- Pineapple and bromelain studies
- Plant tissue culture and regeneration
- Garlic and Onion Studies
- Plant Gene Expression Analysis
- Advanced biosensing and bioanalysis techniques
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant biochemistry and biosynthesis
- melanin and skin pigmentation
- Studies on Chitinases and Chitosanases
- Transgenic Plants and Applications
- Bioinformatics and Genomic Networks
- Phytochemical compounds biological activities
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
- Plant Physiology and Cultivation Studies
- Advanced Proteomics Techniques and Applications
- Nuts composition and effects
- Cocoa and Sweet Potato Agronomy
- Horticultural and Viticultural Research
- Photosynthetic Processes and Mechanisms
- Gene expression and cancer classification
- Antioxidant Activity and Oxidative Stress
South China Agricultural University
2016-2025
Ministry of Agriculture and Rural Affairs
2020-2025
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2024
Ji Hua Laboratory
2020-2023
Guangdong Ocean University
2018
Abstract The rapid development of high-throughput sequencing (HTS) techniques has led biology into the big-data era. Data analyses using various bioinformatics tools rely on programming and command-line environments, which are challenging time-consuming for most wet-lab biologists. Here, we present TBtools (a T oolkit B iologists integrating biological data handling ), a stand-alone software with user-friendly interface. toolkit incorporates over 100 functions, designed to meet increasing...
WRKY proteins comprise a large family of transcription factors that play important roles in many aspects physiological processes and adaption to environment. However, little information was available about the genes pineapple (Ananas comosus), an tropical fruits. The recent release whole-genome sequence allowed us perform genome-wide investigation into organization expression profiling genes.In present study, 54 (AcWRKY) were identified renamed on basis their respective chromosome...
The MYB proteins comprise one of the largest families plant transcription factors, which are involved in various physiological and biochemical processes. Pineapple (Ananas comosus) is three most important tropical fruits worldwide. completion pineapple genome sequencing provides a great opportunity to investigate organization evolutionary traits genes at genome-wide level.In present study, total 94 R2R3-MYB were identified further phylogenetically classified into 26 subfamilies, as supported...
Plums are one of the most economically important Rosaceae fruit crops and comprise dozens species distributed across world. Until now, only limited genomic information has been available for genetic studies breeding programs plums. Prunus salicina, an diploid plum species, plays a predominant role in modern commercial production. Here we selected P. salicina whole-genome sequencing present chromosome-level genome assembly through combination Pacific Biosciences sequencing, Illumina Hi-C...
Abstract Red fruit peel is an attractive target for pineapple breeding. Various accessions with distinct red coloration patterns exist; however, the precise molecular mechanism accounting these differences remains unknown, which hinders breeding process from combining high quality peel. In this study, we characterized a transcription factor, AcMYB266, preferentially expressed in and positively regulates anthocyanin accumulation. Transgenic pineapple, Arabidopsis, tobacco plants...
AP2/ERF (APETALA2/Ethylene Responsive Factor) transcription factors, a class of plant-specific play pivotal role in plant growth, development, metabolism, and stress response. The pineapple (Ananas comosus (L.) Merr.), perennial fruit, belongs to the Bromeliaceae family. It is an economically important crop worldwide, which consumed as fresh canned fiber source, even pharmaceutical raw material. We identified 75 AcoAP2/ERF genes genome, with four manually curated. They were distributed...
Leaf coloration is one of the most important and attractive characteristics Ananas comosus var. bracteatus. The chimeric character not stable during in vitro tissue culturing. Many regenerated plants lost economic values for loss leaves. In order to reveal molecular mechanisms involved albino phenotype leaf cells, physiological transcriptional differences between complete white (CWh) green (CGr) cells A. bracteatus were analyzed. A total 1,431 differentially expressed unigenes (DEGs) CGr CWh...
