A5075547375- Ion Channels and Receptors
- AI in cancer detection
- Radiomics and Machine Learning in Medical Imaging
- Ion channel regulation and function
- Connexins and lens biology
- Aquaculture disease management and microbiota
- Enzyme function and inhibition
- Cell death mechanisms and regulation
- Congenital Anomalies and Fetal Surgery
- Electrospun Nanofibers in Biomedical Applications
- Chemical Reactions and Isotopes
- RNA regulation and disease
- Planarian Biology and Electrostimulation
- Genomics, phytochemicals, and oxidative stress
- Ubiquitin and proteasome pathways
- Bacterial biofilms and quorum sensing
- Medical research and treatments
- Cell Image Analysis Techniques
- Phytochemicals and Antioxidant Activities
- Pediatric Hepatobiliary Diseases and Treatments
- Organ Transplantation Techniques and Outcomes
- Bioactive Compounds and Antitumor Agents
- Higher Education Teaching and Evaluation
- Free Radicals and Antioxidants
- Mitochondrial Function and Pathology
University of Valparaíso
2016-2024
Hospital Carlos Van Buren
2024
Temple University
1962
Rockefeller University
1961
With advancements in stem cell technology, vitro models using iPSC (induced pluripotent cells)-derived cardiomyocytes (iPSC-CM) and engineered heart tissues (EHT) can serve as powerful tools for disease modeling drug screening. ...Fluorescent reporters of cardiac electrophysiology provide valuable information on tissue function. However, motion artifacts caused by muscle contraction interfere with accurate measurement fluorescence signals. Although drugs ...
The transient receptor potential (TRP) ion channel family consists of a broad variety non-selective cation channels that integrate environmental physicochemical signals for dynamic homeostatic control. Involved in cellular physiological processes, TRP are fundamental to the control cell life cycle. from vanilloid (TRPV) have been directly implicated death. TRPV1 is activated by pain-inducing stimuli, including inflammatory endovanilloids and pungent exovanilloids, such as capsaicin (CAP)....
Background: Breast cancer is a malignant disease that represents an important public health burden. The description of new molecular markers can be to diagnosis, classification, and treatment. Transient receptor potential vanilloid 1 (TRPV1) polymodal channel expressed in different neoplastic tissues cell lines breast associated with the regulation tumor growth, neurogenesis, pain, progression cancer. In primary metastatic tumors, TRPV1 during transformation, invasive behavior, resistance...
Synaptic loss induced by soluble oligomeric forms of the amyloid β peptide (sAβos) is one earliest events in Alzheimer´s disease (AD) and thought to be major cause cognitive deficits. These abnormalities rely on defects synaptic plasticity, a series manifested as activity-dependent modifications structure function. It has been reported that Pannexin1 (Panx1), non-selective channel implicated cell communication intracellular signaling, modulates induction excitatory plasticity under...
The immunohistochemical (IHC) evaluation of epidermal growth factor 2 (HER2) for the diagnosis breast cancer is still qualitative with a high degree inter-observer variability, and thus requires incorporation complementary techniques such as fluorescent in situ hybridization (FISH) to resolve diagnosis. Implementing automatic algorithms classify IHC biomarkers crucial typifying tumor deciding on therapy each patient better performance. present study aims demonstrate that, using an...
17β-estradiol is a neuronal survival factor against oxidative stress that triggers its protective effect even in the absence of classical estrogen receptors. The polymodal transient receptor potential vanilloid subtype 1 (TRPV1) channel has been proposed as steroid implied tissue protection damage. We show here TRPV1 sufficient condition for to enhance metabolic performance injured cells. Specifically, expressing cells, application within first three hours avoided H2O2-dependent...
Biliary atresia precedes liver cirrhosis and transplantation. Amniotic membrane (AM) promotes tissue regeneration, inhibits fibrosis, reduces inflammation. Here, we test amniotic potential as a therapeutic tool against cholestatic fibrosis. Three groups of rats were used: sham surgery (SS), bile duct ligature (BDL), plus human (BDL + AM). After surgery, animals sacrificed at different weeks. Biochemical histopathological analyses performed. Collagen was expressed percentage total area. qPCR...
Abstract Vibrio ordalii is an extracellular, Gram‐negative bacterium that produces vibriosis in salmonids. While pathogenesis not fully understood, this has numerous likely genes for adhesion, colonization, invasion factors and, as recently suggested, intracellular behaviour. Therefore, study aimed to clarify possible behaviour V. Vo‐LM‐18 and ATCC 33509 T the fish‐cell lines SHK‐1 CHSE‐214. Confocal microscopy revealed inside cytoplasm both at 4 hr post‐inoculation (hpi). At 8 16 hpi,...
The quality assessment of biomaterials in pathological anatomy is crucial for the optimal diagnosis and treatment conditions like cancer. This exemplified immunohistochemistry profiling human epidermal growth factor receptor 2 (HER2) breast Therefore, it relevant to understand how preanalytical processes, such as post-surgery handling fixation quality, impact biomaterial diagnostic accuracy. study investigates first influence steps on performance HER2 diagnosis. Then a quantitative automated...
Cervical cancer (CC) remains a significant health issue, especially in low- and middle-income countries (LMICs). While Pap smears are the standard screening method, they have limitations like low sensitivity subjective interpretation. Liquid-based cytology (LBC) offers improvements but still relies on manual analysis. This study explored potential of deep learning (DL) for automated cervical cell classification using both LBC samples. A novel image segmentation algorithm was employed to...
Cervical cancer (CC) remains a significant health issue, especially in low- and middle-income countries (LMICs). While Pap smears are the standard screening method, they have limitations, like low sensitivity subjective interpretation. Liquid-based cytology (LBC) offers improvements but still relies on manual analysis. This study explored potential of deep learning (DL) for automated cervical cell classification using both LBC samples. A novel image segmentation algorithm was employed to...
Abstract Optogenetics is a molecular biological technique involving transfection of cells with photosensitive proteins and the subsequent study their effects. The aim this was to evaluate effect blue light on survival HeLa cells, transfected channelrhodopsin‐2 (ChR2). wild‐type were plasmid that contained gene for ChR2. Transfection channel function evaluated by real‐time polymerase chain reaction (RT‐PCR), fluorescence imaging using green fluorescent protein (GFP) flow cytometry...