A5015732671- Sleep and related disorders
- Sleep and Wakefulness Research
- Cancer Genomics and Diagnostics
- Sleep and Work-Related Fatigue
- Glioma Diagnosis and Treatment
- CAR-T cell therapy research
- Virus-based gene therapy research
- RNA modifications and cancer
- Posttraumatic Stress Disorder Research
- Genomics and Rare Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Acute Myeloid Leukemia Research
- Immune cells in cancer
- School Health and Nursing Education
- Immunotherapy and Immune Responses
- Chromatin Remodeling and Cancer
- Genomic variations and chromosomal abnormalities
- Childhood Cancer Survivors' Quality of Life
- Neuroblastoma Research and Treatments
- Single-cell and spatial transcriptomics
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- BRCA gene mutations in cancer
- Alzheimer's disease research and treatments
- Immune Cell Function and Interaction
Minneapolis VA Health Care System
2017-2025
Nationwide Children's Hospital
2017-2025
Twin Cities Orthopedics
2025
University of Minnesota
2024-2025
Pediatrics and Genetics
2024
The Ohio State University
2017-2024
Rasmussen College
2020-2024
Philadelphia VA Medical Center
2019-2023
Veterans Health Administration
2019-2023
University of Pennsylvania
2019-2023
Recent studies have shown the importance of dynamic tumor microenvironment (TME) in high-grade gliomas (HGGs). In particular, myeloid cells are known to mediate immunosuppression glioma; however, it is still unclear if play a role low-grade glioma (LGG) malignant progression. Here, we investigate cellular heterogeneity TME using single-cell RNA sequencing murine model that recapitulates progression LGG HGG. LGGs show increased infiltrating CD4
Abstract Objective Epilepsy‐associated developmental lesions, including malformations of cortical development and low‐grade tumors, represent a major cause drug‐resistant seizures requiring surgical intervention in children. Brain‐restricted somatic mosaicism has been implicated the genetic etiology these lesions; however, many contributory genes remain unidentified. Methods We enrolled 50 children who were undergoing epilepsy surgery into translational research study. Resected tissue was...
Abstract Despite some success in secondary brain metastases, targeted or immune-based therapies have shown limited efficacy against primary malignancies such as glioblastoma (GBM). Although the intratumoral heterogeneity of GBM is implicated treatment resistance, it remains unclear whether this diversity observed within metastases and to what extent cancer cell–intrinsic sculpts local immune microenvironment. Here, we profiled immunogenomic state 93 spatially distinct regions from 30...
Background: Familial non-medullary thyroid cancer (NMTC) accounts for a relatively small proportion of cases, but it displays strong genetic predisposition. So far, only few NMTC susceptible genes and low-penetrance variants contributing to have been described. This study aimed identify rare germline that may predispose individuals by sequencing cohort 17 families. Methods: Whole-genome genome-wide linkage analysis were performed in MendelScan BasePlayer applied screen followed customized...
Phosphatase and tensin homologue (PTEN) regulates cell growth survival through inhibition of the mammalian target rapamycin (MTOR) signalling pathway. Germline genetic variation PTEN is associated with autism, macrocephaly hamartoma tumour syndromes. The effect developmental somatic mutations on nervous system phenotypes not well understood, although brain mosaicism MTOR pathway genes an emerging cause cortical dysplasia epilepsy in paediatric population. Here we report two variants...
Abstract Variants in the AUTS2 gene are associated with a broad spectrum of neurological conditions characterized by intellectual disability, microcephaly, and congenital brain malformations. Here, we use human cerebral organoid model to investigate pathophysiology heterozygous de novo missense variant identified patient multiple impairments including primary microcephaly profound disability. Proband organoids exhibit reduced growth, deficits neural progenitor cell (NPC) proliferation...
Previously, clinical trials of experimental virotherapy for recurrent glioblastoma multiforme (GBM) demonstrated that inoculation with a conditionally replication-competent Δγ134.5 oncolytic herpes simplex virus (oHSV), G207, was safe. Following the initial safety study, phase Ib trial enrolled 6 adult patients diagnosed GBM recurrence from which tumor tissue banked future studies.Here, we analyzed RNA sequencing (RNA-seq) data obtained pre- and posttreatment (collected 2 or 5 days after...
