Hepatitis B virus (HBV) infection is a major public health problem. After HBV infection, viral antigens shift the immune balance in favor of escape. Sulforaphane (SFN) traditional Chinese medicine.It regulates multi-biological activities, including anti-inflammation, anticancer, and antiviral. However, few studies reported that SFN can inhibit before.

The objective of this study was to screen for antigens the hepatitis C virus (HCV) establish a new double antibody sandwich-lateral flow immunoassay (DAS-LFIA) method testing presence anti-HCV antibodies in human serum or plasma. A series different recombinant HCV proteins Escherichia coli cells were constructed, expressed, purified and DAS-LFIA strip developed. sensitivity specificity evaluated by detecting 23 HCV-positive sera, set quality control references detection that contain known...

Abstract Background and Aim The high mortality poor prognosis of hepatocellular carcinoma (HCC) have raised the public attention. Gene therapy is considered as a promising treatment option for cancer; thus, finding new therapeutic target HCC urgently needed. GATA4 tumor suppressor gene in multiple cancers, but its role unclear. In this study, we explored function HCC. Methods Reverse transcription–polymerase chain reaction quantitative polymerase were used to detect mRNA expression cells...

Despite the availability of an effective vaccine, hepatitis B virus (HBV) infection remains a major health problem. HBV e antigen (HBeAg)-negative strains have become prevalent. Previously, no animal model mimicked clinical course HBeAg-negative infection. To establish model, 3.2-kb full-length genome was cloned from sample and then circularized to form covalently closed circular (cccDNA). The resulting cccDNA introduced into liver C57BL/6J mice through hydrodynamic injection. Persistence...

Several studies have reported that hepatitis B virus (HBV) infection is mediated by macrophages and the B7x (B7-H4, VTCN-1) protein plays an important role in immune regulation HBV-associated hepatocellular carcinoma (HBV-HCC). However, relationship among HBV, macrophages, has not been studied. In this study, HBV-infected mouse model coculture of HBV cell lines were used to observe changes after infection. The expression markers (HBeAg, HBsAg), negative regulator immunity (B7x), T-helper 17...

A simple technique for the identification of common genotypes hepatitis B virus (HBV) remains to be identified. The present study was conducted establish such a methodology. Four plasmids A‑D and 123 clinical serum specimens HBV‑infected patients were genotyped. HBV would detected successfully when genotype reached viral load 1 x 103 copies/ml or BC mixed samples 5% level. lower limit detection DNA in determined 2.14x102 IU/ml. assay sensitivity specificity 100% consistency demonstrated...

Studies on molecular mechanisms of the persist infection hepatitis B virus have been hampered by a lack robust animal model. We successfully established simple, versatile, and reproducible HBV model in vitro vivo with circularized DNA. The cells mice were transfected or injected DNA pAAV/HBV1.2, respectively. At indicated time, cells, supernatants, serum samples, liver tissues collected for virological serological detection. Both vivo, pAAV/HBV1.2 could replicate transcribe efficiently, but...

High replication and mutation rates of hepatitis B virus (HBV) often lead to reduced or suppressed e antigen expression. The most common mutations are genomic variations in the basal core promoter (BCP) pre-core (PC) regions. However, effect BCP/PC on HBV phenotype vivo remains unclear. We compared analyzed reverse mouse models. In addition terminating expression HBeAg, also resulted a significant decrease HBsAg, DNA, cccDNA early stage, an obvious increase serum alanine aminotransferase...

Early detection of adefovir dipivoxil-resistant mutants during long-term treatment chronic hepatitis B virus (HBV) infection with this drug is great clinical importance.We developed an improved reverse dot hybridization test for simple and rapid the rtA181V/T rtN236T mutations associated dipivoxil resistance in patients.Probes were designed genotypes B, C, D characteristic; a total 70 samples analyzed assay.Its usefulness was validated by comparing sequencing data.Discordant results...

To establish a method for simultaneous detection of HBV resistant mutations associated with three kinds nucleoside analogues.According to 981 complete sequences in GenBank, two pairs conserved primers labeled digoxigenin were synthesized amplify the region reverse transcriptase. detect non-synonymous amino acid substitutions lamivudine, adefovir and entecavir, 26 specific oligonucleotide probes covering ten different codon positions, I169T, V173L/G, L180M, A181T/V, T184G, S202I/G, M204V/I,...

To generate a mouse model of chronic hepatitis B (CHB) infection by performing in vivo transduction virus (HBV) covalently closed circular (ccc)DNA.Nude mice were injected with HBV cccDNA at doses 1.5, 1.0 or 0.5 mug/ml. A control group was generated giving equal injection volumes physiological saline. The serum levels surface antigen (HBsAg) and e (HBeAg) on post-injection days 1 3, weeks 1-6, 8 10 assayed reflection immunoassay. At week 10, all animals sacrificed liver tissues collected....

GATA binding protein 4 (GATA4) has been reported as a potential target of gene therapy for hepatocellular carcinoma (HCC). It is well known that the main cause HCC chronic infection hepatitis B virus (HBV). However, whether effect GATA4 on HBV not yet reported.In this study, regulation was analyzed in vitro. In turn, also observed vitro, vivo, and clinical patients. Subsequently, we related to hepatocyte nuclear factor alpha (HNF4α) vitro.The results showed significantly promoted secretion...

To establish a detection method for HBV drug-resistant mutations related to lamivudine, adefovir and entecavir by optimization assessment of reverse hybridization system.26 degenerated probes covering 10 hotspots 3 drugs were synthesized immobilized on the same positively charged nylon membrane. PCR products labeled with digoxigenin hybridized corresponding probes. improve sensitivity specificity, 4 reaction steps optimized including number digoxigenin, energy intensity UV cross-linking,...

Comparative,medicine is a branch of laboratory animal seiences which also cuts the first edge morden medical sciences.This article mainly discussed origin comparative medicine,its contributions to life sciences.relationships between it and science,its importance for science researches.Most all.the necessarity feasibity teach medicine undergraduate students were highlighted Key words: Comparative medicine; Laboratory sciences; Undergraduate

Throughout the period of periimplantation, uterine endometrium is under constant remodeling and dynamic changes in a cell specific manner, which involves complex issues regards to apoptosis, proliferation differentiation. This has promoted us speculate involvement apoptosis pathway induced by TNF-related apoptosis-inducing ligand (TRAIL)/death receptor 5 (DR5). By using RT-PCR immunohistochemistry, here we show that expression TRAIL/DR5 reveals spatiotemporally yet partially cooperative...