A5066290842- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Antibiotic Resistance in Bacteria
- Pneumocystis jirovecii pneumonia detection and treatment
- Streptococcal Infections and Treatments
- Antibiotic Use and Resistance
- Infective Endocarditis Diagnosis and Management
- Antimicrobial Resistance in Staphylococcus
- Neonatal and Maternal Infections
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Nail Diseases and Treatments
- Respiratory viral infections research
- Nosocomial Infections in ICU
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- Urinary Tract Infections Management
- Enterobacteriaceae and Cronobacter Research
- Gallbladder and Bile Duct Disorders
- Microbial infections and disease research
- Neonatal and fetal brain pathology
- Otolaryngology and Infectious Diseases
- Dermatology and Skin Diseases
- Vibrio bacteria research studies
- Leprosy Research and Treatment
University of Hassan II Casablanca
2015-2023
Centre Hospitalier Universitaire Ibn Rochd
2013-2023
Invasive pneumococcal disease remains an important health priority owing to increasing incidence caused by pneumococci expressing non-vaccine serotypes. We previously defined 621 Global Pneumococcal Sequence Clusters (GPSCs) analysing 20 027 isolates collected worldwide and from published genomic data. In this study, we aimed investigate the lineages behind predominant serotypes, mechanism of serotype replacement in disease, as well major contributing invasive post-vaccine era their...
The purpose of this study was to compare the incidence rate invasive pneumococcal disease, rates antibiotic resistance and serotype distribution among children ≤5 years old before after PCVs introduction in Casablanca, Morocco.This conducted at Ibn Rochd University Hospital Centre Casablanca during two periods encompassing pre-and post-implementation PCVs, respectively from January 2007 October 2010 2011 December 2014. All non-duplicate S. pneumoniae isolates recovered were included.There...
Multidrug-resistant Streptococcus pneumoniae emerge through the modification of core genome loci by interspecies homologous recombinations, and acquisition gene cassettes. Both occurred in otherwise contrasting histories antibiotic-resistant S. lineages PMEN3 PMEN9. A single clade spread globally, evading vaccine-induced immunity frequent serotype switching, whereas locally circulating PMEN9 clades independently gained resistance. repeatedly integrated Tn 916 -type 1207.1 elements,...
Pneumococcal disease outbreaks of vaccine preventable serotype 4 sequence type (ST)801 in shipyards have been reported several countries. We aimed to use genomics establish any international links between them.Sequence data from ST801-related outbreak isolates Norway (n = 17), Finland 11) and Northern Ireland 2) were combined with invasive pneumococcal surveillance the respective countries, genomes an collection 41 > 40,000), totalling 106 genomes. Raw mapped recombination excluded before...
ABSTRACT Worldwide spread of antibiotic resistance in Streptococcus pneumoniae is a major problem. However, data from West and North African countries are scarce. To study the level compare situations different cities, prospective was conducted Abidjan (Ivory Coast), Casablanca (Morocco), Dakar (Senegal), Tunis (Tunisia), 1996 to 1997. The resistances eight antibiotics 375 isolates were studied by E test, results interpreted using breakpoints recommended National Committee for Clinical...
Pertussis, a vaccine preventable disease, is still responsible of significant morbidity and mortality around the world, mostly in newborns. The aim present study was (1) to introduce pertussis surveillance major pediatric hospital Casablanca (2) analyze prevalence among children under 14 years age their entourage Casablanca, Morocco. This prospective non-case controlled study, including suspected Pertussis admitted at Abderrahim Harouchi Pediatric Hospital from January 2013 June 2015....
The aim of this prospective study in Morocco was to investigate the causes invasive bacterial diseases children order inform antibiotic therapy and vaccine choices. Of 238 aged < or = 5 years admitted Children's Hospital Casablanca for over a 12-month period, 185 were diagnosed with infection: 76 had chest-X-ray-confirmed pneumonia, 59 meningitis 50 sepsis. Streptococcus pneumoniae most common pathogen identified (n 24), followed by Neisseria meningitidis 18, all group B) Haemophilus...
Streptococcus pneumoniae is a major cause of morbidity and mortality worldwide, especially among children the elderly. The ability to effectively treat pneumococcal infection has been compromised due acquisition antibiotic resistance, particularly β-lactam drugs. This study aimed describe prevalence molecular evolution penicillin non-susceptible S. (PNSP) isolated from invasive diseases before after conjugate vaccine implementation in Casablanca, Morocco. Isolates were obtained Microbiology...
Introduction: Acute bacterial meningitis is one of the most severe infectious diseases. Rapid, accurate, and inexpensive diagnosis crucial for patient management. This study describes a duplex real-time (RT) PCR assay detection Neisseria meningitidis Streptococcus pneumoniae in cerebrospinal fluid (CSF) using SYBR Green-based RT-PCR method coupled with melting curve analysis. Methodology: We used analysis to detect S. N. CSF samples. The sensitivity, specificity, limit were determined. gold...
