A5101524391- Cytokine Signaling Pathways and Interactions
- Natural Compounds in Disease Treatment
- Cervical Cancer and HPV Research
- Autophagy in Disease and Therapy
- Herpesvirus Infections and Treatments
- Pharmacological Effects of Natural Compounds
- Hepatitis B Virus Studies
- Psoriasis: Treatment and Pathogenesis
- Respiratory viral infections research
- Nail Diseases and Treatments
- interferon and immune responses
- Influenza Virus Research Studies
- Immune Response and Inflammation
- Peroxisome Proliferator-Activated Receptors
- Toxin Mechanisms and Immunotoxins
- Plant Disease Resistance and Genetics
- Viral-associated cancers and disorders
- Quinazolinone synthesis and applications
- Plant Virus Research Studies
- Mosquito-borne diseases and control
- Phytochemical Studies and Bioactivities
- Immunotherapy and Immune Responses
- Fungal Infections and Studies
- Dermatology and Skin Diseases
- T-cell and Retrovirus Studies
Ministry of Agriculture and Rural Affairs
2024
China Agricultural University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2009-2021
Fourth Affiliated Hospital of China Medical University
2020
China Medical University
2020
Beijing Center for Disease Prevention and Control
2019
Institute of Dermatology
1999-2005
Academy of Medical Sciences
1999-2000
Abstract Microinfarcts, the “invisible lesions”, are prevalent in aged and injured brains associated with cognitive impairments, yet their neurophysiological impact remains largely unknown. Using a multimodal chronic neural platform that combines functional microvasculature imaging spatially resolved recording, neurovascular effect of single microinfarct is investigated. Unlike larger strokes, microinfarcts induced only temporary suppression activity minimal cell death, recovery paralleling...
Sorafenib has revolutionized treatment of hepatocellular carcinoma (HCC), but its efficacy is limited by drug resistance. Autophagy the process which cellular components are transported to lysosomes for degradation, promotes energy production and macromolecular precursors. Studies have suggested that cytoprotective function autophagy may contribute chemoresistance or targeted resistance in cancer cells. We investigated effects miR-375 autophagy-related protein 14, their interrelationships,...
Tinea capitis is still common in developing countries, such as China. Its pathogen spectrum varies across regions and changes over time.This study aimed to clarify the current epidemiological characteristics of tinea China.A multicentre, prospective descriptive involving 29 tertiary hospitals China was conducted. From August 2019 July 2020, 611 patients with were enrolled. Data concerning demography, risk factors fungal tests collected. When necessary, pathogens further identified by...
Interferon-gamma (IFN-γ) signaling in keratinocytes plays an important role IFN-γ-mediated skin inflammation involved psoriasis. Blocking IFN-γ signal transduction could be a strategy for controlling inflammatory disorders. Tripterygium wilfordii Hook. F. (T. wilfordii) has been used effectively psoriasis treatment China. Its therapeutic mechanism on IFN-γ-dependent not elucidated. Triptolide is one of main components T. wilfordii's antiinflammatory and immune effects. This study aimed to...
LncRNA HCG18 is considered to be an oncogene in many types of tumors. The aim this study was explore the role lncRNA gastric cancer (GC).HCG18 levels GC tissues were detected. Potential biological influences on cell phenotypes examined by Cell Counting Kit-8 (CCK-8), wound healing and transwell assay. Subsequently, bioinformatics analysis, Chromatin immunoprecipitation (ChIP), Luciferase assay rescue experiments conducted identify regulatory network GC.It found that upregulated samples,...
Condyloma acuminatum (CA) is one of the most common sexually transmitted diseases and induced by low-risk human papillomaviruses (HPVs), mainly HPV type 6 11. Here, we report identification (-)-Epigallocatechin-3-gallate (EGCG) an HPV11 infection cell model.The recombined HPV11.HaCaT cells had stable 11 early genes expression. The introducing genome significantly increased proliferation cells, as well proportion in S G2/M phases. After treated with rhIFN-α 2a, IFN signaling pathway was...
Condylomata acuminata (CA), induced by low-risk human papillomaviruses (HPVs), is one of the most common sexually transmitted diseases. The increasing incidence and high recurrence rate CA have significantly contributed to public health problems around world. Because HPVs cannot be cultured in vitro for a long time, there has been little progress development HPV-specific antiviral agents. In this study, we established an HPV11.HaCaT system introducing recircularized genome HPV-11 into HaCaT...
We performed this study to determine the relationship between activation of nuclear factor (NF)-kappaB and inhibition keratinocyte growth by anthralin, which not only might be useful for a better understanding role NF-kappaB in pathogenesis psoriasis, but also indicate whether inflammatory reaction induced anthralin is inseparable from its antipsoriatic activity. The involvement was assessed using drugs leflunomide triptolide (T0) as effectors, since they can inhibit anthralin. results...
