A5036443957- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Immune cells in cancer
- Quantum Dots Synthesis And Properties
- RNA Interference and Gene Delivery
- Inflammation biomarkers and pathways
- Cell Adhesion Molecules Research
- Adipokines, Inflammation, and Metabolic Diseases
- Advanced biosensing and bioanalysis techniques
- Advanced MRI Techniques and Applications
- Iron Metabolism and Disorders
- Fatty Acid Research and Health
- MRI in cancer diagnosis
- Lanthanide and Transition Metal Complexes
- Environmental and biological studies
- Nanocluster Synthesis and Applications
- Eicosanoids and Hypertension Pharmacology
- Advanced Drug Delivery Systems
- Nanoparticles: synthesis and applications
- Lysosomal Storage Disorders Research
- Advanced Nanomaterials in Catalysis
- Dye analysis and toxicity
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Carbon and Quantum Dots Applications
- Slime Mold and Myxomycetes Research
Universidade de São Paulo
2014-2023
Institute of Chemistry
2016
Gold nanoparticle (AuNP) bioconjugates have been used as therapeutic and diagnostic tools; however, in vivo biocompatibility cytotoxicity continue to be two fundamental issues. The effect of AuNPs (20 nm) conjugated with antibody [immunoglobulin G (IgG)], albumin, protein A, PEG4000, citrate (cit) were evaluated vitro using primary human cells the vascular system. AuNP did not cause lysis erythrocytes, apoptosis or necrosis leukocytes, endothelial vitro, although had internalized detected...
Keywords: cationic USPIOs, MRI, contrast agent, magnetic targeting, in vivo toxicity, intravital microscopy
Nanostructured contrast agents are promising alternatives to Gd3+-based chelates in magnetic resonance (MR) imaging techniques. A novel ultrasmall paramagnetic nanoparticle (UPN) was strategically designed maximize the number of exposed sites and r1 while minimizing r2, by decorating 3 nm titanium dioxide nanoparticles with suitable amounts iron oxide. Its relaxometric parameters comparable those gadoteric acid (GA) agar phantoms, r2/r1 ratio 1.38 at T is close ideal unitary value. The...
Abstract: Melanoma is a severe metastatic skin cancer with poor prognosis and no effective treatment. Therefore, novel therapeutic approaches using nanotechnology have been proposed to improve effectiveness. Lipid-core nanocapsules (LNCs), prepared poly(ε-caprolactone), capric/caprylic triglyceride, sorbitan monostearate stabilized by polysorbate 80, are efficient as drug delivery systems. Here, we investigated the effects of acetyleugenol-loaded LNC (AcE-LNC) on human SK-Mel-28 melanoma...
It is highly desirable in biomedical sciences to utilize the multifunctional nanoparticles of similar size with tunable emission. Since optoelectronic properties quantum dots (QDs) originate from size-dependent confinement effects, we developed an alternate approach synthesize color-tunable CdSe/ZnS QDs based on interfacial ion exchange (predominantly Se2– by S2– anions), using 1-dodecanethiol and oleylamine solvent systems as a sensitive parameter. The wide-range color-tunability (490–570...
The biocompatibility, bionanointeraction, uptake efficiency, and entry pathway of luminescent nanomaterials are the key factors to understand development an efficient bionanoprobe. foremost objective this work is explore potential 3-mercaptopropionic acid (3-MPA) capped ZnSe:xMn2+ (x = 5, 10, 15 mol %) quantum dots (QDs) for bionanoprobe used in future biological clinical applications. For purpose, highly intense orange-emitting activator Mn2+ ion doped ZnSe QDs were synthesized via a...
To develop fluorescent bionanoprobe for cellular imaging, it is crucial to get precise insight on the particle–cell interaction, optical properties of QDs in and out cell, their movement final fate particle.
Aim: Poly(ε-caprolactone) lipid-core nanocapsules (LNCs) are efficient drug carriers and drug-free LNCs display therapeutic effects, inhibiting tumor growth neutrophil activities. Herein, we investigated the direct actions of on human immune cells, to guide their application. Materials & methods: LNC's uptake, cytokine release, cell migration, proliferation intracellular pathways under inflammatory stimulation were investigated. Results conclusion: quickly penetrated leukocytes without...
