A5088726057- MicroRNA in disease regulation
- Circular RNAs in diseases
- Ovarian cancer diagnosis and treatment
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Health Systems, Economic Evaluations, Quality of Life
- Cancer Cells and Metastasis
- Cancer Mechanisms and Therapy
- PARP inhibition in cancer therapy
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- Genomics and Rare Diseases
- Extracellular vesicles in disease
- Statistical Methods in Clinical Trials
- Tissue Engineering and Regenerative Medicine
- Cancer-related molecular mechanisms research
- Genetic factors in colorectal cancer
- Schizophrenia research and treatment
- Kruppel-like factors research
- Inflammatory mediators and NSAID effects
- RNA modifications and cancer
- Cardiac electrophysiology and arrhythmias
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
- Wnt/β-catenin signaling in development and cancer
JSS Academy of Higher Education and Research
2025
Mayo Clinic in Arizona
2018-2023
Mayo Clinic
2021-2023
WinnMed
2023
Case Western Reserve University
2015-2019
Case Comprehensive Cancer Center
2016-2018
Cornell University
2015-2017
Mysore Medical College
2016
// Anil Belur Nagaraj 1 , Peronne Joseph Olga Kovalenko Sareena Singh 2 Amy Armstrong Raymond Redline 3 Kimberly Resnick Kristine Zanotti Steven Waggoner and Analisa DiFeo Case Comprehensive Cancer Center, Western Reserve University, Cleveland, OH, USA Department of Gynecology, Division Gynecological Oncology, University Hospital Medical Pathology, Correspondence to: DiFeo, email: Keywords : patient derived xenograft, platinum resistance, tumor spheres, cancer initiating cells,...
Computation-based drug-repurposing/repositioning approaches can greatly speed up the traditional drug discovery process. To date, systematic and comprehensive computation-based to identify validate drug-repositioning candidates for epithelial ovarian cancer (EOC) have not been undertaken. Here, we present a novel strategy that combines computational system (DrugPredict) with biological testing in cell lines order rapidly EOC. DrugPredict exploited unique repositioning opportunities rendered...
Effective targeting of cancer stem cells (CSCs) requires neutralization self-renewal and chemoresistance, but these phenotypes are often regulated by distinct molecular mechanisms. Here we report the ability to target both via CD55, an intrinsic cell surface complement inhibitor, which was identified in a comparative analysis between CSCs non-CSCs endometrioid models. In this context, CD55 functions complement-independent manner required lipid raft localization for CSC maintenance cisplatin...
// Chantelle D. Hudson 1 , Alyssa Savadelis Anil Belur Nagaraj 2 Peronne Joseph Stefanie Avril 3 Analisa DiFeo 2, * Norbert 1, Department of Radiology, Case Center for Imaging Research, Western Reserve University, Cleveland, OH 44106, USA Comprehensive Cancer Center, Pathology, Shared senior authors Correspondence to: Avril, email: Norbert.Avril@Case.edu Keywords: ovarian cancer, cisplatin resistance, glutamine metabolism, glutaminase inhibitors Received: February 13, 2016 Accepted: April...
// Andrew Wiechert 1, 2, * , Caner Saygin Praveena S. Thiagarajan 1 Vinay Rao James Hale Nikhil Gupta 3 Masahiro Hitomi 3, 4 Anil Belur Nagaraj 5 Analisa DiFeo Justin D. Lathia 4, Ofer Reizes Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA 2 Division Gynecological Oncology, Women's Health Clinic College Medicine Case Western Reserve University, 44195 at Comprehensive Cancer Center, 44106, These authors contributed equally...
Abstract Wnt signaling is a major driver of stemness and chemoresistance in ovarian cancer, yet the genetic drivers that stimulate its expression remain largely unknown. Unlike other cancers, mutations pathway are not reported high-grade serous cancer (HGSOC). Hence, key challenge must be addressed to develop effective targeted therapies identify nonmutational activation. Using an miRNA sensor-based approach, we have identified miR-181a as novel Wnt/β-catenin signaling. miR-181ahigh primary...
Multiple sclerosis (MS) is a chronic autoimmune illness characterized by demyelination, neurodegeneration, and inflammation in the central nervous system. The AXL gene, which codes for receptor tyrosine kinase, has emerged as promising therapeutic target due to its involvement neuroinflammation oligodendrocyte dysfunction. In current study, we employed silico techniques design Antisense Oligonucleotides (ASOs) that selectively gene transcripts modulate expression mitigate MS pathology. Three...
