A5009959505- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
- RNA Research and Splicing
- Meat and Animal Product Quality
- Sulfur Compounds in Biology
- Animal Genetics and Reproduction
- Gut microbiota and health
- Cytokine Signaling Pathways and Interactions
- Cardiomyopathy and Myosin Studies
- Effects of Environmental Stressors on Livestock
- Clostridium difficile and Clostridium perfringens research
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Folate and B Vitamins Research
- Musculoskeletal pain and rehabilitation
- Exercise and Physiological Responses
- Muscle metabolism and nutrition
- Animal Nutrition and Physiology
- Acupuncture Treatment Research Studies
- RNA modifications and cancer
- Adipokines, Inflammation, and Metabolic Diseases
- Fibroblast Growth Factor Research
Shanxi Agricultural University
2019-2025
Henan University
2021-2024
Chinese Academy of Agricultural Sciences
2015-2023
Institute of Animal Sciences
2015-2023
China Agricultural University
2015-2019
Kaiser Permanente Vacaville Medical Center
2019
University of Minnesota
2015
Kaiser Permanente San Francisco Medical Center
2010-2013
Tufts University
2003-2007
Boston Medical Center
2000-2005
Abstract Myostatin (MSTN) is a dominant inhibitor of skeletal muscle development and growth. Mutations in MSTN gene can lead to hypertrophy or double-muscled (DM) phenotype cattle, sheep, dog human. However, there has not been reported significant phenotypes pigs association with mutations. Pigs are an important source meat production, as well serve preferred animal model for the studies human disease. To study impacts mutations on growth pigs, we generated -mutant Meishan no marker via zinc...
Circular RNA (circRNA) has a significant impact on the maturation of skeletal muscle, although their precise functions within this framework remain largely uncharted. This study presents an investigation regulatory effect circAGGF1 myogenesis in myoblasts, including potential molecular mechanisms involved. It is revealed that facilitates differentiation myoblasts into other states while simultaneously enhancing manifestation type I muscle fibers. In vivo investigations with mice promotion...
// Chunbo Cai 1, 2 , Lili Qian Shengwang Jiang 1 Youde Sun 4 Qingqing Wang Dezun Ma Gaojun Xiao Biao Li Shanshan Xie Ting Gao 3 Yaoxing Chen Jie Liu 5 Xiaorong An Wentao Cui Kui Institute of Animal Sciences, Chinese Academy Agricultural Beijing, 100193, P. R. China State Key Laboratory Agro Biotechnology, University, College Medicine, Qingdao, 266100, Department Bioengineering and Chemical Engineering, Qingdao University Science Technology, 266042, Correspondence to: Cui, email:...
Circular RNA (circRNA), a novel class of non-coding RNA, has closed-loop structure with important functions in skeletal muscle growth. The purpose this study was to investigate the role differentially expressed circRNAs (DEcircRNAs), as well DEcircRNA-miRNA-mRNA regulatory network, at different stages porcine development. Here, we present panoramic view circRNA expression from Large White and Mashen pigs 1, 90, 180 days age.We identified total 5819 circRNAs. DEcircRNA analysis showed 327...
Gastrointestinal (GI) microbiota play an important role in promoting growth piglets. However, studies on composition at various stages are lacking. We measured body weights of Jinfen White and Mashen piglets every 7 days collected their fecal samples by rectal swabbing nine time points during suckling (1-28 days) nursery (35-70 to gain insight into variability piglet growth. The were characterized via 16S rRNA gene sequencing analyze the effects microbial diversity development preliminarily....
Muscle development is closely related to meat quality and production. CircRNAs, with a closed-ring structure, have been identified as key regulator of muscle development. However, the roles mechanisms circRNAs in myogenesis are largely unknown. Hence, order unravel functions myogenesis, present study explored circRNA profiling skeletal between Mashen Large White pigs. The results showed that total 362 circRNAs, which included circIGF1R, were differentially expressed two pig breeds....
The two breeds, Mashen (MS, a northern China breed) and Large White (LW, western lean pigs, show important phenotypic differences in growth, adaptability, intramuscular fat (IMF) content energy metabolism since early developmental stage. main aim of this study was the evaluation longissimus thoracis muscle transcriptome profile both genetic types to identify genes, pathways responsible for their differentiated phenotype. Longissimus MS LW pigs were sampled at 0 day (early stage), 90 days...