Role of poly(e-caprolactone) lipid-core nanocapsules on melanoma-neutrophil crosstalk Carine C Drewes,1 Aline de CS Alves,2,3 Cristina B Hebeda,1 Isabela Copetti,2,3 Silvana Sandri,1 Mayara K Uchiyama,4 Koiti Araki,4 Silvia S Guterres,2 Adriana R Pohlmann,2,3 Sandra H Farsky1 1Department Clinical and Toxicological Analyses, School Pharmaceutical Sciences, University São Paulo, 2Postgraduate Program in 3Department Organic Chemistry, Institute Federal Rio Grande do Sul, Porto Alegre,...
Aim: To develop a series of superparamagnetic iron oxide nanoparticles (SPIONs) by coconjugating them with ibuprofen (ibu) and glycerol phosphate (glycerol) or ibu glucose-1-phosphate to assess capacity these conjugates inhibit the release nitric (NO) in macrophages, even at low concentrations. Materials & methods: The SPION were characterized their properties evaluated showing influence those ligands on colloidal stability inhibition NO-release demonstrated. cytotoxicity possible...
Atherosclerosis can be originated from the accumulation of modified cholesterol-rich lipoproteins in arterial wall. The electronegative LDL, LDL(-), plays an important role pathogenesis atherosclerosis once this lipoprotein internalized by macrophages, contributing to formation foam cells, and provoking immune-inflammatory response. Herein, we engineered a nanoformulation containing highly pure surface-functionalized nanocapsules using single-chain fragment variable (scFv) reactive LDL(-) as...
Aim: The low solubility and consequent poor bioavailability of ibuprofen (IBU) is a major drawback that can be overcome by anchoring IBU on ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs) as effective multifunctional carriers for drug delivery. Methods: USPIONs were conjugated with glycerol phosphate (USPION-GP) also co-conjugated (USPION-GP/IBU), their in vivo toxicity anti-inflammatory effects investigated. Phosphate buffer saline (control), IBU, USPION-GP USPION-GP/IBU...
Hypertensive individuals taking anti-hypertensive drugs from renin-angiotensin system inhibitors may exhibit a more severe evolution of the disease when contracting SARS-CoV-2 virus (COVID-19 disease) due to potential increases in ACE2 expression. The study investigated ACE1 and axes hydroxychloroquine lungs adipose tissue male female normotensive Wistar Kyoto (WKY) spontaneously hypertensive rats (SHRs). SHRs were treated with losartan (10 mg/kg/day) or captopril for 14 days 7 (200 drinking...
Omega 3 fatty acids, such as docosahexaenoic acid (DHA) have been widely consumed supplements to control chronic inflammation. Nanocapsules containing DHA (MLNC-DHA-a1) were developed and showed excellent stability. Thus, our objective was evaluate the effect of MLNC-DHA-a1 nanocapsules on biomarkers inflammation.Cells viability determined by flow cytometry. The uptake macrophages their polarization determined. In vivo, LDLr(-,-) mice fed a Western diet promote inflammation treated with...
Abstract Ranelate ion, the major component of an old antiosporotic drug, exhibits a unusual chemical structure encompassing thiophene ring with two carboxylic groups, cyanonitrile substituent, and nitrile diacetate group; it undergoes decarboxylation at carbon‐5 position in acidic solutions, yielding photoreactive (H5Ran) species which converts into remarkably stable blue dithiophene dye presence UV light air.
MRI is one of the best imaging techniques in clinical analysis because it non-invasive and has high resolution. Contrast agents are used to further improve these images, as they increase level soft tissue detail, differentiating regions interest. Currently, Gd-based contrast have important precautions. In this work we present a new nanoparticle based on iron oxide, with 4 nm positive (T1-weighted). Thus, effects similar gadoteric acid, reduced signal persistence partial renal excretion,...
Nanostructured contrast agents are promising alternatives to Gd(III)-based chelates in magnetic resonance (MR) imaging techniques. A novel ultrasmall paramagnetic nanoparticle (UPN) was strategically designed maximize the number of exposed sites and r1 while minimizing r2, by decorating 3 nm large titanium dioxide nanoparticles with suitable amounts iron oxide. Its relaxometric parameters comparable that gadoteric acid (GA) agar phantoms, r2/r1 ratio 1.38 at 3T is close ideal unitary value....
Blue dyes are relatively uncommon in nature, and a novel dithiophene dye (RanB) is reported this paper. This derived from an old anti-osteoporotic drug metal ion complexing agent, displaying planar molecular structure, with two sets of carboxylate, isonitrile, thiophene, iminodiacetate groups. The blue color originates strong absorption peak at 648 nm, accompanied by unusual fluorescence 555 higher energy compared to the main band. RanB forms complexes lanthanoid ions through groups serves...