Selenium deficiency has been proven to induce calcium disorders in the chicken heart. However, detailed regulatory mechanisms, e.g., long noncoding RNA (lncRNA)-microRNA (miRNA)-mRNA axis, have not yet described. Here, we point out lnc-2315, miR-1594, and Troponin T (TNNT2) based on results of lncRNA miRNA comparative genomics group analysis Se-deficient hearts compared with control hearts. We employed lnc-3215 TNNT2 knockdown, miR-1594 overexpression models embryos vivo, lnc-3215, knockdown...

Long non-coding RNAs (lncRNAs) as a key group of have gained widely attention. Though lncRNAs been functionally annotated and systematic explored in higher mammals, few are under systematical identification annotation. Owing to the expression specificity, known expressed embryonic brain tissues remain still limited. Considering large number only transcribed tissues, studies developmental therefore special interest. Here, publicly available RNA-sequencing (RNA-seq) data integrated identify...

Gastric cancer remains an incurable malignance and the second leading cause of death globally. Recent progress in gastric research has demonstrated crucial roles stem cells (CSCs) development, metastasis, drug resistance this disease. Various studies have highlighted role long noncoding RNAs (lncRNAs) pathogenesis cancer. In study, through fluorescence-activated cell sorting, we isolated CSCs (GCSCs) from MKN-45 for first time that lncRNA ROR was highly expressed CD133+ GCSCs. Overexpression...

Exercise is beneficial for muscle atrophy. Peroxisome proliferator-activated receptor gamma (PPARγ) and microRNA-29b (miR-29b) have been reported to be responsible angiotensinⅡ (AngⅡ)-induced However, it unclear whether exercise can protect AngⅡ-induced atrophy by targeting PPARγ/miR-29b. Skeletal in both the control group run was established AngⅡ infusion; after 1 week of training, mice were sacrificed, weight determined. Myofiber size measured hematoxylin-eosin wheat-germ agglutinin...

Abstract Although sex determination and differentiation are key developmental processes in animals, the involvement of non-coding RNA regulation this process is still not clarified. The tiger pufferfish ( Takifugu rubripes ) one most economically important marine cultured species Asia, but analyses miRNA long (lncRNA) at early stages have been conducted yet. In our study, high-throughput sequencing technology was used to sequence transcriptome libraries from undifferentiated gonads T. ....

Disturbances in DNA damage repair may lead to cancer. SIRT1, an NAD+-dependent deacetylase, plays a crucial role maintaining cellular homeostasis through the regulation of processes such as histone posttranslational modifications, repair, and metabolism. However, comprehensive exploration SIRT1's involvement pan-cancer remains lacking. Our study aimed analyze SIRT1 gain more understanding its multiple malignancies. We systematically examined by analyzing data from The Cancer Genome Atlas...

To compare the clinical outcomes of different systemic therapies, specifically PD(L)1 inhibitors plus Lenvatinib versus Atezolizumab Bevacizumab, when combined with hepatic arterial infusion chemotherapy (HAIC) based on FOLFOX regimen (oxaliplatin, fluorouracil, and leucovorin) as first line treatment for unresectable hepatocellular carcinoma. This real-world retrospective study enrolled 294 patients HCC. All received HAIC in combination either (PLEN-HAIC) or Bevacizumab (AT-HAIC)....

Abstract Aims Cardiovascular diseases, such as atrial fibrillation, coronary artery disease, heart failure (HF) and ischaemic stroke, are leading causes of death globally exert major global health burden. Recent studies suggest that glucagon‐like peptide‐1 receptor agonists (GLP1Ra), a novel class antidiabetic drugs, may not only help manage blood glucose but also reduce the risks cardiovascular diseases. However, mechanisms through which GLP1Ra protects against remain incompletely...

Abstract Promoting the paracrine effects of human mesenchymal stem cell (hMSC) therapy may contribute to improvements in patient outcomes. Here we develop an innovative strategy enhance hMSCs. In a mouse hindlimb ischaemia model, examine hMSCs which novel triple-catalytic enzyme is introduced stably produce prostacyclin (PGI 2 -hMSCs). We show that PGI -hMSCs facilitate perfusion recovery and running capability as compared with control or iloprost (a stable analogue). Transplanted do not...

