Human multiple myeloma (MM) is a malignant and incurable B cell tumor. Zinc oxide nanoparticles (ZnO NPs) have been widely used in biomedical fields including anti-bacterial anti-tumor. However, the influence of ZnO NPs on MM cells still unclear. The present study aimed to investigate effect (a human myeloma-derived RPMI8226 line) death vitro underlying mechanism. morphology was characterized by transmission electron microscopy (TEM), inhibitory apoptotic monitored CCK-8 method an Annexin...

Acute myeloid leukemia (AML), as the most common malignancy of hematopoietic system, poses challenges in treatment efficacy, relapse, and drug resistance. In this study, we have utilized 151 RNA sequencing datasets, 194 DNA methylation 200 somatic mutation datasets from AML cohort TCGA database to develop a multi-omics stratification model. This model enables comparison prognosis, clinical features, gene mutations, immune microenvironment sensitivity across subgroups. External validation...

Acute myeloid leukemia (AML) is a hematological malignancy. Zinc oxide nanoparticles (ZnO NPs) and Luteolin are commonly used to fight cancer. In this study, we synthesized new complex: zinc nanoparticles-luteolin (ZnONPs-Lut) aimed investigate its effects on cell death in the AML line (MOLM-13) vitro elucidate underlying mechanisms. We assessed viability, quantified changes gene expression using real-time quantitative PCR (qRT-PCR), measured ferrous (Fe2+) content, glutathione (GSH)...

Zinc oxide nanoparticles (ZnO NPs) have exhibited excellent anti-tumor properties; the present study aimed to elucidate underlying mechanism of ZnO NPs induced apoptosis in acute myeloid leukemia (AML) cells by regulating mitochondrial division. THP-1 cells, an AML cell line, were first incubated with different concentrations for 24 hr. Next, expression Drp-1, Bcl-2, Bax mRNA, and protein was detected, effects on levels reactive oxygen species (ROS), membrane potential (Δψm), apoptosis, ATP...

Diffuse large B-cell lymphoma (DLBCL) is the most common type of lymphoma. Zinc oxide (ZnO) nanoparticles have excellent anti-tumor properties in biomedical field. The present study aimed to explore underlying mechanism by which ZnO induce toxicity DLBCL cells (U2932) via PINK1/Parkin-mediated mitophagy pathway. After U2932 were exposed various concentrations nanoparticles, cell survival rate, reactive oxygen species (ROS) generation, cycle arrest, and changes expression PINK1, Parkin, P62,...

This study aimed to investigate the dynamic effects of traditional Chinese medicine compound Longdan Xiegan Decoction (LXD) on inhibition Notch signaling pathway activation and T helper (Th) cell differentiation in rats with experimental autoimmune uveitis (EAU). Based a network pharmacology strategy, we conducted protein interaction analysis construct an active ingredient-disease treatment network. Gene Ontology (GO) enrichment Kyoto Encyclopedia Genes Genomes (KEGG) analyses were further...

Myopia is one of the most common vision defects worldwide. microRNAs can regulate target gene expression, influencing development diseases.To investigate alterations microRNA profiling in negative lens-induced myopia (NLIM) guinea pigs and to explore regulatory role occurrence myopia, we first established NLIM pig model after induction for 2 weeks. Further, isolated sclera purify total messenger RNA (mRNA) both fellow sclera. Using next generation sequencing technique bioinformatics...

Acute promyelocytic leukemia (APL) is a specific subtype of acute myelogenous (AML) characterized by the proliferation abnormal promyelocytes. Realgar, Chinese medicine containing arsenic, can be taken orally. Traditional physicians have employed realgar to treat APL for over thousand years. Therefore, may promising candidate treatment APL. Nevertheless, underlying mechanism behind therapy largely unclear. The present study aimed investigate effect on cell death in line (NB4) vitro and...

