A5103285124- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- Asthma and respiratory diseases
- Viral Infections and Outbreaks Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pleural and Pulmonary Diseases
- Viral Infections and Vectors
- Protein Kinase Regulation and GTPase Signaling
- Fire effects on ecosystems
- Smoking Behavior and Cessation
- CRISPR and Genetic Engineering
- Mycobacterium research and diagnosis
- Lung Cancer Diagnosis and Treatment
- Inhalation and Respiratory Drug Delivery
- Respiratory and Cough-Related Research
- Ovarian cancer diagnosis and treatment
- Adrenal and Paraganglionic Tumors
- Receptor Mechanisms and Signaling
- COVID-19 Impact on Reproduction
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Respiratory viral infections research
- Tuberculosis Research and Epidemiology
- Reproductive System and Pregnancy
Hospital Clínico Universitario Virgen de la Victoria
2012-2024
Icahn School of Medicine at Mount Sinai
2010-2024
San Sebastián University
2021-2024
Austral University of Chile
2024
Dr. A. Ramachandran's Diabetes Hospitals
2024
Hospital Universitario Virgen de las Nieves
2024
University of Concepción
2018-2021
National Institute of Allergy and Infectious Diseases
2021
National Institutes of Health
2021
Instituto Nacional del Cáncer
2000-2015
Citation Kraus TA, Sperling RS, Engel SM, Lo Y, Kellerman L, Singh T, Loubeau M, Ge Garrido JL, Rodríguez‐García Moran TM. Peripheral blood cytokine profiling during pregnancy and post‐partum periods. Am J Reprod Immunol 2010; 64: 411–426 Problem Pregnancy requires that the maternal immune system adapt to prevent rejection of fetal semi‐allograft. This immunologic adaptation may contribute pregnancy‐related alterations in disease susceptibility severity infections from viral pathogens such...
Current evidence supports the notion that amyloid beta-peptide (Abeta) plays a major role in neurotoxicity observed brain Alzheimer's disease. However, signal transduction mechanisms involved still remain unknown. In present work, we analyzed effect of protein kinase C (PKC) on some members Wnt signaling pathway and its implications for Abeta neurotoxicity. Activation PKC by phorbol 12-myristate 13-acetate protected rat hippocampal neurons from toxicity. This was accomplished inhibition...
Influenza virus infection is a major public health burden worldwide. Available vaccines include the inactivated intramuscular trivalent vaccine and, more recently, an intranasal live attenuated influenza (LAIV). The measure of successful vaccination with systemic rise in immunoglobulin G (IgG) level, but for LAIV no such correlate has been established.Seventy-nine subjects were given FluMist. Blood was collected prior to and 3 days 30 after vaccination. Nasal wash Responses measured...
In Brief OBJECTIVE: To estimate the effects of gestational age and other maternal factors on immunologic responses to influenza vaccination. METHODS: Antepartum postpartum women receiving vaccination as part routine clinical care were enrolled through four consecutive seasons (starting October 2006 January 2010). Immunologic trivalent inactivated vaccine monovalent H1N1 assessed well influencing responsiveness. Serum samples obtained at baseline 4–8 weeks postvaccination. RESULTS: Two...
Post-exposure highly potent human monoclonal antibodies target Andes hantavirus glycoprotein in a hamster cardiopulmonary syndrome model.
Here we aim to describe early mutational events across samples from publicly available SARS-CoV-2 sequences the sequence read archive and GenBank repositories. Up until 27 March 2020, downloaded 50 illumina datasets, mostly China, USA (WA State) Australia (VIC). A total of 30 datasets (60%) contain at least a single founder mutation most variants are missense (over 63%). Five-point mutations with clonal (founder) effect were found in next-generation sequencing samples. Sequencing North...
SARS-CoV-2 (COVID-19) patients who develop acute respiratory distress syndrome (ARDS) can suffer lung injury, or even death. Early identification of severe disease is essential in order to control COVID-19 and improve prognosis. Oxidative stress (OS) appears play an important role pathogenesis; we therefore conceived a study the potential discriminative ability serum biomarkers with ARDS those mild moderate (non-ARDS). 60 subjects were enrolled single-centre, prospective cohort consecutively...
The effects of the expression Na+/H+ exchanger regulatory factor-1 (NHERF1) on distribution, dynamics, and signaling properties PTH type 1 receptor (PTH1R) were studied in rat osteosarcoma cells ROS 17/2.8. NHERF1 had a dramatic effect subcellular distribution PTH1R, promoting substantial relocation to regions plasma membrane located very close proximity cytoskeletal fibers. Direct interactions with PTH1R cytoskeleton required for these effects, because they abolished by 1) mutations that...
