A5022623172- Gut microbiota and health
- Pharmacogenetics and Drug Metabolism
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- Cancer-related molecular mechanisms research
- Escherichia coli research studies
- Microbial Community Ecology and Physiology
- Pharmaceutical studies and practices
- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Circular RNAs in diseases
- Molecular Biology Techniques and Applications
- Digestive system and related health
- SAS software applications and methods
- Spacecraft Dynamics and Control
- Statistical Methods in Clinical Trials
- Bioinformatics and Genomic Networks
- Bacterial Identification and Susceptibility Testing
- Respiratory and Cough-Related Research
- Astronomy and Astrophysical Research
- Spacecraft Design and Technology
- Bacterial biofilms and quorum sensing
- HIV/AIDS drug development and treatment
- Digital Imaging for Blood Diseases
Næstved Sygehus
2011-2024
University College Absalon
2020-2024
University of Copenhagen
2017-2023
Danish Technological Institute
2020
VIA University College
2020
Roskilde University
2013-2020
Region Zealand
2016
Statens Serum Institut
2012-2015
Novozymes (Denmark)
2003
APEC causes a range of infections in poultry, collectively called colibacillosis, and is the leading cause mortality associated with major economic significance poultry industry. A growing number studies have suggested as an external reservoir human ExPEC, including UPEC, which reservoir. ExPEC belonging to ST95 considered one most important pathogens both humans. This study first in-depth whole-genome-based comparison E. coli investigates core genomes well accessory avian ExPEC. We...
Wnt signaling is often constitutively active in colorectal cancer cells. The expression of the intestinal specific transcription factor CDX2 found to be transiently decreased invasive cells at tumor/stroma interface. A recent ChIP-Seq study has indicated that several signaling-related genes are regulated by CDX2. aim was investigate role level on APC , AXIN2 and GSK3β migrating colon front. CDX2-bound promoter enhancer regions from were analyzed for gene regulatory activity pattern front...
Abstract Aim Diarrhoea is very common in children attending day care centres. The aim of this study was to examine certain predisposing risk factors for an association with diarrhoea, including foreign travel, treatment antibiotics, having household pets, infant colic, bottle feeding, using a pacifier and low birthweight. Methods A dynamic one‐year follow‐up cohort comprising 179 from 36 centres conducted September 2009 July 2013 Copenhagen, Denmark. Questionnaires were sent the children's...
A proof-of-concept for the fabrication of a self-polymerizing system sampling gut microbiome in upper gastrointestinal (GI) tract is presented. An orally ingestible microdevice loaded with reaction mixture to entrap microbiota and biomarkers. This polymerization activated aqueous environment, like fluids intestinal lumen, causes site-specific microsampling tract. The sampled protein biomarkers can be isolated analyzed via high-throughput multiomic analyses. study utilizes hollow (Su-8, ca....
A plasmid‐encoded ClpK protein was recently identified as a predictor of heat‐resistant phenotype in the opportunistic pathogen K lebsiella pneumoniae . This study undertaken to evaluate presence clpK gene extended‐spectrum β‐lactamase ( ESBL )‐producing and assess probable co‐transfer multi‐resistance with heat resistance phenotype. D anish collection 80 ‐producing bloodstream infection isolates screened for by colony hybridization. Nineteen (24%) were positive ; some them representing...
Abstract MicroRNAs are small regulatory RNAs that deregulated in a wide variety of human cancers, including different types B-cell lymphoma. Nevertheless, the feasibility circulating microRNA for early diagnosis lymphoma has not been established. To address possibility detecting specific microRNAs years before is diagnosed, we studied plasma expression first pre-treatment samples from patients with diffuse large and subsequently repository blood donors who later developed lymphomas. In...
Background: The cytochrome P450 drug metabolizing enzymes CYP2D6 and CYP2C19 are the major targets for pharmacogenomics (PGx) testing determining response. Clinical dosing guidelines specific drug-gene interactions (DGI) publicly available through PharmGKB. aim of this register study was to map use drugs in Denmark having actionable (AG) i.e., recommendations different from standard or DGI terms consumption. Methods: Danish Register Medicinal Product Statistics source retrieve consumption...
