A5081194803- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Liver Disease Diagnosis and Treatment
- Drug-Induced Adverse Reactions
- Asthma and respiratory diseases
- Eicosanoids and Hypertension Pharmacology
- Neurological Disease Mechanisms and Treatments
- Traditional Chinese Medicine Analysis
- Liver physiology and pathology
- Flavonoids in Medical Research
- Urticaria and Related Conditions
- Plant-based Medicinal Research
- Inflammatory mediators and NSAID effects
- Alcohol Consumption and Health Effects
- Immune cells in cancer
- Drug-Induced Hepatotoxicity and Protection
- Ferroptosis and cancer prognosis
- Computational Drug Discovery Methods
- Helicobacter pylori-related gastroenterology studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Pharmacological Effects of Natural Compounds
- Cytokine Signaling Pathways and Interactions
- Cancer, Hypoxia, and Metabolism
- Berberine and alkaloids research
- Cancer therapeutics and mechanisms
Zhengzhou University
2016-2025
Vanderbilt University Medical Center
2017-2019
First Affiliated Hospital of Zhengzhou University
2018-2019
Beijing Proteome Research Center
2016
Machine Science
2013-2014
Chinese University of Hong Kong
2014
Canadian Standards Association
2013-2014
Institute of Electrical and Electronics Engineers
2013-2014
Inner Mongolia Medical University
2013
Capital Medical University
2013
The lack of information concerning individual variation in content and activity human liver microsomal protein is one the most important obstacles for designing personalized medicines. We demonstrated that mean value per gram (MPPGL) was 39.46 mg/g 128 livers up to 19-fold variations existed. Meanwhile, metabolic activities 10 cytochrome P450 (CYPs) were detected microsomes tissues, respectively, which showed huge (200-fold). Compared with microsomes, tissues much suitable express CYP...
Due to a lack of physiologic cytochrome P450 (P450) isoform content, activity is typically only determined at the microsomal level (per milligram protein) and not picomole isoform), which could result in misunderstanding variations between individuals further hinder development personalized medicine. We found that there were large protein mRNA levels, intrinsic activities 10 P450s 100 human liver samples, CYP2E1 CYP2C9 showed highest expression levels. gene polymorphisms had different...
// Jun Zhou 1, 2, * , Qiang Wen Sai-Fei Li 2 Yun-Fei Zhang 1 Na Gao Xin Tian Yan Fang Jie Ming-Zhu Cui Xiao-Pei He Lin-Jing Jia Han Jin Hai-Ling Qiao Institute of Clinical Pharmacology, Zhengzhou University, Zhengzhou, People’s Republic China Henan Provincal Hospital, These authors have contributed equally to this work Correspondence to: Qiao, email: qiaohl@zzu.edu.cn Keywords: hepatocellular carcinoma, cytochrome P450, human liver microsomes, drug metabolism, gene polymorphism...
Abstract The tumor microenvironment (TME) was usually studied in tissue and relation to only progression, with little involved occurrence, recurrence metastasis of tumor. Thus, a new concept “peritumor (PME)” proposed the proteomic characterization peritumor liver tissues human hepatocellular carcinoma (HCC). PME for occurrence (PME-O) progression (PME-P) were almost totally different at proteome composition function. Proteins rarely overlapped crossed. Immunity played central role PME-O,...
Background: Hepatocellular carcinoma (HCC) is a highly heterogeneous tumor, and distinguishing its subtypes holds significant value for diagnosis, treatment, the prognosis. Methods: Unsupervised clustering analysis was conducted to classify HCC subtypes. Subtype signature genes were identified using LASSO, SVM, logistic regression. Survival-related Cox regression, their expression function validated via qPCR gene interference. GO, KEGG, GSVA, GSEA used determine enriched signaling pathways....
The hepatic metabolism of patients with hepatocellular carcinoma and cirrhosis (HCCC) may differ from that (HCC) without cirrhosis, limiting the clinical individualized dosing regimens for these patients. Microsomal protein per gram liver (MPPGL) is an important scaling factor physiologically based pharmacokinetic models, but data in HCCC are limited. study aims to determine content MPPGL guide using vitro drug data. microsomal was determined samples (n=48) normal (n=68). activity 10...
Hepatocellular carcinoma (HCC) is one of the most common types primary liver cancer and third frequent cause death worldwide. Diethylnitrosamine (DEN) recognized risk factors for hepatocarcinogenesis likely due to CYP2E1-mediated metabolic activation. However, DEN activity in non-neoplastic tissue from HCC patients has not been determined; role CYP2E1 activity, particular constitutive inhibited with respect induced by yet clear. Herein, we determined found that was significantly elevated a...
