A5020269627- Antifungal resistance and susceptibility
- Antimicrobial Resistance in Staphylococcus
- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Bacteriophages and microbial interactions
- Bacterial Identification and Susceptibility Testing
- Plant Pathogenic Bacteria Studies
- Antibiotic Resistance in Bacteria
- Plant-Microbe Interactions and Immunity
- Pharmaceutical and Antibiotic Environmental Impacts
- Mycobacterium research and diagnosis
- Dental Health and Care Utilization
- Listeria monocytogenes in Food Safety
- SARS-CoV-2 and COVID-19 Research
- Helminth infection and control
- Essential Oils and Antimicrobial Activity
- Antibiotics Pharmacokinetics and Efficacy
- Rheumatoid Arthritis Research and Therapies
- Mycotoxins in Agriculture and Food
- Salivary Gland Disorders and Functions
- Fungal Biology and Applications
- Microbial Natural Products and Biosynthesis
- Medical Device Sterilization and Disinfection
- SARS-CoV-2 detection and testing
- Spondyloarthritis Studies and Treatments
Medical University of Vienna
2019-2025
FH Campus Wien
2024
Introduction/Objectives An increase in antifungal resistant Candida strains has been reported recent years. The aim of this study was to detect mutations resistance genes azole-resistant, echinocandin-resistant or multi-resistant using next generation sequencing technology, which allows the analysis multiple mechanisms a high throughput setting. Methods Forty clinical isolates (16 C. albicans and 24 glabrata strains) with MICs for azoles echinocandins above EUCAST breakpoint were examined....
Topical antiseptics are crucial for preventing infections and reducing transmission of pathogens. However, commonly used antiseptic agents have been reported to cause cross-resistance other antimicrobials in bacteria, which has not yet described yeasts. This study aims assess the vitro efficacy against clinical reference isolates Candida albicans Nakaseomyces glabratus, whether prolonged exposure promotes development antifungal (cross)resistance. A high-throughput approach resistance was...
Candida auris is a novel and emerging pathogenic yeast which represents serious global health threat. Since its first description in Japan 2009, it has been associated with large hospital outbreaks all over the world often resistant to more than one antifungal drug class. To date, five C. isolates have detected Austria. Morphological characterization susceptibility profiles against echinocandins, azoles, polyenes pyrimidines, as well new antifungals ibrexafungerp manogepix, were determined....
Infections due to Candida species represent a serious threat healthcare facilities. Only few classes of antifungal agents are available, and the rapid emergence (multi)drug resistance eliminates effective treatment options for successful patient outcome. Topically applied antiseptics may suitable tool infection control local therapy. This study aimed investigate in vitro efficacy widely used antiseptic octenidine (OCT) against clinical isolates emerging azole-, echinocandin- multi-resistant...
Abstract Cefiderocol, a novel siderophore cephalosporin, demonstrates promising in vitro activity against multidrug-resistant Gram-negative bacteria, including carbapenemase-producing strains. Nonetheless, only few reports are available regarding the acquisition of resistance clinical settings, primarily due to its recent usage. This study aimed investigate cefiderocol using an development model gain insights into underlying molecular mechanisms. Cefiderocol susceptible reference strains (...
Invasive infections caused by filamentous fungi constitute a leading cause of morbidity and mortality in immunocompromised patients. Rapid reliable identification is essential for the early initiation appropriate treatment. In present study, 230 isolates identified conventional methods were investigated using MALDI-TOF MS (Bruker Daltonics, Bremen, Germany) combination with Filamentous Fungi Library 3.0 provided manufacturer. Three different sample preparation applied as recommended...
Abstract Background Gingivitis is the most common form of periodontal disease among children and adolescents associated with disrupted host–microbiome homeostasis. Family an important factor influencing prevalence gingivitis. In present study, we investigated salivary microbiome, oral hygiene habits, level myeloid‐related protein (MRP)‐8/14 in aged 7–12 years gingivitis, periodontally healthy children, their mothers. Methods This study included 24 gingivitis (including four sibling pairs) 22...
