A5101433435- Genomics and Phylogenetic Studies
- Yersinia bacterium, plague, ectoparasites research
- Actinomycetales infections and treatment
- RNA modifications and cancer
- Pharmacological Effects of Natural Compounds
- Probiotics and Fermented Foods
- Cancer-related gene regulation
- Plant Pathogenic Bacteria Studies
- Epigenetics and DNA Methylation
- Plant Disease Resistance and Genetics
- Bacillus and Francisella bacterial research
- Cancer-related molecular mechanisms research
- Genetic Syndromes and Imprinting
- Gut microbiota and health
- Cancer Research and Treatments
- Land Use and Ecosystem Services
- Urban Design and Spatial Analysis
- Infections and bacterial resistance
- Oral microbiology and periodontitis research
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- Identification and Quantification in Food
- Aquaculture disease management and microbiota
- Microbial Natural Products and Biosynthesis
- Viral Infections and Vectors
National Institute for Communicable Disease Control and Prevention
2019-2023
Gansu University of Traditional Chinese Medicine
2023
Peking University
2023
Shanghai Public Health Clinical Center
2020-2021
Ningbo University
2020
ORCID
2020
Gansu Provincial Center for Disease Control and Prevention
2010-2017
Sichuan University
2009-2014
Emerging zoonoses of wildlife origin caused by previously unknown agents are one the most important challenges for human health. The Qinghai-Tibet Plateau represents a unique ecological niche with diverse that harbours several pathogens and numerous uncharacterized pathogens. In this study, we identified characterized novel arenavirus (namely, plateau pika virus, PPV) from pikas (Ochotona curzoniae) on virome analysis. Isolated PPV strains could replicate in mammalian cells. We further...
Six novel facultatively anaerobic, Gram-stain-positive, rod-shaped, non-haemolytic bacteria (zg-320T/zg-336, zg-917T/zg-910 and zg-913T/zg-915) isolated from animal tissues human faeces were found to belong the genus Corynebacterium based on phylogenetic analyses of 16S rRNA gene 262 core genes set. Based greatest degree similarity, zg-320T/zg-336 had highest similarity falsenii DSM 44353T (97.51 %), coyleae 44184T (98.68 zg-913T/zg-915 afermentans subsp. lipophilum CIP 103500T (98.79 %)....
Two Gram-stain-positive, irregular rod-shaped (0.2-0.5×1.3-2.5 um) strains, HY056T and HY057, were isolated from the faeces of Equus kiang (the largest wild asses) collected at different regions Qinghai-Tibetan Plateau PR China. Phylogenetic analyses indicated that strains HY057 belong to genus Nocardioides by sharing a similarity ranging 96.3 97.0 % in 16S rRNA gene sequence forming distinct cluster with daphniae JCM 16608T, houyundeii 78T, solisilvae Ka25T gilvus XZ17T. The digital DNA-DNA...
A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which incidentally captured in plateau pika burrow on Qinghai-Tibet Plateau, PR China. Analysis 16S rRNA gene sequence showed that strain CF-49T closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), fluvialis NCFB 2497T (96.0 %) and lutrae 39187T (95.9 %), whereas similarity another isolate (CF-210) 99.9 %. Strains CF-210 grew optimally at...
Four novel strains isolated from the cloacal contents of snow finches ( Montifringilla taczanowskii ) were characterized as aerobic, Gram-stain-negative, slightly motile, and rod-shaped. Analysis 16S rRNA gene sequence revealed that strain CF-458 T had highest similarities 96.9 96.4 % with Limnobaculum parvum HYN0051 Pragia fontium DSM 5563 , while CF-1111 shared 96.1 Pantoea rodasii LMG 26273 Pectobacterium punjabense SS95 . Phylogenomic analysis showed four isolates separated into group Ⅰ...
Four unknown strains, characterized as Gram-stain-negative, strictly anaerobic, non-motile and rod-shaped, were isolated from fresh faeces of healthy humans in PR China. Pairwise sequence comparisons the 16S rRNA genes showed that these isolates separated into two clusters. Cluster I (strains HF-5141 T HF-106) was most closely related to Bacteroides xylanisolvens XB1A (98.0–98.3 % similarity) ovatus ATCC 8483 (97.3–97.5 %), whereas cluster II HF-5287 HF-5300) exhibited a similarity range...
