A5077877591- Genomics and Phylogenetic Studies
- Acute Myeloid Leukemia Research
- Plant Virus Research Studies
- RNA modifications and cancer
- Insect-Plant Interactions and Control
- Cancer Genomics and Diagnostics
- Plant Pathogens and Resistance
- Tissue Engineering and Regenerative Medicine
- Bacterial Infections and Vaccines
- Insect symbiosis and bacterial influences
- Acute Lymphoblastic Leukemia research
- Plant Pathogens and Fungal Diseases
- Renal Diseases and Glomerulopathies
- Immune cells in cancer
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Plant and Fungal Interactions Research
- Genomics and Rare Diseases
- Neonatal Respiratory Health Research
- Hematopoietic Stem Cell Transplantation
- Plant Pathogenic Bacteria Studies
- Neuroblastoma Research and Treatments
- Agronomic Practices and Intercropping Systems
- COVID-19 Clinical Research Studies
- Chronic Kidney Disease and Diabetes
Nationwide Children's Hospital
2020-2024
The Ohio State University
2013-2023
Imaging Center
2012-2023
Rasmussen College
2023
Center for Genomic Science
2022
Sejong University
2015
Agricultural Research Service
2014
College of Wooster
2014
Université d'Avignon et des Pays de Vaucluse
2014
Quality Research
2014
The concurrent development of high-throughput genotyping platforms and next generation sequencing (NGS) has increased the number density genetic markers, efficiency constructing detailed linkage maps, our ability to overlay recombination physical maps genome. We developed an array for tomato with 8,784 Single Nucleotide Polymorphisms (SNPs) mainly discovered based on NGS-derived transcriptome sequences. Of SNPs, 7,720 (88%) passed manufacturing quality control could be scored in germplasm....
Abstract Background Bed bugs ( Cimex lectularius ) are hematophagous nocturnal parasites of humans that have attained high impact status due to their worldwide resurgence. The sudden and rampant resurgence C. has been attributed numerous factors including frequent international travel, narrower pest management practices, insecticide resistance. Results We performed a next-generation RNA sequencing (RNA- Seq experiment find differentially expressed genes between pesticide-resistant (PR)...
The digestive and respiratory tracts of chickens are colonized by bacteria that believed to play important roles in the overall health performance birds. Most current research on commensal (microbiota) has focused broilers gut microbiota, less attention been given layers microbiota. This bias left significant gaps our knowledge layer microbiome. study was conducted define core microbiota colonizing upper tract (URT) lower intestinal (LIT) commercial under field conditions. One hundred...
Maize lethal necrosis (MLN), a severe virus disease of maize, has emerged in East Africa recent years with devastating effects on production and food security where maize is staple subsistence crop. In extensive surveys MLN-symptomatic plants Africa, sequences Johnsongrass mosaic (JGMV) were identified Uganda, Kenya, Rwanda, Tanzania. The African JGMV distinct from previously reported isolates infects sorghum, but not wheat or oat. This isolate causes MLN coinfection chlorotic mottle (MCMV),...
Phosphatase and tensin homologue (PTEN) regulates cell growth survival through inhibition of the mammalian target rapamycin (MTOR) signalling pathway. Germline genetic variation PTEN is associated with autism, macrocephaly hamartoma tumour syndromes. The effect developmental somatic mutations on nervous system phenotypes not well understood, although brain mosaicism MTOR pathway genes an emerging cause cortical dysplasia epilepsy in paediatric population. Here we report two variants...
Abstract Background Cancers exhibit complex transcriptomes with aberrant splicing that induces isoform-level differential expression compared to non-diseased tissues. Transcriptomic profiling using short-read sequencing has utility in providing a cost-effective approach for evaluating isoform expression, although assembly displays limitations the accurate inference of full-length transcripts. Long-read RNA (Iso-Seq), Pacific Biosciences (PacBio) platform, can overcome such by sequence...
Single exon duplications account for disease in a minority of Duchenne muscular dystrophy patients. Exon skipping these patients has the potential to be highly therapeutic through restoration full-length dystrophin expression. We conducted 48-week open label study casimersen and golodirsen 3 subjects with an 45 or 53 duplication. Two (aged 18 23 years) were non-ambulatory at baseline. Upper limb, pulmonary, cardiac function appeared stable 2 whom they could evaluated. Dystrophin expression...
Insects rely on olfaction to locate food, mates, and suitable oviposition sites for successful completion of their life cycle. Agrilus planipennis Fairmaire (emerald ash borer) is a serious invasive insect pest that has killed tens millions North American (Fraxinus spp) trees threatens the very existence genus Fraxinus. Adult A. are attracted host volatiles conspecifics; however, date no molecular knowledge exists in planipennis. Hence, we undertook an antennae-specific transcriptomic study...
Pollination reduces flower longevity in many angiosperms by accelerating corolla senescence. This response requires hormone signaling between the floral organs and results degradation of macromolecules organelles within petals to allow for nutrient remobilization developing seeds. To investigate early pollination-induced changes petal gene expression, we utilized high-throughput sequencing identify transcripts that were differentially expressed corollas pollinated Petunia × hybrida flowers...