The pineapple (Ananas comosus) is cold sensitive. Most cultivars are injured during winter periods, especially in sub-tropical regions. There a lack of molecular information on the pineapple's response to stress. In this study, high-throughput transcriptome sequencing and gene expression analysis were performed plantlets cold-tolerant genotype cultivar 'Shenwan' before after treatment. A total 1,186 candidate responsive genes identified, their credibility was confirmed by RT-qPCR. Gene set...
ABSTRACT Pineapple is the third most crucial tropical fruit worldwide and available in five varieties. Genomes of different pineapple varieties have been released to date; however, none them are complete, with all exhibiting substantial gaps representing only two This significantly hinders advancement breeding efforts. In this study, we sequenced genomes three varieties: a wild variety, fiber globally cultivated edible variety. We constructed first gap‐free reference genome (Ref) for...
Background Ananas comosus var. bracteatus (Red Pineapple) is an important ornamental plant for its colorful leaves and decorative red fruits. Because of complex genome, it difficult to understand the molecular mechanisms involved in growth development. Thus high-throughput transcriptome sequencing necessary generate large quantities transcript sequences purpose gene discovery functional genomic studies. Results The was sequenced by Illumina paired-end technology. We obtained a total 23.5...
Pineapple is the only commercially grown fruit crop in Bromeliaceae family and has significant agricultural, industrial, economic, ornamental value. GRF (growth-regulating factor) proteins are important transcription factors that have evolved seed plants (embryophytes). They contain two conserved domains, QLQ (Gln, Leu, Gln) WRC (Trp, Arg, Cys), regulate multiple aspects of plant growth stress response, including floral organ development, leaf growth, hormone responses. The been...
Ananas comosus var. bracteatus is a colorful plant used as cut flower or landscape ornamental. The unique foliage color of this includes both green and red leaves and, trait interest, deserves investigation. In order to explore the pigments behind section chimeric leaves, parts were sampled analyzed at phenotypic, cellular molecular levels in study.The CIELAB results indicated that a* values L* samples had significant differences between two parts. Freehand sections showed anthocyanin...
In recent years, transgenic technology has become the most important tool for molecular breeding. An efficient genetic transformation system is key to improving efficiency of biological breeding, and <italic>Agrobacterium</italic>-mediated common method used in plant experiments. Pineapple an tropical horticultural plant, but it a very narrow base, high genome heterozygosity, strict self-incompatibility, thus limiting value conventional breeding techniques. To shorten cycle create new...
Two somatic embryogenesis receptor-like kinase genes (identified as AcSERK1 and AcSERK2) have previously been characterized from pineapple (Ananas comosus). In this work, we describe the characterization of a third gene (AcSERK3) in family. AcSERK3 had all characteristic domains shared extensive sequence homology with other plant SERKs. expression was studied by situ hybridization quantitative real-time PCR to analyze its function. Intense signals were observed only single competent cells...
The R2R3-MYB proteins comprise the largest class of MYB transcription factors, which play an essential role in regulating anthocyanin synthesis various plant species. Ananas comosus var. bracteatus is important colorful anthocyanins-rich garden plant. spatio-temporal accumulation anthocyanins chimeric leaves, bracts, flowers, and peels makes it with a long ornamental period highly improves its commercial value. We conducted comprehensive bioinformatic analysis gene family based on genome...
Albino seedlings that arise during seed reproduction can have a significant impact on plant growth and breeding. In this research, we present the first report of albino occurrences in process Prunus salicina describe cytological, physiological, transcriptomic changes observed seedlings. The which were several plum cultivars exhibited abnormal chloroplast ultrastructure perturbed stomatal structure. Compared to normal seedlings, photosynthetic pigment contents decreased by more than 90%,...
Pineapple is one of the most economically important tropical or subtropical fruit trees. However, few studies focus on development its unique collective fruit. In this study, we generated a genome-wide developmental transcriptomic profile 14 different tissues pineapple covering each three major stages. total, 273 tissue-specific and 1,051 constitutively expressed genes were detected. We also performed gene co-expression analysis 18 modules classified. Among these, found interesting modules;...