Satisfaction with the inhaler is an important determinant of treatment adherence in patients asthma and chronic obstructive pulmonary disease (COPD). However, few studies have compared these 2 groups to identify factors associated satisfaction inhaler. To assess compare or COPD determine variables high satisfaction. A multicenter, cross-sectional study 816 (406 410 COPD) was conducted. assessed Feeling Inhaler (FSI-10) questionnaire. All participants completed Test Adherence Inhalers either...
Despite SARS-CoV-2 being a "novel" virus, early detection of anti-spike IgG in severe COVID-19 patients may be caused by the amplification humoral memory responses against seasonal coronaviruses. Here, we examine this phenomenon characterizing non-hospitalized convalescent individuals across spectrum severity. We observe that disease severity positively correlates with levels, cross-reactivity other betacoronaviruses (β-CoVs), and FcγR activation. Analysis targeting β-CoV-conserved...
The production of phosphatidic acid plays a crucial role in the activation ERK cascade. This was linked to binding phosphatidate specific polybasic site within kinase domain Raf-1. Here we show that promotes phosphorylation intact cells but does not activate Raf vitro. suppressor Ras (KSR) contains sequence homologous Direct synthetic peptides derived from sequences domains Raf-1 and KSR demonstrated by spectroscopic techniques. specificity these interactions confirmed using lipids mutated...
The fluorescent properties of the amino acid tryptophan make it a useful tool for fluorometric assays. Because fluorescence is remarkably sensitive to polarity environment, can be used determine affinity tryptophan-containing peptides phospholipid vesicles varying compositions. Here, we describe method using binding affinities derived from proteins Raf-1 and KSR-1 small unilamellar containing phosphatidic acid. extrapolated measure other or lipid vesicles.
The coronavirus disease 2019 (COVID19) pandemic has left researchers scrambling to identify the humoral immune correlates of protection from COVID-19. To date, antibody mediated virus neutralization have been extensively studied. However, extent that non-neutralizing functions contribute anti-viral responses are ill defined. In this study, we profiled anti-spike subtype/subclass responses, along with and antibody-dependent natural killer cell in 83 blood samples collected between 4 201 days...
The role of phospholipase D (PLD) in the regulation traffic PTH type 1 receptor (PTH1R) was studied Chinese hamster ovary cells stably transfected with a human PTH1R (CHO-R3) and rat osteosarcoma 17/2.8 (ROS) cells. PTH(1-34) increased total PLD activity by 3-fold CHO-R3 2-fold ROS Overexpression wild-type (WT) PLD1 WT-PLD2 basal but not Ligand-stimulated greatly WT-PLD1 WT-PLD2. However, only expression PTH-dependent Expression catalytically inactive mutants R898K-PLD1 (DN-PLD1) R758K-PLD2...
EBNA3C is an EBV-encoded nuclear protein, essential for proliferation of EBV infected B-lymphocytes. Using amino acids 365-545 in a yeast two hybrid screen, we found interaction with the Growth Arrest and DNA-damage Gadd34. When both proteins are overexpressed, Gadd34 can interact cytoplasmic compartments. Amino 483-610 Gadd34, including PP1a interaction, HSV-1 ICPγ34.5 homology domains, required interaction. Furthermore, lost mutant (509 DVIEVID 515→AVIAVIA), that abolishes coactivation...
Patients present a wide range of clinical severities in response severe acute respiratory syndrome coronavirus 2 infection, but the underlying molecular and cellular reasons why outcomes vary so greatly within population remains unknown. Here, we report that negative severely ill patients were associated with divergent RNA transcriptome profiles peripheral immune cells compared mild cases during first weeks after disease onset. Protein–protein interaction analysis indicated early-responding...
Patients present a wide range of clinical severities in response SARS-CoV-2 infection, but the underlying molecular and cellular reasons why outcomes vary so greatly within population remains unknown. Here, we report that negative severely ill patients were associated with divergent RNA transcriptome profiles peripheral immune cells compared mild cases during first weeks after disease onset. Protein-protein interaction analysis indicated early-responding cytotoxic NK an effective clearance...
Patients present a wide range of clinical severities in response SARS-CoV-2 infection, but the underlying molecular and cellular reasons why outcomes vary so greatly within population remains unknown. Here, we report that negative severely ill patients were associated with divergent RNA transcriptome profiles peripheral immune cells compared mild cases during first weeks after disease onset. Protein-protein interaction analysis indicated early-responding cytotoxic NK an effective clearance...
To describe the clinical management of asthmatic patients and level compliance with