Following prolonged exposure to hypoxic conditions, for example, due ascent high altitude, aging or stroke, cognitive deficits can develop. The exact nature and genesis of hypoxia-induced remain unresolved. Curcumin has been reported stimulate neurogenesis reduce neuronal degeneration. This study aimed investigate the effect curcumin on in hypoxic-brain injury mice its potential mechanism. Eight-week-old male C57BL/6J were normobaric-hypoxia (13%O2) 14 days establish models. Morris water...

Colorectal cancer is one of the most common malignancies both in men and women. Owing to metastasis resistance, prognosis colorectal cancerCRC patients remains extremely poor with chemotherapy. A disintegrin metalloproteinase 17 (ADAM17) induces activation Notch pathway contributes chemoresistance. This study aimed discover a novel ADAM17 inhibitor investigate chemosensitization effect.Pharmacophore model, western blot enzymatic assay were used ZLDI-8. Cell proliferation was determined by...

Amplifications at 6p22.3 are prevalent in advanced stage bladder cancer (TCC). Previous studies have identified SOX4, CDKAL, and E2F3 as targets of this amplification therefore potential oncogenes, but the more telomeric DEK gene too has been reported overexpressed amplified. We investigated whether intermediate region harboring oncogene candidate ID4 is also part amplicon.Expression E2F3, DEK, was by real-time RT-PCR 28 TCC compared to 6 normal tissues 15 cell lines cultured urothelial...

To investigate the spatiotemporal expression of cannabinoid receptor type 2 (CB2R) in hippocampus pilocarpine-treated rats experiencing a status epilepticus (SE). A total 112 male Sprague-Dawley at three weeks age, weighing 43 g–68.5 g, were used for this study. Rats randomly divided into control group (n = 16) and an epilepsy SE group. The was further 6 sub-groups (2 h, 1, 3, 7, 14, 21 days after (SE)). model induced with lithium chloride pilocarpine by intraperitoneal injection....

Background Hashimoto’s thyroiditis (HT) is an autoimmune disease that features activation of thyroid antigen-specific helper T cells. HT patients have increased Th1 and Th17 cell subsets. Glycolysis supports chronic cells, but how this contributes to remains unknown. Methods The metabolism CD4+ cells from 30 healthy controls was evaluated by determining the extracellular acidification rate (ECAR) oxygen consumption (OCR). Mice in a subacute (SAT) model were treated with 2DG, metformin, or...

Abstract Objective To evaluate the clinical impact of ketogenic diet ( KD ) on children with pharmacoresistant epileptic encephalopathy. Methods In all, 52 encephalopathy that diagnosed in our hospital from July 2012 to June 2015 were selected, including West syndrome 38 cases, Lennox–Gastaut Syndrome 7 Doose 1 case, and Dravet 6 effect, compliance, adverse reactions, electroencephalogram EEG ), cognitive function analyzed. Modified Johns Hopkins protocol was used initiate , Engel scale...

Abstract Aim To investigate whether diabetes and fasting blood glucose (FBG) levels affect the efficacy of remote ischaemic conditioning (RIC) using database included in Remote Ischaemic Conditioning for Acute Moderate Stroke (RICAMIS) trial. Methods A total 1707 patients were enrolled this post hoc study, including 535 with 1172 without diabetes. Each group was further divided into RIC control subgroups. The primary outcome excellent functional outcome, defined as a modified Rankin Scale...

Abstract Background and purpose The neutrophil‐to‐lymphocyte ratio (NLR) has been demonstrated as a prognostic inflammatory biomarker in ischemic stroke. study aimed to investigate the association of NLR its dynamic change with long‐term outcome mortality acute stroke (AIS) patients who received intravenous thrombolysis (IVT). Methods From prospective cohort, AIS receiving IVT (alteplase, 0.9 mg/kg) complete data were retrospectively screened. Based on 3‐month modified Rankin scale score...

