A5001961835- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Use and Resistance
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Escherichia coli research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial infections and disease research
- Iron Metabolism and Disorders
- Plant Virus Research Studies
- Magnesium in Health and Disease
- Pneumonia and Respiratory Infections
- Computational Drug Discovery Methods
- Tuberculosis Research and Epidemiology
- Clinical Laboratory Practices and Quality Control
- Microbial Natural Products and Biosynthesis
- Trace Elements in Health
Yonsei University
2015-2023
Glasgow Royal Infirmary
2022
University of Glasgow
2022
Severance Hospital
2018
Infections caused by carbapenem-resistant Acinetobacter baumannii (CRAB) present significant healthcare challenges due to limited treatment options. Bacteriophage (phage) therapy offers potential as an alternative treatment. However, the high host specificity of phages poses for their therapeutic application. To broaden phage spectrum, laboratory-based training using Appelmans protocol was employed in this study. As a result, successfully expanded range cocktail targeting CRAB. Further...
Carbapenemase-producing organisms (CPO) are rapidly disseminating worldwide, and their presence in tertiary care hospitals poses a significant threat to the management of nosocomial infections. There is need control CPO, especially intensive unit (ICU) patients, because these resistant most β-lactam antibiotics easily transmitted. At present, identification CPO time-consuming; hence, this study focused on use Xpert CARBA-R assay (Cepheid, USA) determine intestinal colonization rates patients...
Acinetobacter spp. have emerged as significant pathogens causing nosocomial infections. Treatment of these has become a major challenge to clinicians worldwide, due their increasing tendency antibiotic resistance. To address this, much revenue and technology are currently being dedicated toward developing novel drugs combinations combat antimicrobial this issue, we constructed panel strains expressing different resistance determinants such narrow spectrum β-lactamases, extended-spectrum...
This study investigates GT-1 (also known as LCB10-0200), a novel-siderophore cephalosporin, inhibited multidrug-resistant (MDR) Gram-negative pathogen, via Trojan horse strategy exploiting iron-uptake systems. We investigated activity and the role of siderophore uptake systems, combination non-β-lactam β-lactamase inhibitor (BLI) diazabicyclooctane, GT-055, referred to LCB18-055) against molecularly characterised resistant Escherichia coli, Klebsiella pneumoniae Acinetobacter spp. isolates....
Klebsiella pneumoniae is responsible for numerous infections caused in hospitals, leading to mortality and morbidity. It has been evolving as a multi-drug resistant pathogen, acquiring multiple resistances such horizontal gene transfer, transposon-mediated insertions or change outer membrane permeability. Therefore, constant efforts are being carried out control the using various antibiotic therapies. Considering severity of acquired resistance, we developed panel strains K. expressing...
Klebsiella pneumoniae is a major bacterial pathogen that causes pneumonia and urinary tract infections in human. K. are treated with carbapenem, but carbapenem-resistant has been spreading worldwide. We able to identify antimicrobial-resistant genes among mutated of the antibiotic-resistant clinical isolates. However, they usually harbor many genes, including those cause weak or neutral functional effects. Therefore, we need prioritize more likely candidates for follow-up analysis. For this...
Abstract Background Small colony and capnophilic variant cases have been separately reported, but there has no reports of their simultaneous presence in one isolate. We report a case Escherichia coli with coexpressed small phenotypes causing misidentification automated biochemical kits non-reactions antimicrobial susceptibility test cards. Case presentation An 86-year-old woman developed urinary tract infection from strain SCV co-existence. This did not grow without the CO 2 , therefore...
The existing modified carbapenem inactivation methods (mCIMs) recommended by the CLSI for detecting carbapenemase production have not been applicable Acinetobacter baumannii. We evaluated influence of matrices used in mCIMs and CIMTris on stability disks producers suggested optimal mCIM conditions carbapenemase-producing A. baumannii.Seventy-three baumannii isolates characterized antimicrobial susceptibility encoding genes were tested using CIMTris. (Tryptic soy broth [TSB] Tris-HCl) these...
<h2>Summary</h2><h3>Background & aims</h3> Interpretation of blood micronutrient levels requires age-appropriate reference intervals. This study developed age-dependent centiles for healthy children (HC) and explored their utility in sick children. <h3>Methods</h3> 244 samples were collected from normal HC who underwent tests acute illness. Age-dependent, centile charts fitted zinc, copper, magnesium selenium plasma erythrocytes (RBC), vitamins B1, B2 B6 RBC. For 34 with Crohn's disease...
The siderophore-antibiotic conjugate LCB10-0200 (a.k.a. GT-1) has been developed to combat multidrug-resistant Gram-negative bacteria. In this study, the in vitro activity of and LCB10-0200/avibactam (AVI) investigated against carbapenem-resistant Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa. Minimal inhibitory concentrations (MICs) LCB10-0200, LCB10-0200/AVI, aztreonam, aztreonam/AVI, ceftazidime, ceftazidime/AVI, meropenem were measured using...
Abstract Background A complex cascade of genes, enzymes, and transcription factors regulates AmpC β-lactamase overexpression. We investigated the network overexpression in Klebsiella aerogenes identified role AmpG resistance to β-lactam agents, including cephalosporins carbapenems. Methods transposon mutant library was created for carbapenem-resistant K. YMC2008-M09-943034 (KE-Y1) screen candidates with increased susceptibility carbapenems, which susceptible derivatives KE-Y3 KE-Y6. All...
Recently, a blaNDM-9 and mcr-1 co-harboring E. coli ST 617 isolate was identified from an asymptomatic carrier in Korea. An 81-year-old female admitted to university hospital for aortic cardiac valve repair surgery. Following surgery, she the intensive care unit (ICU) three days, carbapenem-resistant YMC/2017/02/MS631 isolated surveillance culture (rectal swab). Antimicrobial susceptibility testing (AST) colistin not performed at that time. Upon retrospective study, further AST revealed...
Quality control (QC) processes are being performed in the majority of clinical microbiology laboratories to ensure performance microbial identification and antimicrobial susceptibility testing by using ATCC strains. To obtain these strains, some inconveniences encountered concerning purchase cost strains shipping time required. This study was focused on constructing a database reference for QC domestic bacterial concentrating primarily testing. Three (Escherichia coli, Pseudomonas...
ABSTRACT We identified the carbapenemase gene bla OXA-499 , a variant of OXA-143 from clinical isolate Acinetobacter pittii for first time. shared 93.1% amino acid identity with OXA-143, and was located on chromosome. By cloning OXA-499-encoding into pWH1266 vector transforming it susceptible spp., we were able to show that confers resistance carbapenems.
To detect the outer membrane protein (OMP), which plays a key role in carbapenem resistance, whole-genome and transcriptome analysis of clinical carbapenem-resistant Klebsiella pneumoniae was carried out. The index strain lacked both OmpK35 OmpK36, whereas other strains only OmpK35. After SDS-PAGE, putative OMP bands were excised identified as OmpA OmpK36. MALDI-TOF MS showed peaks at ∼36 ∼38 kDa that corresponded to respectively. In all except YMC2014/03/P345, present. K. ATCC 13883 isolate...
Evolution of multi-drug resistant bacteria has led to worldwide research better understand the various resistance mechanisms in these strains. Every year, novel information on carbapenem and its is being discovered. In this study, radiation-mediated mutagenesis was used transform a carbapenem-resistant Klebsiella pneumoniae strain carbapenem-susceptible bacterium. Through process, we proved three conditions loss OmpK35 OmpK36 genes acquisition blaCMY-10 worked together produce K. pneumoniae....