Background Previous studies have shown that patients with systemic lupus erythematosus (SLE) tend to a higher risk of cardiovascular disease (CVD), but the potential causal relationship between genetic susceptibility SLE and CVD is not clear. This study systematically investigated association genetically determined CVD. Methods The tools were obtained from genome-wide CVD, no overlap their participating populations. Mendelian randomization (MR) analysis was performed using inverse variance...

Background A large number of observational studies showed that patients with psoriasis have a higher risk cardiovascular disease (CVD), but most did not fully adjust for confounding factors, so it is clear whether the CVD directly attributed to psoriasis. We used Mendelian randomization (MR) evaluate potential causal relationship between and CVD. Methods genetic instruments from genome-wide association study (GWAS) European descent investigate its Inverse variance-weighted (IVW) MR analyses...

<title>Abstract</title> Background Fibrobalst plays a critical role in heart physiology and pathology, yet their immunological transplanted grafts remains unclear, particularly the mechanisms involved. Methods Heart transplantation was performed mouse models (C57BL/6J, BALB/c, Postn^MCM, Rosa26-tdTomato, ATG5^floxp/floxp). Single-cell RNA sequencing, qPCR, western blotting, flow cytometry, immunofluorescence staining were used to identify fibroblast subpopulations assess...

Renal function is a crucial factor affecting the prognosis of patients with end-stage heart failure (HF). The differential impacts transplantation and left ventricular assist device (LVAD) implantation on renal are unclear. Therefore, we compared perioperative changes in who underwent that LVAD implantation. This study included 77 59 at five hospitals between January 2019 December 2023. These were divided into two groups based surgery type: or estimated glomerular filtration rates (eGFRs)...

Polyphyllin I (PPI), a small molecular monomer extracted from Rhizoma of Paris polyphyllin, shows strong anticancer effects in previous study. Human lung adenocarcinoma A549 cells, human squamous cell carcinoma SK-MES-1 and large H460 cells were cultured then treated with PPI. Cell proliferation apoptosis measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, flow cytometry, western blot analysis, DNA ladder. Athymic nude mice bearing tumors injected PPI, tumor...

MicroRNAs (miRNAs) are one class of non-coding RNAs that play an important role in post-transcriptional regulation via the degradation or translational inhibition their target genes. MicroRNA-150 (miR-150) plays a vital regulating development B and T lymphocytes. Although dysregulation miR-150 was confirmed human myocardial infarction, little is known regarding biological functions response to reactive oxygen species (ROS)-mediated gene cardiac myocytes. Using quantitative real-time reverse...

Background Although studies suggest that tea consumption is associated with a reduced risk of cardiovascular disease (CVD). There no unified conclusion about the potential relationship between drinking and CVD. We used two-sample Mendelian randomized (MR) analysis to systematically explore causal intake CVD subtypes for first time. Furthermore mediating effect hypertension was also explored by two-step MR. Methods Genetic instruments were identified from genome-wide association (GWAS)...

Background: Taurine upregulated gene 1 (TUG1) has been identified on long noncoding RNA (lncRNA); however, its function in myocardial cells following ischemia/ reperfusion (I/R) injury not explored. This study aimed to investigate the role of LncTUG1 I/R by focusing relationship with autophagy induction regulating miR-34a-5p expression. Methods: We established a model and H9C2 hypoxia-ischemic reoxygenation (HI/R) conditions induce injury. TTC, Western blot, CCK-8 assay, quantitative reverse...

Background. While observation studies have shown a positive correlation between inflammatory bowel disease (IBD) and the risk of nonmalignant digestive system diseases, definitive causal relationship has not yet been clearly established. Methods. Mendelian randomization (MR) was employed to investigate potential association genetic susceptibility IBD gastrointestinal diseases. Genetic variants were extracted as instrumental variables (IVs) from genome-wide study (GWAS) meta-analysis, which...