The mechanism of apoptosis via the p53‑dependent pathway remains to be fully understood. In present study, a novel p53 target gene, Unc5D, was identified and its possible function in human neuroblastoma cells investigated. apoptotic effects Unc5D SK‑N‑BE (p53‑/‑) SH‑SY5Y (p53+/+) were measured by an 3‑(4,5‑dimethylthiazol‑2‑yl)2,5‑diphenyltetrazolium bromide solution assay. Reverse transcription‑polymerase chain reaction (RT‑PCR) also performed detect endogenous expression Unc5D. H1299...

Neuronal loss and gliosis are the major pathological changes after status epilepticus (SE). The authors' previous study revealed time‑dependent of cannabinoid receptor type 2 (CB2R) in hippocampal neurons developing rats SE, which were accompanied by a decrease number neurons. Meanwhile, growing evidence indicates that CB2R stimulation exerts anti‑convulsant properties seizure models. However, activation neuronal repair response to damage SE is still unclear. In this experiment,...

Background: Idiopathic pulmonary fibrosis (IPF) is a life-threatening disease, the progression of which current drug therapy cannot reverse. This study analyzed research hotspots and future trends in IPF through bibliometric methods, with aim providing reference for new therapeutic strategies. Methods: Publications on obtained from Web Science Core Collection database, The Literature Metrology Online Analysis Platform, CiteSpace were used to analyze publication characteristics. VOSviewer was...

Objective Studies have long shown that uncontrolled inflammatory responses in the brain play a key role epilepsy pathogenesis. Microglias an important epileptic-induced neuroinflammation, but their after epileptic seizures is still poorly understood. Alleviating and its comorbidities has become area of interest for pediatricians. Methods A pilocarpine-induced rat model was established. The rats were randomly divided into four groups: control group, TLR4 inhibitor group (epilepsy+TAK-242),...

Abstract Objective This study investigated the clinical, imaging, and electroencephalogram (EEG) characteristics of methylmalonic acidemia (MMA) with nervous system damage as primary manifestation. Methods From January 2017 to November 2022, patients injury main clinical manifestation, diagnosed by metabolic genetic testing, were enrolled analyzed. Their data Results A total 18 enrolled, including 15 males 3 females. The symptoms convulsions, poor feeding, growth retardation, disorder...

Ras homologue gene family member A (RhoA) is involved in tumor mobility, invasion, and metastasis. We detected RhoA expression vulvar squamous cell carcinoma (VSCC) tissue, measured the VSCC phenotype, of relevant molecules after small interfering RNA (siRNA) transfection SW962 cells. has a higher level than normal vulva skin tissue was positively associated with International Federation Gynecology Obstetrics (FIGO) stage differentiation; besides, patients lymph node metastasis had positive...

The KCNC2 gene encodes Kv3.2, which is a member of the voltage-gated potassium channel subfamily. It crucial for generation fast-spiking properties in cortical GABAergic interneurons. Recently, variations were found to be associated with epileptic encephalopathy unrelated individuals. Here, we report Chinese patient developmental and (DEE) motor development delay. Whole-exome sequencing (WES) revealed novel heterozygous variant NM_139137.4:c.1163T>C (p.Phe388Ser), subsequent Sanger showed...

Medical imaging plays an increasingly important role in the diagnosis and treatment of disease. Currently, medical equipment mainly has two-dimensional (2D) systems. Although this conventional largely satisfies clinical requirements, it cannot depict pathologic changes 3 dimensions. The development three-dimensional (3D) technology encouraged advances imaging. Three-dimensional offers doctors much more information on a pathology than 2D imaging, thus significantly improving diagnostic...

Abstract Background and purpose The relationship between ionized calcium prognosis of ischemic stroke is controversial. We aim to determine the admission levels with acute (AIS) after intravenous thrombolysis (IVT). Methods Consecutive anterior circulation AIS patients treated recombinant tissue plasminogen activator (rt‐PA) were retrospectively enrolled. According quartiles, divided into four groups clinical data analyzed groups. Ionized was entered logistic regression analysis in two...