A5018156686- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Respiratory viral infections research
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
Shanghai Public Health Clinical Center
2019-2025
The emergence and spread of antibiotic resistance pose serious environmental health challenges. Attention has been drawn to phage therapy as an alternative approach combat with immense potential. However, one the obstacles is resistance, it can be acquired through genetic mutations, followed by consequences phenotypic variations. Therefore, understanding mechanisms underlying phage-host interactions will provide us greater detail on how optimize therapy. In this study, three lytic phages...
ABSTRACT The marine ecosystem is characterized by a rich diversity of bacterial hosts and their phages. propagation phages primarily limited ability to adsorb host cells further challenged various defense mechanisms. To fully realize the potential phage therapy in aquaculture, comprehensive understanding phage-host interactions regulation essential. In this study, we isolated novel Myoviridae phage, BX-1, capable infecting Vibrio alginolyticus AP-1, its resistant mutants. We elucidated...
ABSTRACT N -acetylcysteine (NAC) and ambroxol hydrochloride (AMB) are commonly prescribed alongside antibiotics to alleviate sputum retention in lower respiratory tract infections, which often caused by bacterial pathogens. With the rising threat of antibiotic resistance, phage therapy has emerged as a promising alternative alongside. However, no studies have explored potential interactions between phages these mucoactive agents despite their frequent concurrent use during therapy....
ABSTRACT Quorum sensing (QS) plays a crucial role in regulating key traits, including the upregulation of phage receptors, which leads to heightened susceptibility Pseudomonas aeruginosa . As result, higher cell densities typically increase risk invasions. This has led speculation that bacteria may have evolved strategies counterbalance this increased susceptibility. Additionally, non-synonymous mutations LasR, master regulator QS, are common among cystic fibrosis patients, but impact these...
Klebsiella pneumoniae is a dominant cause of community-acquired and nosocomial infections, specifically among immunocompromised individuals. The increasing occurrence multidrug-resistant (MDR) isolates has significantly impacted the effectiveness antimicrobial agents. As antibiotic resistance becoming increasingly prevalent worldwide, use bacteriophages to treat pathogenic bacterial infections recently gained attention. Elucidating details phage-bacteria interactions will provide insights...
Filamentous phages are ubiquitously distributed in the global oceans. However, little is known about their biological contribution to host's genetic and phenotypic diversity. In this study, a filamentous phage, Vaf1, was isolated characterized from emerging marine pathogen strain Vibrio alginolyticus AP-1. We explored effects of resident phage Vaf1 on host physiology under diverse conditions by precisely deleting entire Vaf1. Our results demonstrate that presence significantly increased...
Pseudomonas aeruginosa is a common opportunistic human pathogen. With the emergence of multidrug-resistant (MDR) clinical infection P. aeruginosa, phage therapy has received renewed attention in treating infections. Moreover, detailed understanding host receptor lytic crucial for selecting proper phages therapy. Here, we describe characterization bacteriophage L5 with double-stranded DNA genome 42,925 bp. The genomic characteristics indicate that belonging to subfamily Autographivirinae. In...
Prophage 919TP is widely distributed among Vibrio cholera and induced to produce free φ919TP phage particles. However, the interactions between prophage φ919TP, particle, its host remain unknown. In particular, resistance mechanisms potential fitness trade-offs, resulting from resistance, are unresolved. this study, we examined a 919TP-deleted variant of V. cholerae interaction with modified lytic (φ919TP cI-). Specifically, phage-resistant mutant was isolated by challenging prophage-deleted...