A5000668254- Marine Ecology and Invasive Species
- Marine Invertebrate Physiology and Ecology
- Retinoids in leukemia and cellular processes
- Developmental Biology and Gene Regulation
- interferon and immune responses
- Estrogen and related hormone effects
- Planarian Biology and Electrostimulation
- Coral and Marine Ecosystems Studies
- Marine Biology and Environmental Chemistry
- RNA and protein synthesis mechanisms
- Marine Bivalve and Aquaculture Studies
- Ichthyology and Marine Biology
- RNA Research and Splicing
- Cardiomyopathy and Myosin Studies
- Proteoglycans and glycosaminoglycans research
- Fish Ecology and Management Studies
- Bioinformatics and Genomic Networks
- Congenital heart defects research
- Antioxidant Activity and Oxidative Stress
- Plant-derived Lignans Synthesis and Bioactivity
- Chemokine receptors and signaling
- Fish Biology and Ecology Studies
- Renal and related cancers
- Phytochemical compounds biological activities
- Adipose Tissue and Metabolism
Centre National de la Recherche Scientifique
2014-2025
Inserm
2019-2025
Université Côte d'Azur
2019-2025
Institut de Recherche sur le Cancer et le Vieillissement de Nice
2019-2025
Sorbonne Université
2014-2024
Laboratoire de Biologie du Développement de Villefranche-sur-Mer
2013-2024
Institut de la Mer de Villefranche
2015-2024
Jackson Laboratory
2023
Murdoch Children's Research Institute
2023
ORCID
2023
Elevations in cytosolic free calcium concentration ([Ca(2+)](cyt)) constitute a fundamental signal transduction mechanism eukaryotic cells, but the molecular identity of Ca(2+) channels initiating this plants is still under debate. Here, we show by pharmacology and loss-of-function mutants that tobacco Arabidopsis, glutamate receptor-like (GLRs) facilitate influx across plasma membrane, modulate apical [Ca(2+)](cyt) gradient, consequently affect pollen tube growth morphogenesis....
The question of whether the ancestral bilaterian had a central nervous system (CNS) or diffuse ectodermal has been hotly debated. Considerable evidence supports theory that CNS evolved just once. However, an alternative view proposes chordate from nerve net hemichordate-like deuterostome, implying independent evolution in chordates and protostomes. To specify morphological divisions along anterior/posterior axis, this ancestor used gene networks homologous to those patterning three...
ABSTRACT A model organism in developmental biology is defined by its experimental amenability and resources created for the system scientific community. For most powerful invertebrate models, combination of both has already yielded a thorough understanding processes. However, number systems still limited, their phylogenetic distribution heavily biased. Members one largest animal lineages, Spiralia, example, have long been neglected. In order to remedy this shortcoming, we produced detailed...
Chordates are divided into three subphyla: Vertebrata, Tunicata, and Cephalochordata. Phylogenetically, the Cephalochordata, more commonly known as lancelets or amphioxus, constitute sister group of Vertebrata Tunicata. Lancelets small, benthic, marine filter feeders, their roughly dozen described species genera:
Optimized laboratory conditions for research models are crucial the success of scientific projects. This includes controlling entire life cycle, having access to all developmental stages and maintaining stable physiological conditions. Reducing cycle a model can also enhance biological material speed up genetic tool development. Thus, we optimized rearing sea anemone Nematostella vectensis, cnidarian study embryonic post-metamorphic processes, such as regeneration. We adopted semi-automated...
Mixed Lineage Leukemia 5 (MLL5) plays a key role in hematopoiesis, spermatogenesis and cell cycle progression. Chromatin binding is ensured by its plant homeodomain (PHD) through direct interaction with the N-terminus of histone H3 (H3). In addition, MLL5 contains Su(var)3-9, Enhancer zeste, Trithorax (SET) domain, protein module that usually displays lysine methyltransferase activity. We report here crystal structure unliganded SET domain human at 2.1 Å resolution. Although it shows most...
Although chordates descend from a segmented ancestor, the evolution of head segmentation has been very controversial for over 150 years. Chordates generally possess pharynx, but even though anatomical evidence and gene expression analyses suggest homologies between pharyngeal apparatus invertebrate chordates, such as cephalochordate amphioxus, vertebrates, these remain contested. We, therefore, decided to study chordate by examining molecular mechanisms underlying morphogenesis in an animal...