Abstract Background Cancers exhibit complex transcriptomes with aberrant splicing that induces isoform-level differential expression compared to non-diseased tissues. Transcriptomic profiling using short-read sequencing has utility in providing a cost-effective approach for evaluating isoform expression, although assembly displays limitations the accurate inference of full-length transcripts. Long-read RNA (Iso-Seq), Pacific Biosciences (PacBio) platform, can overcome such by sequence...
Understanding the pathways regulating normal and malignant hematopoietic stem cell (HSC) biology is important for improving outcomes patients with hematologic disorders. Epithelial Growth Factor Like-7 (EGFL7 ) ∼30 kDa secreted protein that highly expressed in adult HSCs. Using Egfl7 genetic knock-out ( KO) mice recombinant EGFL7 (rEGFL7) protein, we examined role of hematopoiesis. We found KO had decreases overall BM cellularity resulting significant reduction number progenitor cells...
Abstract Glioblastoma is a highly lethal brain cancer that known for extensive intratumoral genomic heterogeneity. This characteristic makes it difficult to comprehensively identify set of tumor-unique neoantigens suitable developing personalized vaccine each patient. Our collaborative work has helped refine this therapeutic approach, encompassing three FDA-IND clinical trials, with increasing refinement. In practice, we utilize multi-region sampling the resected GBM from patient gain an...
Insomnia affects up to 22% of the U.S. adult population. The use mobile health applications (mHealth apps) has been posited as one way increase access evidence-based interventions for insomnia, such cognitive behavioral therapy insomnia (CBT-I). purpose current study was summarize availability mHealth apps that focus on providing users with and/or skills manage assess their adherence principles, and examine usability. terms "insomnia," "insomnia treatment," "sleep treatment" were used search...
Many genetic studies of intractable epilepsy in pediatric patients primarily focus on inherited, constitutional deficiencies identified patient blood. Recently, have revealed somatic mosaicism associated with which variants are present only a subset brain cells. We hypothesize that tissue-specific, represents an important etiology and aim to discover alterations epilepsy-affected tissue.We pursued research study identify mosaicism, using next-generation sequencing (NGS) technologies,...
Sexual assault survivors are at risk for a number of mental and physical health problems, including posttraumatic stress disorder anxiety. Unfortunately, few seek or services after sexual (Price, Davidson, Ruggiero, Acierno, & Resnick, 2014). Mitigating the impact via early interventions is growing important area research. This study adds to this literature by replicating expanding previous studies (e.g., Amstadter, Self-Brown, Kilpatrick, 2007) examining efficacy brief video-based...
Objective: Clinicians' perceptions of CBT-I Coach, a patient-facing mobile app for cognitive-behavioral therapy insomnia (CBT-I), are critical to its adoption and integration into practice. Diffusion innovations theory emphasizes the influence perceptions, including relative advantage current practice, compatibility clinicians' needs, complexity, innovation's trialability, observability. This study intended evaluate use Coach among Veterans Affairs (VA)-trained clinicians. Participants...
Survival of patients with acute myeloid leukemia (AML) is inversely associated age, but the impact race on outcomes adolescent and young adult (AYA; range, 18-39 years) unknown. We compared survival 89 non-Hispanic Black 566 White AYA AML treated frontline Cancer Leukemia Group B/Alliance for Clinical Trials in Oncology protocols. Samples 327 (50 277 White) were analyzed via targeted sequencing. Integrated genomic profiling was performed select longitudinal samples. had worse outcomes,...
Idiopathic inflammatory myopathies (IIM) are a group of autoimmune diseases characterised by myositis-related autoantibodies plus infiltration leucocytes into muscles and/or the skin, leading to destruction blood vessels and muscle fibres, chronic weakness fatigue. While complement-mediated capillary endothelia is implicated in paediatric adult dermatomyositis, complex diversity complement
Nightmares and sleep disturbances are gaining attention as targets of treatment interventions for trauma-exposed populations. Measures in trials evaluating these tend to utilize proprietary measures nightmare characteristics, which makes it challenging compare findings across studies. The Trauma-Related Nightmare Survey is a questionnaire that was initially developed utilization efficacy studies Exposure, Relaxation, Rescripting Therapy. Preliminary examinations the psychometric properties...