This study investigated the effect of Flammulina velutipes polysaccharides (FVPs) on myofibrillar protein (MP) oxidation and physicochemical properties catfish surimi during 75 days frozen storage at −18°C. FVP was added to 1%, 1.5%, 2%, respectively; degree MP were investigated, microstructure observed by scanning electron microscopy (SEM). The results showed that carbonyl content thiobarbituric acid reactive substances (TBARS) in groups lower than those CK group (the blank surimi). In...
Clitocybe squamulose, as a nutritious edible fungus, is widely distributed in Wutai Mountain (Shanxi, China), and rarely used for stewing chicken soup. Here, the effects of squamulose addition on Chinese three-yellow soup quality were analyzed. The results showed that significantly increased contents soluble solid matter, water-soluble protein, total sugar, 5′-GMP (guanosine-5′-monophosphate disodium salt), umami sweet free amino acids (P < 0.05), absolute value Zeta potential, reduced...
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Abstract Background Skeletal muscle development and fat deposition have important effects on meat quality. The study of regulating skeletal is great significance in improving the quality carcass meat. In present study, whole transcriptome sequencing (including RNA-Seq miRNA-Seq) was performed longissimus dorsi (LDM) Jinfen White pigs at 1, 90, 180 days age. Results results showed that a total 245 differentially expressed miRNAs were screened any two comparisons, which may be involved...
Inflammation accompanies hepatic dysfunction resulting from tissue oxidative damage. Naringenin (Nar), a natural flavanone, has known antioxidant and anti-inflammatory activities, but its mechanism of action in the regulation liver requires further investigation. In this study, role naringenin lipopolysaccharide (LPS)-induced stress inflammation was explored, as well by transcriptome sequencing. The results indicated that compared with LPS group, Nar treatment caused significant increase...
Cold stress influences intestinal processes, causing physiological and immunological responses in animals. Intestinal microbiota participates maintaining the stability of environment. However, phenotypic characteristics effects porcine changes under cold conditions remain poorly understood. Here, fecal tolerant breed (Mashen) sensitive (Duroc-Landrace-Yorkshire) was transferred to germ-free mice, respectively. After a exposure (4°C) for 21 days, function microbe mice were explored. The...
Myostatin (MSTN) is a key muscle factor that negatively regulates skeletal growth and development. Our laboratory recently produced genetically engineered Meishan pigs containing ZFN-edited MSTN loss-of-function mutation (MSTN-/-, MKO) led to the hypertrophy of muscles. In this study, we performed transcriptome sequencing miRNA in samples from MKO wildtype (MWT) investigate effect MSTN-/- on expression mRNA miRNA. results indicated that, compared MWT pigs, there were 200 genes 4 miRNAs being...
The growth and development of skeletal muscle is regulated by many factors, recent studies have shown that circular RNAs (circRNAs) can participate in this process. model porcine injury was constructed to search for circRNAs regulate the pigs. Using whole-transcriptome sequencing bioinformatics analysis, a novel circRNA (circCSDE1) screened out, which highly expressed muscle. Functional C2C12 cells demonstrated circCSDE1 could promote proliferation inhibit myoblast differentiation, while...
Hydrogen sulfide (H2 S) has been widely recognized as one of gasotransmitters. Endogenous H2 S plays a crucial role in the progression cancer. However, effect endogenous on development nasopharyngeal carcinoma (NPC) is still unknown. In this study, aminooxyacetic acid (AOAA, an inhibitor cystathionine-β-synthase), dl-propargylglycine (PAG, cystathionine-γ-lyase), and l-aspartic (l-Asp, 3-mercaptopyruvate sulfurtransferase) were adopted to detect NPC growth. The results indicated that combine...
Myostatin (MSTN) is a negative regulator of skeletal muscle growth and development. The in MSTN−/– mice significantly hypertrophied, with fiber type II increasing while I decreasing. However, it still not clear how the types change pigs, mechanism for MSTN regulates types, especially large animals like pigs. This study conducted comprehensive analysis composition fibers pigs produced our laboratory. It was observed that, compared wild-type (WT) both total mass IIb increased (P<0.01), IIa...
As a diverse and abundant class of endogenous RNAs, circular RNAs (circRNAs) participate in various biological processes including cell proliferation apoptosis. Nevertheless, few researchers have investigated the role circRNAs muscle development cultivated pigs.In this study, we used RNA-seq to construct circRNA expression profiles skeletal Jinfen White pigs at age 1, 90, 180 days. Among 16,990 identified circRNAs, 584 were differentially expressed. Moreover, enrichment analysis DE host...