// Jie Gao 1, * , Jun Zhou Xiao-Pei He 1 Yun-Fei Zhang Na Xin Tian Yan Fang Qiang Wen Lin-Jing Jia Han Jin Hai-Ling Qiao Institute of Clinical Pharmacology, Zhengzhou University, Zhengzhou, China These authors have contributed equally to this work Correspondence to: Qiao, e-mail: qiaohl@zzu.edu.cn Keywords: hepatocellular carcinoma, bottom up IVIVE approach, hepatic clearance, cytochrome P450s, microsomal protein per gram liver Received: February 14, 2016     Accepted:...
Human cytochrome P450 oxidoreductase (POR) provides electrons for all microsomal cytochromes (P450s) and plays an indispensable role in drug metabolism catalyzed by this family of enzymes. We evaluated 100 human liver samples found that POR protein content varied 12.8-fold, from 12.59 to 160.97 pmol/mg, with a median value 67.99 pmol/mg; mRNA expression 26.4-fold. activity was less variable 56.05 nmol/min per milligram. Cigarette smoking alcohol consumption clearly influenced activity. Liver...
Glioblastoma (GBM) is a devastating inflammation-related cancer for which novel therapeutic targets are urgently required. Previous studies of the authors indicate Cytochrome P450 2E1 (CYP2E1) as inflammatory target and develop specific inhibitor Q11. Here it demonstrated that CYP2E1 overexpression closely related to higher malignancy in GBM patients. activity positively correlated with tumor weight rats. Significantly expression accompanied by increased inflammation detected mouse model....
Abstract Lung cancer is the leading cause of death among all cancers. A persistent chronic inflammatory microenvironment highly correlated with lung cancer. However, there are no anti‐inflammatory agents effective against Cytochrome P450 2E1 (CYP2E1) plays an important role in response. Here, it found that CYP2E1 significantly higher peritumoral tissue non‐small cell (NSCLC) patients and tumor growth impeded Cyp2e1 −/− mice. The novel inhibitor Q11, 1‐(4‐methyl‐5‐thialzolyl) ethenone,...
Excessive production of interleukin (IL)-4, IL-13 and interferon (IFN)-gamma is thought to be important in the development allergic disease atopy. Several investigators have linked IL-4 IL-4R genes The aim this study further explore mechanism penicillins allergy evaluate possible role C-589T IL-4RalphaQ576R polymorphisms modulating responses penicillins.Radioallergosorbent test (RAST) was used detect eight kinds specific immunoglobulin E (IgE) serum. Serum levels IL-4, IFN-gamma were...
Human cytochrome b5 (Cyt b5) plays important roles in P450 (CYP)-mediated drug metabolism. However, the expression level of Cyt normal human liver remains largely unknown. The effect on overall CYP activity microsomes (HLM) has rarely been reported and relationship between polymorphic not systematically investigated. In this study, we found that median value protein was 270.01 pmol/mg from 123 HLM samples, 12- 19-fold individual variation observed mRNA levels, respectively. Gender smoking...
Baicalin, a flavonoid compound isolated from Scutellaria baicalensis , has been shown to possess antiinflammatory, antiviral, antitumour, and immune regulatory properties. The present study evaluated the potential herb-drug interaction between baicalin midazolam in rats. Coadministration of single dose (0.225, 0.45, 0.90 g/kg, i.v.) with (10 mg/kg, rats resulted dose-dependent decrease clearance (CL) 25% <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...
Baicalin purified from the root of Radix scutellariae is widely used in clinical practices. This study aimed to evaluate effect baicalin on pharmacokinetics nifedipine, a CYP3A probe substrate, rats vivo and vitro. In randomised, three-period crossover study, significant changes nifedipine (2 mg/kg) were observed after treatment with low (0.225 g/kg) or high (0.45 dose rats. low- high-dose groups baicalin-treated rats, Cmax total decreased by 40%±14% (P<0.01) 65%±14% (P<0.01), AUC0–∞ 41%±8%...
Baicalin has been used as mainly bioactive constituent of about 100 kinds traditional Chinese medicines in pharmacopoeia. The effect baicalin on cytochrome P450 should be paid more attention because was widely. aim this study to investigate whether could inhibit CYP1A2 pooled human liver microsomes (HLMs) and rats vivo the gene polymorphisms affect inter-individual variation IC50 28 livers. Phenacetin probe CYP1A2. Kinetic parameter each sample were measured common (−3860G>A −163C>A)...
// Jie Gao 1 , Gao-Ju Wang Zhao Na Jing Li Yun-Fei Zhang 1,2 Jun Zhou 1,3 Hong-Xin Qiang Wen Han Jin and Hai-Ling Qiao Institute of Clinical Pharmacology, Zhengzhou University, Zhengzhou, Henan, China 2 Affiliated Cancer Hospital 3 Provincial People's Correspondence to: Qiao, email: Keywords : cytochrome P450 2E1; hepatic fibrosis; causal role; prevention; diethylnitrosamine; Gerotarget Received July 06, 2017 Accepted November 14, Published December 05, Abstract Hepatofibrosis, which leads...