Background Although studies suggest that concentrations of serum 25-hydroxyvitamin D (25(OH)D) are lower in individuals with Heart Failure (HF), the beneficial effects vitamin supplementation controversial. Therefore, this study, we aimed to determine whether there is a causal relationship between Vitamin (VD) levels and HF. Methods We obtained genetic instruments from largest available genome-wide association study (GWAS) European descent for 25(OH)D (443, 734 individuals) investigate HF...

Type I insulin-like growth factor receptor (IGF-IR), which is frequently overexpressed in a variety of human cancers including lung cancer, mediates cancer cell proliferation and tumor growth. In this study, we used U6 promoter-driven DNA-template approach to induce hairpin RNA (hpRNA)-triggered RNAi silence IGF-IR gene expression the line A549, then evaluate its effects on apoptosis, apoptosis-related expression, cells vitro nude mice. levels were found markedly decrease transfected with...

The cardioprotective effect of FSTL1 has been extensively studied in recent years, but its role myocardial ischemia/reperfusion injury (IRI) is unclear. In this study, we investigated the pretreatment on IRI as well possible involvement autophagic pathways effects.The effects viability and apoptosis rat cardiomyocytes were after exposure to hypoxia/ischemia by using CCK-8 assay Annexin V/PI staining. Further, western blot analysis was used detect autophagy-associated proteins, confocal...

The role of growth differentiation factor (GDF)-11 in cardiac diseases has not been fully determined. Our study revealed that GDF-11 is essential for myocardial development and physiological growth, whereas its absence exacerbates heart failure under pressure overload condition via impairing the responsive angiogenesis. induced VEGF expression CMs by activating Akt/mTOR pathway. effect endogenous on belongs to local self-regulation tissue, rather than a way systemic regulation.

Abstract Deep hypothermic circulatory arrest (DHCA) can cause acute lung injury (ALI), and its pathogenesis mimics ischaemia/reperfusion (I/R) injury. Autophagy is also involved in I/R The present study aimed to elucidate whether DHCA induces natural autophagy activation role DHCA‐mediated Here, rats were randomly assigned the Sham or group. sham group ( n = 5) only received anaesthesia air intubation. underwent cardiopulmonary bypass (CPB) followed by procedure. then sacrificed at 3, 6 24 h...

Abstract The type 1 insulin-like growth factor receptor (IGF-1R), which is over-expressed or activated in many human cancers, including lung cancer, mediates cancer cell proliferation and metastasis. Several studies indicate that blocking IGF-1R expression can inhibit tumor In this study, inhibition of the endogenous by recombinant adenoviruses encoding short hairpin RNAs against was found to significantly suppress expression, arrest cycle, enhance apoptotic response, proliferation,...

The effects of RNA interference-mediated insulin-like growth factor 1 receptor (IGF1R) gene silencing in response to cisplatin (DDP) the lung cancer cell line A549 vivo and vitro were investigated using two plasmids expressing short hairpin (shRNA) IGF1R. cells transfected with each shRNA then treated DDP. Semi-quantitative reverse transcription-PCR Western blot analysis used detect expression MTT assay, flow cytometry tumor assay athymic nude mice assess chemosensitivity DDP following IGF1R...

Cancer invasion and metastasis, involving a variety of pathological processes cytophysiological changes, contribute to the high mortality lung cancer. The type 1 insulin-like growth factor receptor (IGF-1R), associated with cancer progression invasion, is potential anti-invasion anti-metastasis target in To inhibit invasive properties cells, we successfully down-regulated IGF-1R gene expression A549 human cells by small interfering RNA (siRNA) technology, evaluated its effects on...

Background: We aimed to analyze the short-term clinical outcomes of transapical aortic valve replacement (TA-TAVR) compared with surgical (SAVR) in symptomatic regurgitation (AR) patients draw preliminary conclusions about advantages and disadvantages TA-TAVR SAVR provide evidence for future use AR patients. Method: From September 2016 2021, 69 undergoing J-valve implantation 42 at Second Hospital Zhejiang University School Medicine were analyzed data 30-day follow-up compare differences...