A5102968221- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Virology and Viral Diseases
- Dengue and Mosquito Control Research
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Viral Infections and Outbreaks Research
- Animal Disease Management and Epidemiology
- Vector-borne infectious diseases
- Effects and risks of endocrine disrupting chemicals
- Viral gastroenteritis research and epidemiology
- Bacterial Identification and Susceptibility Testing
- Phytase and its Applications
- Protein Interaction Studies and Fluorescence Analysis
- Proteins in Food Systems
- Nanoparticle-Based Drug Delivery
- Advanced Biosensing Techniques and Applications
- Neurological diseases and metabolism
- Hereditary Neurological Disorders
- Animal Virus Infections Studies
- Environmental Quality and Pollution
- Insect symbiosis and bacterial influences
- Bacillus and Francisella bacterial research
- Hepatitis Viruses Studies and Epidemiology
Hunan Agricultural University
2024
Guangzhou Center for Disease Control and Prevention
2010-2024
Centers for Disease Control and Prevention
1992-2015
University of Alabama at Birmingham
2012
Guangzhou Eighth People's Hospital
2010
Lanzhou Institute of Husbandry and Pharmaceutical Sciences
1998
This study aimed to analyze the profiles and evolution of Staphylococcus aureus in pediatric intensive care units (PICUs) 17 hospitals China from 2016 2022. Susceptibility testing was performed bacterial strains with a uniform monitoring protocol, which provided by US Clinical Laboratory Standards Institute (CLSI) used Antimicrobial Surveillance Network (CHINET). The results were interpreted accordance performance standards for antimicrobial susceptibility issued Institute. Twenty-six...
Serotyping is a useful tool to study the epidemiologic characteristics of rotaviruses in large populations and assess need for vaccine protect against all strains. By using an enzyme immunoassay with serotype-specific monoclonal antibodies four most common rotavirus serotypes, we analyzed 1,183 rotavirus-positive specimens from 16 stool collections eight countries on continents that were obtained 1978 1989. Of 926 strains (78%) could be serotyped, 48% serotype 1, 8% 2, 15% 3, 7% 4....
Abstract To investigate molecular epidemiology of dengue viruses (DENV) in southern China, a total 14 isolates were collected China during each epidemic year between 1978 and 2006 their full-length genome sequences obtained by using RT-PCR method. The E gene from additional 6 fever patients Guangzhou also Combined with DENVs published GenBank, phylogenetic analysis recombination performed. One hundred twenty-five 60 complete the GenBank involved. Phylogenetic showed that there was wide...
Hand, foot and mouth disease (HFMD) is usually caused by Enterovirus 71(EV71), Coxsackievirus A16 (CV-A16) in Guangzhou, the biggest city of South China. However, A6 (CV-A6) were observed increased dramatically from 2010–2012. In order to understand describe epidemiologic genetic characteristics CV-A6, specimens 5482 suspected HFMD cases collected examined real-time fluorescence PCR. All samples positive for enteroviruses analyzed descriptive statistics. Phylogenetic analysis CV-A6 based on...
Abstract Background Dengue virus (DENV) infection is the most prevalent arthropod-borne viral in tropical and subtropical regions worldwide. Guangzhou has ideal environment for DENV transmission epidemics have been reported this region more than 30 years. Methods Information cases from 2001 to 2010 were collected analyzed. The strains cultured isolated patients’ sera. Viral RNA was extracted cell culture supernatants. cDNA synthesized by reverse transcription PCR. Phylogenetic trees of four...
Background Endemic dengue virus type 3 (DENV-3) infections have not been reported in Canton, China, since 1980. In March 2009, DENV-3 was isolated for the second time, occurring about 30 years after previous circulation. August, other cases emerged. One much larger outbreak occurred again 2010. To address origin and particularly to determine whether outbreaks were caused by same viral genotype, we investigated epidemiological molecular characteristics of introduction, spread genetic...
Cross-priming amplification (CPA) was evaluated for the early detection of norovirus (NV), rotavirus A (RV-A), enteric adenovirus (EAdV) and astrovirus (AstV). The analytical sensitivity CPA assay 10(3) copies ml(-1) NV, RV-A AstV 10(4) EAdV detection. For each four pathogens, positive rate by similar to real-time PCR methods higher than observed in an ELISA. coincidence rates RT-PCR RV-A, were 98, 99, 99 100%, respectively. All assays negative 89 healthy control samples. These results...
Abstract Background Dengue fever is a mosquito-borne infectious disease that has caused major health problems. Variations in dengue virus (DENV) genes are important features of epidemic outbreaks. However, the associations DENV with potential have not been extensively examined. Here, we assessed new genotype invasion DENV-1 isolated from Guangzhou China to evaluate Methodology/principal findings We used strains sera cases 2002 2016 for complete genome sequencing. A neighbor-joining...
Dengue virus (DENV) causes a wide range of diseases in humans, from the acute febrile illness dengue fever (DF) to life-threatening haemorrhagic fever/dengue shock syndrome. We developed four real-time quantitative PCR assays for each serotype DENV based on computational analysis. These had high sensitivity and specificity without cross-reactivity serotypes. To evaluate performance these detecting typing clinical samples, we analysed 64 serum samples Guangdong during 2006. The results showed...
Bluetongue virus (BTV), a member in the family Reoviridae, is re-emerging animal disease infecting cattle and sheep. With its recent outbreaks Europe, there pressing need for efficacious antivirals. We presented here identification characterization of novel virostatic molecule against BTV, an aminothiophenecarboxylic acid derivative named compound 003 (C003). The efficacy C003 could be improved via chemical modification, leading to de novo synthesized 052 (C052). 50% effective concentrations...
After an absence of 29 years, dengue virus type 3 (DENV-3) re-emerged in Guangzhou 2009 and again 2010. However, the geographical route by which entered city, how it has changed genetically, remain unclear. Therefore, we carried out a comprehensive investigation into molecular characteristics DENV-3 involved.The envelope (E) genes viruses isolated from patients during 2009-2010 epidemics were sequenced compared with previously published E gene sequences global representative strains...
In 2010, the first complete genome sequence of a dengue virus serotype 4 genotype II strain was reported in Guangzhou, China. Here, we report another isolated belonging to II. Our results will offer help control and precautions.
In 2009, dengue virus serotype 3 (DENV-3) was first detected in Guangzhou, China. this study, we identified another isolated strain belonging to genotype II. Phylogenetic analysis shows that the GZ/10476/2012 has a close relationship with DENV-3 II from Southeast Asian strains.
ABSTRACT This study aims to develop and validate a robust analytical method for the quantification of polybrominated diphenyl ethers (PBDEs) in human serum using gas chromatography–tandem mass spectrometry. We compared procedural blanks, recoveries, operational convenience liquid–liquid extraction supported liquid determination PBDEs. evaluated different solvents their effect on PBDE recoveries. Supported was selected validation due its convenience. The demonstrated satisfactory linearity,...