Infection in the oviduct (salpingitis) is most common bacterial infection egg laying hens and mainly caused by Escherichia coli. The disease responsible for decreased animal welfare, considerable economic loss as well a risk of horizontal vertical transmission pathogenic E. outcome salpingitis may be either acute or chronic. It has not yet been clarified whether pathological manifestation result characteristics coli associated with host factors such immunity.From core- accessory genome...
The cytochrome P450 (CYP) enzyme family catalyses the metabolism of approximately 80% all medications.1, 2 Many genes encoding CYP enzymes exhibit high levels polymorphism that affect their expression and activity. most frequent clinically important variations exist within CYP2D6, CYP2C19 CYP2C9.1 extensively studied drug-metabolizing enzyme, has over 100 allelic variants is responsible for metabolizing about 25% marketed 3 CYP2C9 are collectively 20% medications. It estimated 74%–97%...
Background World Health Organization guidelines now recommend routine use of viral load testing, where available, for patients receiving antiretroviral treatment (ART). However, its has not been routinely implemented in many resource‐limited settings due to cost, availability and accessibility. Viral testing is complex, making application challenging. We describe the issues encountered by Médecins Sans Frontières (MSF) when using a large HIV programme sub‐Saharan Africa. Methods Between...
Polypharmacy is most prevalent among the elderly population and in particular nursing home residents. The frequency of use drugs with pharmacogenomics (PGx)-based dosing guidelines for CYP2D6, CYP2C9, CYP2C19 SLCO1B1 were measured residents Capital Region Denmark as well drug-drug interactions. aim was to evaluate potential applying PGx-test a supportive tool medication reviews. Drug during 2017-2018 Copenhagen, PGx-based available through PharmGKB website, measured. Drug-drug interactions...
To examine whether inactivation of the dlt operon and increased charge density wall enhances secretion heterologous proteins in industrial strains Bacillus licheniformis.The dltA gene B. licheniformis was cloned, sequenced mutated by inserting a chloramphenicol acetyl transferase (cat) cassette. The mutation facilitated growth late exponential phase, endogenous autolysis decreased resistance to cationic peptide, polylysine. It observed that production cyclodextrin glycosyltransferase...
Abstract Escherichia coli causing infection outside the gastrointestinal system are referred to as extra-intestinal pathogenic E. coli. Avian is a subgroup of and infections due avian have major impact on poultry production economy welfare worldwide. An almost defining characteristic carriage plasmids, which may encode virulence factors antibiotic resistance determinates. For same reason, plasmids been intensively studied. However, genes encoded by chromosome also be important for disease...
The mammalian Caudal-related homeobox transcription factor 2 (CDX2) plays a key role in the regulatory network and is essential regulating expression of several (HOX) genes during embryonic development, particularly gut. Genome-wide CDX2 chromatin immunoprecipitation analysis data from Caco2 cells also suggests for regulation HOXB4 gene intestinal epithelium. Thus, aim this study was to investigate whether regulated by We demonstrated binding four different sites an enhancer region located...
Cytochromes P450 (CYP450) drug metabolizing enzymes are the key in catalysing oxidative biotransformation of 70%–80% all drugs clinical use to either inactive metabolites or active substances.1, 2 Polymorphism genes encoding CYP450 family has attracted considerable attention as major targets for pharmacogenomics (PGx) testing since they highly polymorphic and thereby determining response adverse reactions (ADRs).3-5 CYP2C9 CYP2C19 both members CYP2C superfamily located on chromosome 10. Both...
Genotyping of the CYP2D6 gene is most commonly applied pharmacogenetic test globally. Significant economic interests have led to development a plurality assays, available for almost any genotyping platform or DNA detection chemistry. Of all genetic variants, copy number variations are particular difficult detect by polymerase chain reaction. Here, we present two simple novel approaches identification samples carrying either deletions duplications gene; relative quantification using...