Colorectal cancer (CRC) is one of the most lethal malignances in humans. Hence, it great significance to identify regulatory molecules CRC progression. Accumulating evidence has demonstrated that long non‑coding RNAs (lncRNAs) are involved malignancy. It been reported intergenic non‑protein coding RNA 857 (LINC00857) acts as a vital oncogene many types by promoting cell proliferation and migration. However, role LINC00857 remains unclear. In present study, was upregulated tissue samples...

Muscle atrophy is debilitating and can be induced by several stressors. Unfortunately, there are no effective pharmacological treatment until now. MicroRNA (miR)-29b an important target that we identified to commonly involved in multiple types of muscle atrophy. Although sequence-specific inhibition miR-29b has been developed, this study, report a novel small-molecule inhibitor targets hairpin precursor (pre-miR-29b) (Targapremir-29b-066 [TGP-29b-066]) considering both its three-dimensional...

Sevoflurane, a commonly used volatile anesthetic, recently has been found neurotoxicity in the central nervous system of neonatal rodents. This study aimed to reveal whether phosphodiesterase 4 (PDE-4) inhibitor roflumilast protective functions sevoflurane-induced nerve damage.Hippocampal neurons were isolated from juvenile rats, and exposed sevoflurane with or without treatment. Cell viability apoptosis respectively assessed by CCK-8 flow cytometry. Western blot analysis was performed...

Purpose: Acute ischemic stroke (AIS) is a leading health problem caused by cerebral ischemia/reperfusion (CI/R). This study aimed to unveil the potential clinical value and mechanism of lncRNA CASC15. Patients Methods: The expression CASC15, miR-338-3p was detected quantitative real-time PCR (qRT-PCR). correlations between CASC15 national institutes scale (NIHSS) scores or were evaluated Pearson correlation. A receiver operating characteristic (ROC) curve performed provide diagnostic Cell...

Abstract Background Icariin, a traditional Chinese medicine, has demonstrated anti-osteoporotic properties in ovariectomized mice. However, its effectiveness preventing bone loss induced by ketogenic diet (KD), which mimics osteoporosis human, remains unexplored. This study aims to investigate icariin’s impact on KD-induced Methods Thirty mice were divided into: sham, KD, and KD + icariin groups. Post 12-week intervention, evaluation including microstructures, serum concentrations of...

Growth hormone-releasing hormone (GHRH) agonist, a 29-amino acid peptide, shows significant potential in treating myocardial infarction (MI) by aiding the repair of injured heart tissue. The challenge lies effective on-site delivery GHRH agonist. This study explores use targetable system employing ROS-responsive PEG-PPS-PEG polymers to encapsulate and deliver agonist MR409 for enhanced therapeutic efficacy.

Abstract Age‐related diseases such as cardiovascular portend disability, increase health expenditures, and cause late‐life mortality. Synthetic agonists of growth hormone‐releasing hormone (GHRH) exhibit several favorable effects on heart function remodeling. Here we assessed whether GHRH agonist MR409 can modulate systemic parameters in old mice. Starting at the age 15 months, mice were injected subcutaneously with (10 µg/day, n = 8) or vehicle ( 7) daily for 6 months. Mice treated showed...

The role of growth differentiation factor (GDF)-11 in cardiac diseases has not been fully determined. Our study revealed that GDF-11 is essential for myocardial development and physiological growth, whereas its absence exacerbates heart failure under pressure overload condition via impairing the responsive angiogenesis. induced VEGF expression CMs by activating Akt/mTOR pathway. effect endogenous on belongs to local self-regulation tissue, rather than a way systemic regulation.

Allogeneic hematopoietic stem cell transplantation (aHSCT) is utilized as a potential curative treatment for various hematologic malignancies. However, graft-versus-host disease (GVHD) post-aHSCT severe complication that significantly impacts patients' quality of life and overall survival, becoming major cause non-relapse mortality. In recent years, the association between epigenetics GVHD has garnered increasing attention. Epigenetics focuses on studying mechanisms affect gene expression...

Abstract RE (Radiation enteritis) has been characterized by the inflammation reaction, and in this study, we aim to explore inflammatory cytokines underlying mechanism involved pathogenesis of RE. Luciferase assay was performed whether polymorphism affected expression let‐7, also validated let‐7 directly regulated f IL‐6 expression. Then Elisa study rs13293512 associated with enteritis occurrence. And Western‐blot real‐time PCR were verify relationship between IL‐6. 380 colorectal cancer...