Tumors and Angiosarcomas
- Synthesis of Tetrazole Derivatives
- Cancer, Lipids, and Metabolism
- Iron Metabolism and Disorders
- Immunotherapy and Immune Responses
- HER2/EGFR in Cancer Research
- Histone Deacetylase Inhibitors Research
- Autophagy in Disease and Therapy
- Chronic Myeloid Leukemia Treatments
- T-cell and Retrovirus Studies
- Immune cells in cancer
Inserm
2020-2024
Université Côte d'Azur
2020-2024
La Ligue Contre le Cancer
2022-2024
Centre Méditerranéen de Médecine Moléculaire
2022-2023
Laboratoire de Chimie de Coordination
2022
The success and limitations of current immunotherapies have pushed research toward the development alternative approaches possibility to manipulate other cytotoxic immune cells such as natural killer (NK) cells. Here, we targeted an intracellular inhibiting protein 'cytokine inducible SH2-containing protein' (CISH) in NK evaluate impact on their functions antitumor properties.To further understand CISH cells, developed a conditional Cish-deficient mouse model (Cishfl/flNcr1Ki/+ ). cytokine...
Programmable nucleases have enabled rapid and accessible genome engineering in eukaryotic cells living organisms. However, their delivery into human blood can be challenging. Here, we utilized "nanoblades," a new technology that delivers genomic cleaving agent cells. These are modified murine leukemia virus (MLV) or HIV-derived virus-like particle (VLP), which the viral structural protein Gag has been fused to Cas9. VLPs thus loaded with Cas9 complexed guide RNAs. Highly efficient gene...
Genome engineering has become more accessible thanks to the CRISPR-Cas9 gene-editing system. However, using this technology in synthetic organs called "organoids" is still very inefficient. This due delivery methods for machinery, which include electroporation of DNA, mRNA, or ribonucleoproteins containing Cas9-gRNA complex. these procedures are quite toxic organoids. Here, we describe use "nanoblade (NB)" technology, outperformed by far levels achieved date murine- and human tissue-derived...
Abstract Background Angioimmunoblastic T-cell lymphoma (AITL) is a malignancy with very poor survival outcome, in urgent need of more specific therapeutic strategies. The drivers this disease are CD4 + follicular helper T cells (Tfh). metabolism these malignant Tfh was not yet elucidated. Therefore, we decided to identify their metabolic requirements the objective propose novel option. Methods To reveal prominent pathways used by AITL cells, relied on metabolomic and proteomic analysis...
IRE1α is one of the three ER transmembrane transducers Unfolded Protein Response (UPR) activated under endoplasmic reticulum (ER) stress. activation has a dual role in cancer as it may be either pro- or anti-tumoral depending on studied models. Here, we describe discovery that exogenous expression IRE1α, resulting auto-activation, did not affect cell proliferation vitro but resulted tumor-suppressive phenotype syngeneic immunocompetent mice. We found murine colorectal and Lewis lung...
For angioimmunoblastic T cell lymphoma (AITL), a rare cancer, no specific treatments are available and survival outcome is poor. We previously developed murine model for AITL that mimics closely human disease allows to evaluate new treatments. As in AITL, the CD4
In CML, Leukemic Stem Cells (LSCs) that are insensitive to Tyrosine Kinase Inhibitors responsible for leukemia maintenance and relapses upon TKI treatment arrest. We previously showed downregulation of the BMI1 polycomb protein is crucial stem/progenitor cells self-renewal induced a CCNG2/dependent proliferation arrest leading elimination Chronic Myeloid Leukemia (CML) cells. Unfortunately, as today, pharmacological inhibition has not made its way clinic.We used Connectivity Map...
Abstract Cancer metabolic reprogramming has been recognized as one of the cancer hallmarks that promote cell proliferation, survival, well therapeutic resistance. Up-to-date regulation metabolism in T-cell lymphoma is poorly understood. In particular, for human angioimmunoblastic (AITL) profile not known. Metabolic intervention could help identify new treatment options this with very poor outcomes and no effective medication. Transcriptomic analysis AITL tumor cells, identified these cells...
Abstract Cytokine inducible SH2-containing protein (CISH) is a natural killer (NK) cell negative regulator of cytokine signaling pathway. To further understand CISH functions in NK cells, we developed conditional Cish -deficient mouse model cells ( fl/fl Ncr1 Ki/+ ). We detected no developmental or homeostatic difference cells. However, global gene expression compared to +/+ revealed upregulation pathways and genes associated with cycling activation. show that does not only regulate...
Abstract Genome engineering has become more accessible thanks to the RNA programmable endonucleases such as CRISPR/Cas9 system. However, using this editing technology in synthetic organs called ‘organoids’ is still very inefficient. This due delivery methods used for CRISPR-Cas9 machinery, which include electroporation of DNA, mRNA or ribonucleoproteins (RNPs) containing CAS9-gRNA complex. these procedures are toxic some extent organoids. Here we describe use ‘Nanoblade’ technology,...