Pre-eclampsia is a common complication during pregnancy; however, the underlying mechanisms of crosstalk between low-density lipoprotein receptor-related protein 6 (LRP6) and autophagy in trophoblast cells are still not fully explored. Messenger RNA (mRNA) levels LRP6, beclin 1, Unc-51-like activating kinase 1 (ULK1), p62, vimentin, matrix metallopeptidase-9 (MMP-9), β-catenin, c-Myc, Rab7, as well ratio LC3-II/LC3-I, were analysed by quantitative real-time polymerase chain reaction or...

To clarify the roles of claudins in endometrial tumorigenesis, we determined levels protein and messengerRNA (mRNA) expression claudin-3 claudin-4 human tissue (proliferative phase [PE, n = 25]; secretory [SE, simple hyperplasia [SH, 20]; complex [CH, 12]; atypical [AH, 15]; endometrioid adenocarcinoma [EEC, 30]) using immunohistochemistry, western blotting, real-time polymerase chain reaction, respectively. Morphologic changes tight junctions (TJs) were also observed normal, hyperplastic,...

Recent studies demonstrate that there are sex differences in the expression of angiotensin receptor type 2 (AT2-R) kidney and AT2-R plays an enhanced role regulating blood pressure (BP) females. Also, brain activation has been reported to negatively modulate BP sympathetic outflow. The present study investigated whether central blockade endogenous augments deoxycorticosterone acetate (DOCA)/salt-induced hypertension both male female rats.All rats were subcutaneously infused with DOCA...

To investigate the expression of advanced glycation end products (AGEs) and receptor for AGE (RAGEs) in maternal blood, umbilical blood placental tissues women with severe preeclampsia (sPE) as well any association inflammatory processes.The expressions AGEs, RAGE, tumor necrosis factor-alpha (TNF)-α vascular cell adhesion molecule-1 (VCAM)-1 were measured using immunohistochemistry. The levels TNF-α VCAM-1 extracts assessed enzyme-linked immunosorbent assays. Placental mRNA determined by...

The present study investigated whether central activation of angiotensin II type 2 receptor (AT2-R) attenuates deoxycorticosterone acetate (DOCA)/NaCl-induced hypertension in intact and ovariectomized (OVX) female rats sex hormone status has influence on the effects AT2-R activation. DOCA/NaCl elicited a greater increase blood pressure OVX females than that females. Central infusion compound 21, specific agonist, abolished pressor effect females, whereas same treatment produced an inhibitory...

Widespread in citrus fruits, naringin, a natural 2,3-dihydroflavonoid, is of particular interest to scientists and has broad range beneficial bioactivities health. Orally administered naringin remains the gut tract for relatively long time because its low bioavailability. Under metabolism mediated by human microbiota, could be an active precursor derived metabolites play important physiological roles. However, are hard accurately quantify due severe endogenic interference. In this study,...

Accumulating evidence indicates that maternal high-fat diet (HFD) is associated with metabolic syndrome and cardiovascular disease in adult offspring. The present study tested the hypothesis HFD modulates brain renin-angiotensin system (RAS), oxidative stress, proinflammatory cytokines alter angiotensin II (ANG II) TNF-α actions sensitize ANG II-elicited hypertensive response All offspring were cross fostered by dams on same or opposite to yield following four groups: from normal-fat control...

Abstract Aim Pre‐eclampsia (PE) is a pregnancy complication characterized by new onset maternal hypertension and proteinuria. Its underlying mechanisms are unclear. This study investigated the relationship between progesterone endoplasmic reticulum stress (ERS) associated apoptosis induced interleukin (IL)‐1β via glucose regulated protein 78 (GRP78)/protein kinase RNA‐like (PERK)/C/EBP‐homologous (CHOP) pathway in BeWo cells. Methods Venous blood placental tissues were collected from PE...

Abstract Background Clear cell renal carcinoma (ccRCC) is one of the most prevalent malignant diseases in urinary system with more than 140,000 related deaths annually. Ubiquitination–deubiquitination homeostasis an important factor ccRCC progression; ubiquitin‐specific peptidase 53 (USP53) belongs to family deubiquitinating enzymes, but its functions are rarely reported. Methods Databases obtained from GEO and TCGA were analyzed reveal role USP53 ccRCC. CCK‐8/BrdU EDU assays used detect...