The increasing prevalence of methicillin-resistant Staphylococcus aureus (MRSA) strains resistant to non-beta-lactam antimicrobials poses a significant challenge in treating severe MRSA bloodstream infections. This study explores resistance development and mechanisms isolates, especially after the first dalbavancin-resistant strain our hospital 2016. investigated 55 isolates (02/2015–02/2021) from University Hospital Medical Vienna, Austria. MICs dalbavancin, linezolid, daptomycin were...
Abstract Purpose Due to an increasing incidence of invasive fungal infections, the availability reliable diagnostic tools for fast detection a wide spectrum pathogens is vital importance. In this study, we aimed conduct extensive clinical evaluation recently published in-house panfungal PCR assay on samples from suspected infections. Methods Overall 265 232 patients with disease (96 deep airway samples, 60 sterile fluids, 50 tissue biopsies, and 59 blood samples) were included. All underwent...
Peritonitis remains the major infectious complication in setting of peritoneal dialysis (PD). Despite known only moderate pathogenicity, most frequently detected pathogens PD-related peritonitis are surprisingly coagulase-negative staphylococci. However, this could be explained, at least part, by Staphylococcus aureus small colony variants (SCVs) induced PD fluids (PDFs) and misidentified routinely used microbiological methods. Bacteria were exposed to commonly PDFs various regimens designed...
Candida-associated infections put a significant burden on western healthcare systems. Development of (multi-)resistant fungi can become untreatable and threaten especially vulnerable target groups, such as the immunocompromised.We assessed antifungal susceptibility explored possible influence factors clinical Candida isolates collected from Austrian hospitals between 2007 2016.Thousand three hundred sixty spp. isolated blood cultures were subjected to testing (AFST) in liquid-handling aided...
Invasive Staphylococcus aureus infections are associated with a high burden of disease, case fatality rate and healthcare costs. Oxazolidinones such as linezolid tedizolid considered potential treatment choices for conditions involving methicillin resistance or penicillin allergies. Additionally, they being investigated inhibitors toxins in toxin-mediated diseases. In this study, were evaluated an vitro development model induction S. aureus. Whole genome sequencing was conducted to elucidate...
Antifungal susceptibility testing is important in guiding patient therapy due to an increasing number of resistant Candida isolates. In the clinical strain collection Austrian resistance report (AURES), a high micafungin-resistant C. albicans isolates (18.2% 49/269) was detected seven different centres Austria from 2011–2016. Most these showed micafungin MIC value that just above breakpoint (CB) established by EUCAST (0.016 mg/L). The aim this study analyse whether strains showing 1–2...
In the past, human microbiome has consistently been associated with rheumatoid arthritis (RA) and disease activity. Here, we investigate antimicrobial activity of disease-modifying antirheumatic drugs (DMARDs) against typical representatives oral microflora that have RA.DMARDs were screened for bacteria are pathogenesis and/or frequently isolated from patients RA. Screening was done by an agar diffusion assay minimum inhibitory concentrations (MICs) active substances then determined broth...
Abstract Purpose: The increasing prevalence of methicillin-resistant Staphylococcus aureus (MRSA) strains resistant to non-beta-lactam antimicrobials poses a significant challenge in treating severe MRSA bloodstream infections. This study explores resistance development and mechanisms isolates, especially after the first dalbavancin-resistant strain our hospital 2016. Methods: investigated 55 isolates (02/2015–02/2021) from University Hospital Medical Vienna, Austria. MICs dalbavancin,...
Amid the global challenges posed by COVID-19 pandemic, unraveling genomic intricacies of SARS-CoV-2 became crucial. This study explores viral evolution using an innovative high-throughput next-generation sequencing (NGS) approach. By taking advantage nasal swab and mouthwash samples from patients who tested positive for across different geographical regions during sequential infection waves, our applied a targeted enrichment protocol pooling strategy to increase detection sensitivity. The...
Reprocessing failure of endoscopes may result in outbreaks serious infections vulnerable patients caused by Gram-negative bacteria.
Plant-derived foods are potential vehicles for microbial antibiotic resistance genes (ARGs), which can be transferred to the human microbiome if consumed raw or minimally processed. The aim of this study was determine prevalence and amount clinically relevant ARGs mobile genetic elements (MGEs) in differently processed smoothies (freshly prepared, cold-pressed, pasteurized high-pressure processed) fresh produce samples (organically conventionally cultivated) assess health hazards associated...