A5101681260- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Wound Healing and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Breast Cancer Treatment Studies
- Cancer, Hypoxia, and Metabolism
- Wnt/β-catenin signaling in development and cancer
- Cancer-related molecular mechanisms research
- Endometrial and Cervical Cancer Treatments
- Traditional Chinese Medicine Analysis
- HER2/EGFR in Cancer Research
- Autophagy in Disease and Therapy
- Kruppel-like factors research
- Radiopharmaceutical Chemistry and Applications
- Cancer, Lipids, and Metabolism
- Cancer Mechanisms and Therapy
- Natural product bioactivities and synthesis
- Silk-based biomaterials and applications
- Burn Injury Management and Outcomes
- Toxin Mechanisms and Immunotoxins
- Phagocytosis and Immune Regulation
- Esophageal Cancer Research and Treatment
- Biological Research and Disease Studies
- Advanced Proteomics Techniques and Applications
First People's Hospital of Chongqing
2023-2024
West China Hospital of Sichuan University
2020-2023
Sichuan University
2020-2023
The Affiliated Yongchuan Hospital of Chongqing Medical University
2017-2022
Chongqing Medical University
2017-2022
Ezhou Central Hospital
2015-2020
Jinan University
2018-2019
First Affiliated Hospital of Jinan University
2019
Affiliated Hospital of Youjiang Medical University for Nationalities
2019
Southern University of Science and Technology
2018
Cancer progression is an intricate biological process profiled by not only unscheduled proliferation, but also altered metabolism mechanisms. In this article, we introduced a novel tumor suppressor gene (TSG), Zinc Finger DHHC-Type Containing 1 (ZDHHC1, known as ZNF377), frequently silenced due to epigenetic modification among various cancers, which exerts significant anti-tumor effects through metabolic regulation. Methods: Quantitative reversed-transcription PCR (qRT-PCR), reverse...
Palmitoylation is essential for the classic hallmarks of cancers through regulating protein stability and protein-protein interactions.ZDHHC22, as a well-known member palmitoyltrans-ferase family, its role has not been revealed in cancer.We found ZDHHC22 expression was significantly lower estrogen receptor (ER) negative breast cancer (BrCa) tissues cell lines, positively corelated with clinical prognosis BrCa patients.The might be caused by promoter methylation.ZDHHC22 inhibited...
Tumor chemo-resistance is a hallmark of malignant tumors as well the major cause poor survival rates in lung cancer. Transmembrane-4 L-six family member-1 (TM4SF1), an antigen that serves oncogene, mainly affects tumor invasion and metastasis. We investigated roles TM4SF1 non-small-cell cancer progression, particularly regulation chemo-sensitivity.TM4SF1 was silenced by small interfering RNA transfection.TM4SF1 expression cell lines tissues were determined Quantitative Real-time PCR. MTS,...
Increasing attention has been focused on cell type proteome profiling for understanding the heterogeneous multicellular microenvironment in tissue samples. However, current methods need large amounts of starting materials which preclude their application to clinical tumor specimens with limited access. Here, by seamlessly combining laser capture microdissection and integrated proteomics sample preparation technology SISPROT, specific types samples could be precisely dissected single...
Abstract Background Zinc-finger protein 471 (ZNF471) is a member of the Krüppel-associated box domain zinc finger (KRAB-ZFP) family. ZNF471 methylated in squamous cell carcinomas tongue, stomach and esophageal. However, its role breast carcinogenesis remains elusive. Here, we studied expression, functions, molecular mechanisms cancer. Methods We examined expression by RT-PCR qPCR. Methylation-specific PCR determined promoter methylation. Its biological functions related were assessed CCK-8,...
Abstract The inactivation of tumor-suppressor genes contributes heavily to oncogenesis. mutation TP53 has been well-studied and recognized as a major factor in the development tumors. Yet other means p53 not well-elucidated. We previously identified hypermethylated gene ZDHHC1 that suppresses tumor growth when expression was restored, but specific mechanism yet be found. protein product is an S-palmitoyltransferase we have substrate for ZDHHC1-mediated palmitoylation, specifically at C135,...
Abstract Kruppel like factor 15 (KLF15), a transcriptional belonging to the Kruppel-like (KLF) family of genes, has recently been reported as tumor suppressor gene in breast cancer. However, specific mechanisms by which KLF15 inhibits BrCa have not elucidated. Here we investigated role and mechanism triple-negative cancer (TNBC). expression methylation were detected RT-qPCR, RT-PCR methylation-specific PCR cell lines tissues. The effects on TNBC functions examined via various cellular...
In ischemia-reperfusion injury in ischemic stroke, mitophagy, which can remove damaged mitochondria, reduce cytotoxic damage, and enhance neurological recovery, is crucial. Jionoside A1 a substance found the traditional Chinese herb Rehmannia glutinosa, may have neuroprotective effects. The fundamental objective of this work was to find out A1's contribution ischemia/reperfusion stroke. oxygen-glucose deprivation/reperfusion (OGD-Rep) model right transient middle cerebral artery occlusion...