Quercetin 7-rhamnoside (Q7R) is one of the main flavonoid components Hypericum japonicum. However, whether Q7R active ingredients responsible for hepatopreventive effects japonicum has not yet been ascertained. Thus, aim present study was to elucidate attenuates apoptosis induced by glycochenodeoxycholic acid (GCDC) in vitro, and mechanisms involved. L-02 human normal liver cells were pre-incubated with 0, 50, 100 200 µM 30 min then exposed GCDC indicated periods time....

Abstract Background This study aimed to investigate the effect of typhaneoside on ventricular remodeling and regulation PI3K/Akt/mTOR autophagy transduction pathway in rats with heart failure after myocardial infarction. Methods The effects general condition were observed vivo using a rat model infarction had been established. expression serum N‑terminal pro-brain natriuretic peptide (NT-proBNP), matrix lysin 2 (ST2), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α),...

Naoshuantong capsule (NSTC) is an oral Chinese medicine formula composed of Typhae Pollen, Radix Paeoniae Rubra, Curcumae Radix, Gastrodiae Rhizoma and Rhapontici. It has been widely used at the acute recovery stage ischemic stroke since 2001. Comparing with its wide clinical application, there are only few studies emphasize on investigating pharmacological effects.To more generally elucidate underlying mechanisms in this study, we identified active ingredients NSTC by a network pharmacology...

Heart failure (HF) is characterized by cardiac dysfunction along with autonomic unbalance that associated increased renin‐angiotensin system (RAS) activity and elevated levels of proinflammatory cytokines (PICs). Renal denervation (RD) has been shown to improve function in HF, but the protective mechanisms remain unclear. The present study tested hypothesis RD ameliorates isoproterenol‐ (ISO‐) induced HF through regulation brain RAS PICs. Chronic ISO infusion resulted remarked decrease blood...

Radiotherapy plays an essential role in the treatment of non-small-cell lung cancer (NSCLC). However, cells' resistance to ionizing radiation (IR) is primary reason for radiotherapy failure leading tumor relapse and metastasis. DNA double-strand breaks (DSB) repair after IR mechanism resistance. In this study, we investigated effects autophagy-inducing agent, Rapamycin (RAPA), combined with histone deacetylase inhibitor (HDACi), Suberoylanilide Hydroxamic Acid (SAHA), on radiosensitivity...

Objective: To determine whether maternal plasma human placental lactogen (hPL) mRNA levels can predict abnormally invasive placenta. Study design: Sixty-eight singleton pregnant women with prior Cesarean deliveries were classified into three groups: 35 normal placentation (control group); 21 placenta previa alone (placenta 12 and accreta group). Maternal hPL concentrations measured by real-time reverse-transcription polymerase chain reaction Result: The multiple of the median (median, range)...

Maternal high-fat diet (HFD) is associated with metabolic syndrome and cardiovascular diseases in adult offspring. Our previous study demonstrated that maternal HFD enhances pressor responses to ANG II or a proinflammatory cytokine (PIC), which increased expression of brain renin-angiotensin system (RAS) components PICs The present further investigated whether inhibition angiotensin-converting enzyme (ACE) tumor necrosis factor-α (TNF-α) blocks sensitization hypertension offspring dams. All...

It is still urgent and challenge to develop a simple risk assessment scale for venous thromboembolism (VTE) in puerperium Chinese women.The study, retrospective case-control was conducted 12 hospitals different cities China. A total of 1152 pregnant women were selected, including 384 cases with VTE 768 without VTE. logistic regression method determine the factors VTE.Age, BMI before delivery, gestational diabetes mellitus, family history (thrombosis, diabetes, cardiovascular disease),...

Objective. To investigate the inhibitory effect of ethyl acetate extracts Impatiens balsamina L. on prostate cancer cells. Methods. was extracted to get water, ethanol, oil ether, acetate, and butanol extracts. CCK-8 assay used detect effect. Apoptosis rates cell cycle distribution were detected by flow cytometry. Transwell performed test ability migration. The expressions Bcl-2, Bax, cleaved-caspase-3, p-ERK, ERK, p-AKT, AKT, cyclin D1, E, MMP2 Western blot. Results. Ethyl had strongest...