Inherited pathogenic variants in PALB2 are associated with increased risk of breast and pancreatic cancer. However, the functional clinical relevance many missense uncertain significance (VUS) identified through genetic testing is unclear. The ability patient-derived germline VUS to disrupt function was assessed identify potential relevance.The influence 84 on evaluated using a cellular homology directed DNA repair (HDR) assay impacting activity were further characterized secondary...
Characterizing the complex interplay of cellular processes in cancer would enable discovery key mechanisms underlying its development and progression. Published approaches to decipher driver do not explicitly model tissue-specific changes pathway networks regulatory disruptions related genomic aberrations cancers. We therefore developed InFlo, a novel systems biology approach for characterizing biological using unique multidimensional framework integrating transcriptomic, and/or epigenomic...
Abstract Pathogenic protein-truncating variants of RAD51C, which plays an integral role in promoting DNA damage repair, increase the risk breast and ovarian cancer. A large number RAD51C missense uncertain significance (VUS) have been identified, but effects majority these on function cancer predisposition not established. Here, analysis 173 by a homology-directed repair (HDR) assay reconstituted RAD51C−/− cells identified 30 nonfunctional (deleterious) variants, including 18 hotspot within...
Abstract miRNAs are important regulators of diverse cellular processes including proliferation, apoptosis, and differentiation. In the context bone marrow derived stromal cell adipose differentiation, established both differentiation or stemness depending on their target. Furthermore, miRNA dysregulation can play a key role in various disease states. Here we show that miR-181a is regulated circadian manner induced during immortalized (iBMSC) as well primary patient (PASC) adipogenesis....
ABSTRACT Effective targeting of cancer stem cells (CSCs) requires neutralization self-renewal and chemoresistance, however these phenotypes are often regulated by distinct molecular mechanisms. Here we report the ability to target both via CD55, an intrinsic cell surface complement inhibitor, which was identified in a comparative analysis between CSCs non-CSCs endometrioid models. In this context, CD55 functions complement-independent manner required lipid raft localization for CSC...
Tumor-initiating cells (TICs) contribute to drug resistance and tumor recurrence in cancers, thus experimental approaches dissect the complexity of TICs are required design successful TIC therapeutic strategies. Here, we show that miRNA-3′ UTR sensor vectors can be used as a pathway-based method identify, enrich, analyze from primary solid patient samples. We have found an miR-181ahigh subpopulation sorted ovarian exhibited properties vivo, were enriched response continuous cisplatin...
Abstract Purpose: Acquired resistance to cisplatin is a major barrier success in treatment of various cancers, and understanding mitotic mechanisms unique cisplatin-resistant cancer cells can provide the basis for developing novel targeted therapies aimed at eradicating these cells. Experimental Design: Using models derived from primary patient epithelial ovarian (EOC) cells, we have explored status exit Results: We uncovered an unexpected role long-term inducing vulnerability characterized...
Epithelial ovarian cancer (EOC) is one of the deadliest gynecological cancers currently diagnosed in women with approximately 20,000 new cases and 15,000 deaths per year. Ovarian can be stratified into distinct subtypes which high-grade serous (HGSOC) comprises 90% all has highest mortality rate. Reducing high rate associated HGSOC two primary barriers: 1) most HGSOCs are at a post-metastatic stage 2) up to 70% patients recur within 5 years. Both these limitations due fact that drivers tumor...
<div>Abstract<p>Pathogenic protein-truncating variants of RAD51C, which plays an integral role in promoting DNA damage repair, increase the risk breast and ovarian cancer. A large number RAD51C missense uncertain significance (VUS) have been identified, but effects majority these on function cancer predisposition not established. Here, analysis 173 by a homology-directed repair (HDR) assay reconstituted RAD51C-/- cells identified 30 non-functional (deleterious) variants,...
<p>Supplementary Materials, References, Tables and Figures</p>
<p>Supplementary Materials, References, Tables and Figures</p>
Abstract Epithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy and fifth leading cause of deaths in women. The common form EOC which makes up greater than 75% cases high-grade serous (HGSOC). Platinum-based drugs are used as first-line chemotherapeutics for all women diagnosed with HGSOC. Unfortunately, ~20% do not respond to this treatment 80% those patients recur within 5 years at point particularly difficult eradicate. Emerging evidence supports concept that platinum...
Background: Second-generation antipsychotics (SGAs) are better for psychotic disorders, and they can induce weight gain other serious metabolic adverse effects which lead to non-adherence medical comorbidities. Iloperidone asenapine, the newer SGAs with favorable short-term side effect profile. Aims Objective: The aim of this study was compare propensity associated new drugs-asenapine iloperidone. Materials Methods: Randomized prospective conducted from December 2014 August 2015 in...