A5068365860- Adipose Tissue and Metabolism
- Muscle Physiology and Disorders
- Cellular Mechanics and Interactions
- Diet and metabolism studies
- Circadian rhythm and melatonin
- Adipokines, Inflammation, and Metabolic Diseases
- Sirtuins and Resveratrol in Medicine
- Dietary Effects on Health
- Exercise and Physiological Responses
- Graphene and Nanomaterials Applications
- Mitochondrial Function and Pathology
- Nutrition and Health in Aging
- Digestive system and related health
- Peptidase Inhibition and Analysis
- Mesenchymal stem cell research
- Neurobiology and Insect Physiology Research
- Radiopharmaceutical Chemistry and Applications
- Muscle metabolism and nutrition
- Metabolism, Diabetes, and Cancer
- Autophagy in Disease and Therapy
- Ubiquitin and proteasome pathways
Novo Nordisk Foundation
2018-2024
University of Copenhagen
2018-2024
Abstract Reactive oxygen species (ROS) act as intracellular compartmentalized second messengers, mediating metabolic stress-adaptation. In skeletal muscle fibers, ROS have been suggested to stimulate glucose transporter 4 (GLUT4)-dependent transport during artificially evoked contraction ex vivo, but whether myocellular production is stimulated by in vivo exercise control metabolism unclear. Here, we combined humans and mice with fluorescent dyes, genetically-encoded biosensors, NADPH...
Abstract Mitochondrial dysfunction and low nicotinamide adenine dinucleotide (NAD + ) levels are hallmarks of skeletal muscle ageing sarcopenia 1–3 , but it is unclear whether these defects result from local changes or can be mediated by systemic dietary cues. Here we report a functional link between circulating the natural alkaloid trigonelline, which structurally related to nicotinic acid 4 NAD health in multiple species. In humans, serum trigonelline reduced with correlate positively...
Diabetes represents a major public health concern with considerable impact on human life and healthcare expenditures. It is now well established that diabetes characterized by severe skeletal muscle pathology limits functional capacity quality of life. Increasing evidence indicates also one the most prevalent disorders impaired regeneration, yet underlying mechanisms therapeutic treatments remain poorly established. In this review, we describe cellular molecular alterations currently known...
NAD+ is a co-factor and substrate for enzymes maintaining energy homeostasis. Nicotinamide phosphoribosyltransferase (NAMPT) controls synthesis, in skeletal muscle, essential muscle integrity. However, the underlying molecular mechanisms by which synthesis affects health remain poorly understood. Thus, objective of current study was to delineate role NAMPT-mediated biosynthesis development function. To determine Nampt function, we generated muscle-specific KO (SMNKO) mice. We performed...
Molecular clocks in the periphery coordinate tissue-specific daily biorhythms by integrating input from hypothalamic master clock and intracellular metabolic signals. One such key signal is cellular concentration of NAD + , which oscillates along with its biosynthetic enzyme, nicotinamide phosphoribosyltransferase (NAMPT). levels feed back into to influence rhythmicity biological functions, yet whether this fine-tuning occurs ubiquitously across cell types a core feature unknown. Here, we...
Except for latitudes close to the equator, seasonal variation in light hours can change dramatically between summer and winter. Yet investigations into interplay energy metabolism circadian rhythms typically use a 12 h light:12 dark photoperiod corresponding duration at equator. We hypothesized that altering affects both rhythmicity of peripheral tissue clocks homeostasis. Mice were housed photoperiods representing either summer, winter, or equinox. winter exhibited an increase amplitude...
Dysregulation of skeletal muscle metabolism influences whole-body insulin sensitivity and glucose homeostasis. We hypothesized that type 2 diabetes–associated alterations in the plasma metabolome directly contribute to immunometabolism subsequent development resistance. To this end, we analyzed metabolite profile identified glutamine as a key amino acid correlates inversely with BMI resistance index (HOMA-IR) men normal tolerance or diabetes. Using an vitro model human myotubes vivo...
BACKGROUNDDuring aging, there is a functional decline in the pool of muscle stem cells (MuSCs) that influences and regenerative capacity skeletal muscle. Preclinical evidence has suggested nicotinamide riboside (NR) pterostilbene (PT) can improve regeneration, e.g., by increasing MuSC function. The objective this study was to investigate if supplementation with NR PT (NRPT) promotes regeneration after injury elderly individuals improved recruitment MuSCs.METHODSThirty-two (55-80 years age)...
Circadian rhythms can be perturbed by shift work, travel across time zones, many occupational tasks, or genetic mutations. Perturbed circadian are associated with the increasing problem of obesity, metabolic dysfunction, and insulin resistance. We hypothesized that sensitivity in skeletal muscle follows a pattern this is important for overall function. This hypothesis was verified using mice as model system. observed rhythmicity whole body tolerance, well signaling pathways regulating...
Metabolic dysfunction and Type 2 diabetes are associated with perturbed circadian rhythms. However, exercise appears to ameliorate disturbances, as it can phase-shift or reset the internal clock system. Evidence is emerging that at a distinct time of day correct misalignments influence energy metabolism. This suggests timing training be important for prevention management metabolic dysfunction. In this study, obese, high-fat diet-fed mice were subjected voluntary wheel running (VWR) two...
Skeletal muscle is an endocrine organ secreting exercise-induced factors (exerkines), which play a pivotal role in interorgan cross talk. Using mass spectrometry (MS)-based proteomics, we characterized the secretome and identified thymosin β4 (TMSB4X) as most upregulated secreted protein media of contracting C2C12 myotubes. TMSB4X was also acutely increased plasma exercising humans irrespective insulin resistance condition or exercise mode. Treatment mice with did not ameliorate metabolic...
ABSTRACT Reactive oxygen species (ROS) act as intracellular compartmentalized second messengers mediating metabolic stress-adaptation. In skeletal muscle fibers, ROS have been suggested to stimulate glucose transporter 4 (GLUT4)-dependent transport during artificially evoked contraction ex vivo but whether myocellular production is stimulated by in exercise control metabolism unclear. Here, we combined humans and mice with fluorescent dyes, genetically-encoded biosensors, NADPH oxidase 2...
Abstract Background Maintenance and regeneration of functional skeletal muscle are dependent on a sufficient pool stem cells (MuSCs). During ageing there is decline in this cellular which influences the regenerative capacity muscle. Preclinical evidence have suggested that Nicotinamide Riboside (NR) Pterostilbene (PT) can improve e.g. by increasing MuSC function. The objective present study was to investigate if NRPT supplementation promotes after injury elderly humans improved recruitment...
Dysregulation of skeletal muscle metabolism influences whole body insulin sensitivity and glucose homeostasis. We hypothesized that type 2 diabetes-associated alterations in the plasma metabolome directly contribute to immunometabolism subsequent development resistance. To this end, we analyzed metabolite profile identified glutamine as a key amino acid correlated inversely with mass index (BMI) HOMA-IR men normal tolerance or diabetes. Using an <i>in vitro</i> model human...
Dysregulation of skeletal muscle metabolism influences whole body insulin sensitivity and glucose homeostasis. We hypothesized that type 2 diabetes-associated alterations in the plasma metabolome directly contribute to immunometabolism subsequent development resistance. To this end, we analyzed metabolite profile identified glutamine as a key amino acid correlated inversely with mass index (BMI) HOMA-IR men normal tolerance or diabetes. Using an <i>in vitro</i> model human...