Abstract For over a century, researchers have been trying to understand the relationship between embryogenesis and regeneration. A long-standing hypothesis is that biological processes implicated in embryonic development are re-deployed during In past decade, we begun relationships of genes their organization into gene regulatory networks (GRN) driving regeneration diverse taxa. Here, compare GRNs same species investigate how re-uses genetic interactions originally set aside for development....
During embryogenesis, tight regulation of retinoic acid (RA) availability is fundamental for normal development. In parallel to RA synthesis, a negative feedback loop controlled by catabolizing enzymes the cytochrome P450 subfamily 26 (CYP26) crucial. vertebrates, functions three CYP26 (CYP26A1, CYP26B1, and CYP26C1) have been well characterized. By contrast, outside little known about complements their biological roles. an effort characterize evolutionary diversification catabolism, we...
An ontology is a computable representation of the different parts an organism and its developmental stages as well relationships between them. The model organisms therefore fundamental tool for multitude bioinformatics comparative analyses. cephalochordate amphioxus marine animal representing earliest diverging evolutionary lineage chordates. Furthermore, morphology, anatomy genome can be considered prototypes chordate phylum. For these reasons, very important biology studies aimed at...
During development, morphogens, such as retinoic acid (RA), act mediators of intercellular communication systems to control patterning and cell fate specification processes. In vertebrates, the tightly regulated production degradation RA creates an anterior-posterior (A-P) morphogen gradient that is required for regional embryo. catabolism in particular, mediated by members cytochrome P450 subfamily 26 (CYP26), has been highlighted a key regulatory component formation this gradient....
We report here a robust and efficient protocol for the expression of fluorescent proteins after mRNA injection into unfertilized oocytes cephalochordate amphioxus, Branchiostoma lanceolatum. use constructs membrane nuclear targeted mCherry eGFP that have been modified to accommodate amphioxus codon usage Kozak consensus sequences. describe type needles be used, immobilization oocytes, overall set-up. This technique generates fluorescently labeled embryos, in which dynamics cell behaviors...
Abstract Chordates are divided into three subphyla: Vertebrata, Tunicata and Cephalochordata. Phylogenetically, the Cephalochordata, more commonly known as lancelets or amphioxus, constitute sister group of Vertebrata Tunicata. Lancelets small, benthic, marine filter feeders, their roughly dozen described species genera: Branchiostoma , Epigonichthys Asymmetron . Due to phylogenetic position stereotypical chordate morphology genome architecture, key models for understanding evolutionary...
We performed studies in 8-, 16-, 24-, 30 and 35-day-old Wistar rats at 8.00 h (AM) 20.00 (PM) to investigate the relationship between diurnal variations of basal plasma corticosterone (compound B, CB) its responses ACTH ether stress during postnatal period. Basal CB levels increased PM from 8 35 days age an AM-PM difference was observed 16 days. Although responsiveness detected only 24 days, induced increasingly higher response than AM A stress-induced starting presented age-dependent...
Abstract Cardiac function requires appropriate proteins in each chamber. Atria slow myosin to act as reservoirs, while ventricles demand fast for swift pumping. Myosins are thus under chamber-biased cis -regulation, with gene expression imbalances leading congenital heart dysfunction. To identify regulatory inputs cardiac expression, we computationally and molecularly dissected the quail Slow Myosin Heavy Chain III ( SMyHC ) promoter that drives preferential atria. We show states...
Abstract Optimized laboratory conditions for research models are crucial the success of scientific projects. This includes control entire life cycle, access to all developmental stages and maintaining stable physiological conditions. Reducing cycle a model can also enhance biological material speed up genetic tool development. Thus, we optimized rearing sea anemone Nematostella vectensis , cnidarian study embryonic post-metamorphic processes, such as regeneration. We adopted semi-automated...
We report here a robust and efficient protocol for the expression of fluorescent proteins after mRNA injection into unfertilized oocytes cephalochordate amphioxus, Branchiostoma lanceolatum. use constructs membrane nuclear targeted mCherry eGFP that have been modified to accommodate amphioxus codon usage Kozak consensus sequences. describe type needles be used, immobilization oocytes, overall set-up. This technique generates fluorescently labeled embryos, in which dynamics cell behaviors...