Insects are the most important epidemiological factors for plant virus disease spread, with >75% of viruses being dependent on insects transmission to new hosts. The black-faced leafhopper (Graminella nigrifrons Forbes) transmits two that use different strategies transmission: Maize chlorotic dwarf (MCDV) which is semi-persistently transmitted and fine streak (MFSV) persistently propagatively transmitted. To date, little known regarding molecular cellular mechanisms in regulate process...
The two major U.S. maize viruses, Maize dwarf mosaic virus (MDMV) and chlorotic (MCDV), emerged in southern Ohio surrounding regions the 1960s caused significant losses. Planting resistant varieties changing cultural practices has dramatically reduced impact subsequent decades. Current information on distribution, diversity, of known potential disease-causing viruses is lacking. To assess current reservoir present at sites past disease emergence, we used a combination serological testing...
Survival of patients with acute myeloid leukemia (AML) is inversely associated age, but the impact race on outcomes adolescent and young adult (AYA; range, 18-39 years) unknown. We compared survival 89 non-Hispanic Black 566 White AYA AML treated frontline Cancer Leukemia Group B/Alliance for Clinical Trials in Oncology protocols. Samples 327 (50 277 White) were analyzed via targeted sequencing. Integrated genomic profiling was performed select longitudinal samples. had worse outcomes,...
Congenital heart disease (CHD) is a common group of birth defects with strong genetic contribution to their etiology, but historically the diagnostic yield from exome studies isolated CHD has been low. Pleiotropy, variable expressivity, and difficulty accurately phenotyping newborns contribute this problem. We hypothesized that performing sequencing on selected individuals in families multiple members affected by left-sided CHD, then filtering variants population frequency, silico predictive...
Abstract Background Macrophages play important roles in phagocytosing tumor cells. However, tumors escape macrophage phagocytosis part through the expression of anti-phagocytic signals, most commonly CD47. In Ewing sarcoma (ES), we found that cells utilize dual mechanisms to evade clearance by simultaneously over-expressing CD47 and down-regulating cell surface calreticulin (csCRT), pro-phagocytic signal. Here, investigate combination a blockade (magrolimab, MAG) inhibit signal chemotherapy...
Phytophthora sojae is the primary pathogen of soybeans that are grown on poorly drained soils. Race-specific resistance to P. in soybean gene-for-gene, although many areas US and worldwide there populations have adapted most commonly deployed ( Rps) genes. Hence, this system has received increased attention towards identifying mechanisms molecular markers associated with partial pathogen. Several quantitative trait loci (QTL) been identified cultivar 'Conrad' contributes expression multiple...
Bacterial spot of tomato is caused by at least four species Xanthomonas with multiple physiological races. We developed a complex breeding population for simultaneous discovery marker-trait linkage, validation existing quantitative trait loci (QTL), and pyramiding resistance. Six advanced accessions resistance from distinct sources were crossed in all combinations their F1 hybrids intercrossed. Over 1,100 segregating progeny evaluated the field following inoculation X. euvesicatoria race T1...
Pediatric cancers typically have a distinct genomic landscape when compared to adult and frequently carry somatic gene fusion events that alter expression drive tumorigenesis. Sensitive specific detection of fusions through the analysis next-generation-based RNA sequencing (RNA-Seq) data is computationally challenging may be confounded by low tumor cellularity or underlying complexity. Furthermore, numerous computational tools are available identify from supporting RNA-Seq reads, yet each...
Evidence suggests that COVID-19 predisposes to cardiovascular diseases (CVDs). While monocytes/macrophages play a central role in the immunopathogenesis of atherosclerosis, less is known about their immunopathogenic mechanisms lead CVDs during COVID-19. Natural killer (NK) cells, which an intermediary pathologies like are dysregulated Here, we sought investigate altered immune cells and associations with CVD risk severe
Dodders are among the most important parasitic plants that cause serious yield losses in crop plants. In this report, we sought to unveil genetic basis of dodder parasitism by profiling trancriptomes Cuscuta pentagona and C. suaveolens, two common species using a next-generation RNA sequencing platform. De novo assembly sequence reads resulted more than 46,000 isotigs contigs (collectively referred as expressed tags or ESTs) for each species, with half them predicted encode proteins share...
T cells redirected to cancer either via a chimeric antigen receptor (CAR-T) or bispecific molecule have been breakthrough technologies; however, CAR-T require individualized manufacturing and bispecifics generally continuous infusions. We created an off-the-shelf, single-dose solution for achieving prolonged systemic serum levels of protein immunotherapeutics adeno-associated virus (AAV) gene transfer. demonstrate proof principle in CD19 + lymphoma xenograft model using single intravenous...
Abstract Chronic lung disease has been attributed to stem cell aging and/or exhaustion. We investigated these mechanisms using mouse and human tracheobronchial tissue-specific cells (TSC). In mouse, chromatin labeling flow cytometry demonstrated that naphthalene (NA) injury activated a subset of TSC. These TSC continued proliferate after the epithelium was repaired clone study ~96% underwent terminal differentiation. Despite attrition, epithelial repair second NA normal. The accelerated...