Background/Aims: Aberrant activation of the Wnt/β-catenin signaling pathway plays a key role in pathogenesis multiple tumors including digestive cancers. Recent studies have reported that Dickkopf-related protein 2 (DKK2) is epigenetically inactivated numerous types cancers and its gene products exhibit tumor-suppressive properties. However, biological functions underlying molecular mechanisms DKK2 colon carcinoma remains obscure. Methods: We examined expression tumor cell lines by RT-PCR...
Abstract Background and Aim The high mortality poor prognosis of hepatocellular carcinoma (HCC) have raised the public attention. Gene therapy is considered as a promising treatment option for cancer; thus, finding new therapeutic target HCC urgently needed. GATA4 tumor suppressor gene in multiple cancers, but its role unclear. In this study, we explored function HCC. Methods Reverse transcription–polymerase chain reaction quantitative polymerase were used to detect mRNA expression cells...
ErbB-2 gene encodes tyrosine kinase receptor p185(neu). Overexpression of erbB-2 plays a key role in tumorigenesis or progression such as breast cancer and ovarian cancer. Our previous study showed that ON-III (2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone) extracted from Traditional Chinese Medicine Cleistocaly xoperculatus dry flower could inhibit KDR phosphorylation tumor growth vivo. In this study, we reported repressed without reduced expression MDA-MB-453 cells. Activation...
<p>The aim of the study was to see antiproliferative and apoptotic effects ethanolic herbal extract Artemisia sieversiana against three human colon cancer (HT-29, HCT-15 COLO-205) cells. The cytotoxicity on these cell lines evaluated by MTT assay. Phase contrast fluorescence microscopy using acridine orange/ethidium bromide (AO/ETBR) staining employed investigate morphological alterations in COLO-205 cells extract. Flow cytometry instrument measured changes mitochondrial membrane...
Long non-coding RNAs are considered to be critical regulators of tumor progression. Tumor protein translationally controlled 1 antisense RNA (TPT1-AS1) was shown have an oncogenic role in cervical and ovarian cancer. The clinical significance biological function TPT1-AS1 gastric cancer (GC) not clear. In this study, we analyzed the expression GC tissues cell lines performed functional mechanistic analysis effects on proliferation, migration, invasion. determined 76 pairs vs. matched adjacent...
BACKGROUND:As we know, chemotherapy resistance is a critical factor leading to recurrence and metastasis of nonsmall-cell lung cancer (NSCLC). To clarify the key target potential mechanism gemcitabine (GEM) in NSCLC, selected Gene Expression Omnibus Data Set statistically analyzed parent cell group GEM-resistant group. Results showed that expression troponin C1, slow skeletal cardiac type (TNNC1) cells was higher than cells, which implies TNNC1 associated with GEM cells. MATERIAL AND...
Recent studies suggest that Hypocretin (HCRT, Orexin) are involved in stress regulation of depression through the hypothalamic-pituitary-adrenal (HPA) axis. However, molecular mechanism by which regulate neurobiological responses is unknown. Herein, effects chronic on epigenetic modification HCRT and its association with were explored regard to a potential role cancer progression. In study, Sprague Dawley (SD) rats used establish an animal model administrating n-nitrosodiethylamine (DEN)...
Abstract Background and Aim Hepatocellular carcinoma (HCC) urgently needs a marker for early diagnosis targeted treatment. C2orf40 has been identified as tumor suppressor gene in many cancers. However, the precise role regulatory mechanism by contribute to HCC remain elusive merit exploration. Methods Reverse‐transcription PCR, quantitative real‐time methylation‐specific PCR were used detect expression methylation of cell lines or tissues. The effects liver cancer cells examined via colony...
Our previous studies revealed that Jagged 2 (JAG2) is involved in the regulation of migration and invasion colon cancer cells without affecting cell proliferation. This study further explored specific mechanism by which JAG2 promotes colorectal cells.JAG2 mRNA expression different clinical stages normal intestinal tissues was detected quantitative PCR (QPCR). QPCR Western Blot were used to analyze differential protein between human tissue various cells. Co-expression status...
Hypertrophic-scar (HS) is the most common pathological healing phenomenon after trauma, especially deep burns. We aimed to investigate expression and role of microRNA-211-5p (miR-211-5p) in HS explore its underlying mechanism.Quantitative real-time polymerase chain reaction (qRT-PCR) was used detect miR-211-5p 15 cases tissues normal skin tissues, as well human hypertrophic scar fibroblasts (hHSFs) fibroblasts. At same time, cell counting kit-8 (CCK-8), scratch test, invasion flow cytometry...