To reveal the potential mechanism of effect Chinese Herbal Medicine Fuzi on Aplastic anaemia (AA) according to network pharmacology approach and molecular docking. According Ultra High Performance Liquid Chromatography Mass Spectrometry (UHPLC-MS/MS), 146 chemical ingredients were obtained. By SwissADME online system analysis, a total 55 compounds such as Magnoflorine, Scutellarein, Luteolin Gingerol may be main active components 145 common targets related AA predicted. 17 MAPK1, AKT1 GRB2...

Acyl-CoA thioesterase (ACOT) plays a considerable role in lipid metabolism, which is closely related to the occurrence and development of cancer, nevertheless, its has not been fully elucidated acute myeloid leukemia (AML). To explore ACOT2 AML provide potential therapeutic target for AML, expression pattern ACOT was investigated based on TNMplot, Gene Expression Profiling Interactive Analysis (GEPIA), Cancer Cell Line Encyclopedia (CCLE) database, diagnostic value, prognostic clinical...

Background: Hedyotis diffusa Willd (HDW) is an herb that has been used empirically for treating cancer, and its antileukemic effect confirmed by laboratory evidence. This study aimed to explore the underlying mechanism which HDW active compound exert effects on acute myeloid leukemia (AML) through in silico analyses combined with experimental validation. Methods: The targets of compounds were collected from database intersected AML targets. Based these data, a protein-protein interaction...

Introduction: nutritional status can affect the treatment of hospitalized patients, and malnutrition even lead to death. However, patients with aplastic anemia (AA) is unclear. Objective: assess body mass index (BMI) ≥ 24 kg/m2 (high BMI group) < (low group), compare consistency between different screening tools. Methods: from January 2016 December 2020 were collected. We used combined generated by Nutritional Risk Index (NRI), Prognostic (PNI), Control Status (CONUT) Instant Assessment...

Topic: 3. Acute myeloid leukemia - Biology & Translational Research Background: promyelocytic (APL) is a specific subtype of acute myelogenous (AML) characterized by the proliferation abnormal promyelocytes. Realgar, Chinese medicine containing arsenic, can be taken orally. Traditional physicians have employed realgar to treat APL for over thousand years. Therefore, may promising candidate treatment APL. Nevertheless, underlying mechanism behind therapy largely unclear. Aims: The present...

Abstract Myopia is one of the most common vision defects worldwide. microRNAs can regulate target gene expressions, influencing development diseases. In order to investigate alterations profiling in negative lens-induced myopia (NLIM) guinea pigs and explore regulatory role occurrence myopia, we first established NLIM pig model after induction for 2 weeks. Further, isolated sclera purify total messenger RNA (mRNA) both fellow sclera. Using next generation sequencing technique bioinformatics...

Abstract Background: Myopia is the most common vision defects and leading cause of visual impairment worldwide. microRNAs can regulate target gene expressions, influencing development diseases. However, roles differentially expressed in myopia lens-induced (LIM) guinea pigs remain still unclear. Methods: We first established a negative LIM pig model after induction for 2 weeks. Further, we isolated sclera to purify total messenger RNA (mRNA) both fellow sclera. Using next generation...

Abstract Myopia is one of the most common vision defects worldwide. microRNAs can regulate target gene expressions, influencing development diseases. In order to investigate alterations profiling in negative lens-induced myopia (NLIM) guinea pigs and explore regulatory role occurrence myopia, we first established NLIM pig model after induction for 2 weeks. Further, isolated sclera purify total messenger RNA (mRNA) both fellow sclera. Using next generation sequencing technique bioinformatics...

Abstract Myopia is one of the most common vision defects worldwide. microRNAs can regulate target gene expression, influencing development diseases. In order to investigate alterations microRNA profiling in negative lens-induced myopia (NLIM) guinea pigs and explore regulatory role occurrence myopia, we first established NLIM pig model after induction for 2 weeks. Further, isolated sclera purify total messenger RNA (mRNA) both fellow sclera. Using next generation sequencing technique...