Four unknown strains belonging to the genus Arthrobacter were isolated from plateau wildlife on Qinghai-Tibet Plateau of PR China. Phylogenetic analysis based 16S rRNA gene sequences showed that four isolates separated into two clusters. Cluster I (strains 785T and 208) had greatest sequence similarity citreus (98.6 98.7 %, respectively), luteolus (98.0 98.1%, gandavensis (97.9 98.0 respectively) koreensis (97.6 97.7 respectively). Likewise, cluster II J391T J915) highest ruber 98.3 agilis...
Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in Qinghai-Tibet Plateau PR China. The cells aerobic, oxidase-negative catalase-positive. Colonies yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood. grew optimally °C, pH 7.5 1 (w/v) NaCl BHI supplemented Phylogenetic analysis 16S...
Four obligatory anaerobic, Gram-stain-positive, non-motile and rod-shaped organisms (HF-1365T, HF-1362, HF-1101T HF-4214) were isolated from faecal samples of healthy Chinese subjects. Results 16S rRNA gene sequence analyses showed that these isolates belong to the genera Enorma (strains HF-1365T HF-1362) Eggerthella HF-4214), closest massiliensis (both 98.6 %) sinensis (98.0 97.8 %), respectively. The whole genome sequences strains 2.3 4.2 Mb in size with 61.7 66.2 mol% DNA G+C content,...
The species in the genus Erwinia are Gram-stain-negative, facultatively anaerobic, motile, and rod-shaped. Most phytopathogens. Also, persicina was involved several human infections. Based on reverse microbial etiology principles, it is worth analyzing pathogenicity of this genus. In study, we isolated sequenced two Erwinia. Phylogenetic, phenotypic, biochemical, chemotaxonomic analyses were performed to identify its taxonomy position. virulence tests plant leaves pear fruits used...
A polyphasic taxonomic characterization of two novel strain pairs (designated zg-579T/zg-578 and zg-536T/zg-ZUI104) isolated from the faeces Marmota himalayana was conducted based on phylogenetic analysis nearly full-length 16S rRNA gene genome, digital DNA-DNA hybridization, ortho-average nucleotide identity (Ortho-ANI), phenotypic chemotaxonomic traits. Comparative sequences showed that zg-579T most closely related to Nocardioides dokdonensis FR1436T (97.57 %) deserti SC8A-24T (97.36 %),...
Land expansion and population growth are the most important factors for urban development in rapid urbanization stage. Intensive land use is a key goal of sustainable development. Therefore, to promote intensive use, local governments should take scientific evaluation relationship between as basis make policies cautiously. Thus, it vital adopt methods that sensitive use. However, this topic has received insufficient attention literature. Addressing gap, study (1) proposes modified method...
Objective To evaluate the effect of up-converting phosphor technology(UPT) in detection plague antigen-antibody by receiver operating characteristic curve (ROC) method,and to provide a scientific basis for field application UPT rapid technology prevention and control.Methods Two hundred twenty four serum samples were collected from Marmots ground squirrels foci,Yersinia pestis antibody was detected UPT,ELISA,Colloidal-gold Strips IHA,respectively; 108 organs bone marrow collected,and...
Objective To type Yersinia pestis isolated from Gansu Province, and to study the trend of diffreent strains in different administrative regions years. Methods Totally 193 were enrolled this study, including 9 Ganning Dauricus type, 18 Aerjin 45 Qilian 121 Qingzang type. These genotyped by clustered regularly interspaced short palindromic repeats (CRISPR), genotypes named according international standard. Genotyping CRISPR years Province was explored. Results Two clusters (Ca7...
Objective: To study the clustered regularly interspaced short palindromic repeats (CRISPR) loci polymorphism of Yersinia pestis and its area distribution in Gansu province. Methods: A total 203 strains isolated from 1962 to 2014 were selected for culture extraction DNA. Three pairs CRISPR primers used amplify strain DNA by PCR, PCR products sequenced. The groups genotypes determined according spacer arrangement strain. Cluster analysis was done using software BioNumerics 5.10. Results: 16...