The species Bordetella trematum was first described in 1996. Currently only eleven cases were published. We describe the case of issued from bacteremia with a patient who has severe burns Morocco. identification not possible by conventional microbiological methods where resort to 16S ARNr sequencing. use molecular methods, including sequencing ARNr, is currently an essential complementary tool identify pathogens.
Objective: To analyze trends of antibiotic resistance rates in Streptococcus pneumoniae from 1998 to 2008 Casablanca (Morocco). Methods: The levels 955 consecutive nonduplicate isolates were studied using E test and disc diffusion methods. Results interpreted following Clinical Laboratory Standards Institute guidelines (2005). Analysis was done according three periods (1998–2001; 2002–2005; 2006–2008), age, site infection. Results: Penicillin nonsusceptibility (PNS) increased significantly...
This study aimed to investigate the nature of amino acid motifs found in PBPs Streptococcus pneumoniae isolates invasive diseases from pediatric patients at Casablanca, Morocco. Five penicillin-susceptible (PSSP), ten penicillin-intermediate (PISP), and fifteen penicillin-resistant S. (PRSP) were studied by PCR–RFLP DNA sequencing pbp1a, − 2b, 2x genes. There no changes conserved PBP1a, PBP2b PBP2x for PSSP strains. Substitution close PBP1a all PRSP six/five PISP. Analysis showed that but...
Abstract Objectives We reported tet(S/M) in Streptococcus pneumoniae and investigated its temporal spread relation to nationwide clinical interventions. Methods whole-genome sequenced 12 254 pneumococcal isolates from 29 countries on an Illumina HiSeq sequencer. Serotype, multilocus ST antibiotic resistance were inferred genomes. An SNP tree was built using Gubbins. Temporal reconstructed a birth–death model. Results identified 131 none carried other known tet genes. Tetracycline...
Meningococcal epidemiology may change unpredictably, and typing of Neisseria meningitidis isolates is crucial for the surveillance invasive meningococcal disease (IMD). Few data are available regarding in countries North Africa. We aimed to explore from Casablanca region Morocco. used whole-genome sequencing (WGS) characterize 105 this during period 2011 2016. Our showed that majority (n = 100) belonged serogroup B. Genotyping indicated most 62) sequence type 33 clonal complex 32. The also...
Abstract Background In recent decades, there has been a marked increase in the number of reported cases pertussis around world, and continues to be frequently occurring disease despite an effective childhood vaccination. This study aims determine role household contacts children diagnosed with Casablanca Morocco. Methods From November 2015 October 2017, suspected whooping cough that consulted Ibn Rochd University hospital at their were enrolled study. Nasopharyngeal (NP) samples analyzed by...
Blood cultures are the best diagnostic tool for detection of bacteremia. However, false positive results may lead to confusion about antibiotic regimens, putting lives patients at risk. The main purpose this study was assess prevalence coagulase negative Staphylococci (CoNS) as well Corynebacterium spp and Bacillus in bags blood culture analyzed microbiology laboratory Ibn-Rochd University Hospital Casablanca. This evaluated according various Departments over year 2016.We conducted a...
Abstract Background Streptococcus pneumoniae (S. pneumoniae) , remains a major cause of mortality and morbidity worldwide. The objective this study was to determine the trends invasive pneumococcal diseases (IPD) in adult elderly population Casablanca (Morocco) before after introduction conjugate vaccine (PCV) by determining distribution serotypes antibiotic resistance profile isolated strains. Method proposed is retrospective laboratory-based surveillance IPD hospitalized (15–59 years old)...
Objectif : L’objectif du travail est de déterminer l’incidence des bactériémies associées aux soins (BAS) en milieu réanimation Centre hospitalier universitaire (CHU) Ibn Rochd Casablanca-Maroc, la fréquence bactéries isolées et leur résistance antibiotiques. Méthodes Il s’agit d’une étude longitudinale prospective menée entre mai juillet 2015, auprès patients hospitalisés CHU Rochd. La définition bactériémie adoptée était celle Comité technique infections nosocomiales liées (CTINILS)....
Introduction: the laboratory diagnosis of tuberculosis (TB) relies mainly on conventional techniques. However, it either lacks sensitivity or is time-consuming. This study aims to evaluate use real-time polymerase chain reaction (PCR) targeting IS6110 for Mycobacterium Complex (MTBC) in routine TB our laboratory.
Surveillance of invasive meningococcal diseases (IMD) must be carried out regularly and continuously in order to detect the emergence strains reduced susceptibility antibiotics for therapeutic prophylactic use appearance new clones. Molecular-typing approaches allow reliable traceability powerful epidemiological analysis. This is an study Neisseria meningitidis causing meningitis Casablanca, Morocco. The grouping was confirmed by PCR mainly on isolates from cerebrospinal fluid (CSF). A total...