Renal fibrosis, which is characterized by excessive extracellular matrix (ECM) accumulation in the renal tubulointerstitium, can lead to chronic kidney disease (CKD). The role of microfiber-associated protein 4 (MFAP4), an ECM that interacts with elastin and collagen, fibrosis has not been investigated. aim this study was examine MFAP4 pathogenesis underlying mechanism using vivo vitro models. MFAP4−/− mice were subjected unilateral ureteral obstruction (UUO) elucidate vivo. Compared...

Printing three-dimensional (3D) scaffolds with suitable mechanical cues is an effective strategy for guiding tissue regeneration by inducing cell migration and growth. Nevertheless, many studies considered only one type of cue 3D engineering scaffold fabrication, such as topological cues, which insufficient. To realize durotaxis- topotaxis-orchestrated guidance on migration, a printed scaffold/hydrogel composite was fabricated in this study. The porous provided (topotaxis), the combined...

Background: Abnormal expression of microRNAs (miRNAs) is closely related to glioma, which one the most common malignant brain tumors. The current study identify key miRNAs involved in pathogenesis glioma and discover novel therapeutic targets for this disease. Materials Methods: Total RNA was extracted from tissues 100 patients. microRNA microarray northern blot were used detect changes 7 pairs specimens. Relative expressions miR-23a validated by real-time reverse transcription polymerase...

Objectives: Acute liver failure (ALF) produces cerebral dysfunction and edema, mediated in part by elevated ammonia concentrations, often leading to coma death. The pathophysiology of edema ALF is incompletely understood. In vitro models the effects have predominately consisted dissociated astrocyte cultures or acute brain slices. We describe a stable long-term culture model incorporating both neural glial elements three-dimensional tissue structure offering significant advantages study...

Substantial mucosa defects in gastrointestinal tracts are difficult to repair, causing high mortality. Existing artificial studies overlooked the natural surface texture and paracrine action between epithelial cells fibroblasts, leading an incomplete repair of mucosa. Here, a Janus film with exterior having ridge-like interior flat mimic anatomical pattern digestive is obtained through cryogenic electrospinning (e-spinning). Inspired by textures, biopolymer nanofiber assemblies employed...

Abstract Cryptotanshinone (CTS) is a natural compound from the Chinese herb Salvia miltiorrhiza . Previous studies demonstrated that CTS possesses anti‐apoptotic and anti‐inflammatory properties. However, its effects underlying mechanism on renal ischaemia reperfusion (IR) injury remain unknown. In present study, we investigated of IR potential mechanisms. Mice were randomized into four groups as follows: (a) sham operation + vehicle, (b) CTS, (c) (d) CTS. The CTS‐treated group injected...

Abstract Renal fibrosis acts as a clinical predictor in patients with chronic kidney disease and is characterized by excessive extracellular matrix (ECM) accumulation. Our previous study suggested that mindin can function mediator for liver steatosis pathogenesis. However, the role of renal remains obscure. Here, tumour necrosis factor (TGF)‐β‐treated HK‐2 cells global knockout mouse were induced ischaemia reperfusion injury (IRI) to test relationship between fibrosis. In vitro,...

Adipose-derived stem cells (ADSCs), which are present in most organs and tissues, were evaluated as a novel medium for cell therapy. In this study, we investigated the effects underlying mechanisms of ADSCs bladder tumor (BT) cells. SV-HUC, T24, EJ cultured with conditioned from (ADSC-CM). We observed that routine culture, significantly inhibited proliferation T24 dose-dependent manner. addition, ADSC-CM attenuated viability Cell cycle analysis indicated was capable inducing S phase arrest...

Cholangiocarcinoma (CCA) is a malignant biliary tract tumor with an extremely poor prognosis. There urgent demand to explore novel therapeutic strategies. L-fucose has been confirmed participate in anti-inflammation and antitumor activities. However, the effect of on progression CCA not well investigated. This study aimed determine whether induced inhibition its possible mechanism.The anti-growth activity was determined using Cell Counting Kit-8 assay, colony formation assays, Annexin...

BackgroundWe undertook a study to determine whether propofol pretreatment may protect human proximal renal tubular epithelial (HK-2) cells against anoxia-reoxygenation injury and the role of p38 mitogen-activited protein kinase (p38MAPK).

This study aimed to examine the effects of Lefty A protein on transforming growth factor-β1 (TGF-β1)-mediated apoptosis in human renal tubular epithelial cells (HK-2). HK-2 were transfected with gene induce secretion endogenous protein. Following exposure recombinant TGF-β1 (10 ng/ml), p-Smad2/3 levels examined by western blot analysis, and cellular was detected flow cytometry 6, 12, 24 48 h following treatment. Coculture resulted a significant increase rate cell apoptosis, which attenuated...

Abstract Background Acute renal injury (AKI) secondary to ischemia reperfusion (IR) continues be a significant perioperative problem and there is no effective treatment. Mindin belongs the mindin/F-spondin family involves in inflammation, proliferation, cell apoptosis. Previous studies have explored biological functions of mindin liver brain ischemic injury, but its role AKI unknown. Method To investigate whether has pathogenic role, knockout (KO) wild-type (WT) mice were used establish IR...

<title>Abstract</title> Aims: This study aimed to investigate the expression and role of Signaling Lymphocytic Activation Molecule Family Member 8 (SLAMF8) in Alzheimer’s disease (AD), particularly its interaction with NINJ2 TLR4/NF-κB signaling pathway. Methods: SLAMF8 levels AD models were analyzed using Gene Expression Omnibus (GEO) database. Validation was conducted through RT-PCR Western blot Aβ₁₋₄₂-exposed SH-SY5Y cells, LPS-exposed HMC3 APP/PS1 transgenic mice. The between...

Electrospinning (e-spinning) has been widely applied to fabricate flat films accumulated by microfibers for tissue engineering. In order acquire an uneven surface morphology, two methods have traditionally. The first uses a designed receiving substrate, which is stable, but suppresses the flexibility. second dual solvents achieve bimodal distribution of fiber diameter. However, diameter causes inhomogeneity. To solve these challenges, cryogenic electrospinning, using substrate and single...

To solve the ATM replenishment optimization problem, we conducted in-depth research and analysis on it, established a counterfeit model, proposed an improved algorithm to it. Combine global search capability of Genetic Algorithm (GA), excellent convergence Particle Swarm (PSO) local Simulated Annealing (SA) propose hybrid frame. actual production case bank's planning verify effectiveness algorithm, experimental results show that is effective in solving mathematical model useful for problem.

Objective To investigate the effects of glutamine on changes nuclear factor (NF) -KB and lung pathology in acute injury. Methods Forty SD rats underwent injection lipopolysaccharide (LPS) 5 mg/kg into femoral vein, were randomly divided 4 equal groups; Group B; undergoing 0. 75 g/kg vein Ih before LPS injection, C simultaneously, D after E without injection. Another 10 normal saline to be used as controls (Group A). Four hours killed with their lungs taken out. RT-PCR was detect level...