Turkey herpesvirus (HVT) vector vaccine expressing hemagglutinin from the H9N2 AIV, namely HVT-H9, were demonstrated to block AIV infection and transmission in chickens. In this study, we evaluated protection efficiency production performance of broilers HVT-H9 field trials presence or absence natural infection. HI titers against harboring maternal antibodies successfully induced by HVT-H9. infection, immunization with blocked reduced mortality rate. Importantly, vaccination slightly...
Condyloma acuminatum, infected by low-risk human papillomaviruses (e.g., HPV6 and HPV11), is one of the most widespread sexually transmitted diseases. Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 proteins (APOBEC3s, A3s) are cellular cytidine deaminases acting as antiviral factors through hypermutation viral genome. However, it remains unknown whether A3s results in HPV11 gene mutations interferon-ω (IFN-ω) exhibits activities system. Here we investigated enhanced...
Objective To investigate the effects of curcumin on mRNA expression cytokines related to Toll-like receptor (TLR) 4 signaling in THP-1 cells.Methods After pretreatment with different concentrations (50,25,12.5 mg/L) or dexamethasone for 12 hours,THP-1 cells were stimulated by lipopolysaccharide (LPS.1 hours followed collection cells.Then total RNA was isolated from these and subjected reverse transcription-polymerase chain reaction (RT-PCR) detection tumor necrosis factor receptor-associated...
Abstract Objective: This study was performed to investigate the effects of honokiol on activation transient receptor potential channel V1 (TRPV1) and secretion thymic stromal lymphopoietin (TSLP) in a human benign epidermal keratinocyte line (HaCaT). Methods: HaCaT keratinocytes were cultivated divided into six groups: capsaicin-induced model control group, capsazepine solvent three treatment groups (7.81, 15.63, 31.25 mg/L honokiol). The effect calcium (Ca 2+ ) influx measured by Ca...
Objective To investigate the significance of different type samples for laboratory detection yellow fever. Methods Different types were collected at time from 5 fever cases, and virus RNA was detected by real-time PCR. Results For cases among in ≥6 days onset, only serum sample 1 patient positive urine other 4 patients positive. Conclusions In early phase fever, viral is a fast sensitive test method, appropriate clinic detection. With lapse time, becomes...
The T-helper typer 17 cells/interleukin (Th17/IL-17) axis,which plays an important role in the pathogenesis of autoimmune diseases,is also implicated development many immune-mediatedskin diseases.Immunosuppressants and immunoregulators have been extensively used treatment inflammatory skin diseases.The exact action mechanisms these drugs are complicated,andrecent studies demonstrated that they may exert immunoregulatory roles by inhibiting Th17/IL-17 axis.Many commonly dermatology,such as...
Objective To investigate the effects of sirolimus and 3-methyladenine (3-MA) on autophagy in,as well as matrix matalloproteinase (MMP)-1 MMP-3 secretion by,human skin fibroblasts (HSFs).Methods HSFs were isolated from circumcised foreskin a healthy male,and subjected to primary culture.After 3 10 passages,HSFs incubated with 20,50,100,250 nmol/L 3-MA 0.5,2.0,5.0,10.0 mmol/L respectively for 4 hours followed by observation detection MMP-1 levels in supernatant enzyme linked immunosorbent...
Objective To evaluate the regulatory effect of serum starvation on autophagy in human HaCaT keratinocytes,and to investigate its molecular mechanism.Methods cells were cultured either Dulbecco's modified Eagle's medium (DMEM) + 10％ fetal calf (control group) or DMEM without (starvation group),for four hours.Then,cell appearance was observed by light microscopy,methyl thiazolyl tetrazolium (MTT) assay performed cell viability,transmission electron microscopy observe ultrastructural...
Objective To observe the changes of autophagy in human skin fibroblast (HSF) model for photoaging. Methods HSF photoaging was established through repeated exposure to ultraviolet B (UVB). Those HSFs receiving no irradiation served as control. The degree aging evaluated by p-galactosidase assay, and level detected. Results After UVB, most pho-toaged were deformed distorted, some them even died. percentage P-galactosidase-positive cells 50.60% ± 5.04% 14.58% 2.69%, respectively photoaged...
Objective To explore if keratinocytes that stably maintain HPV11 genome can be obtained by transfection and selection methods. Methods Escherichia coil containing pBR322.HPV11 plasmid was cultured amplified. Then the extracted, purified digested with BamH Ⅰ enzyme to release viral from bacterial vector. After recovering low-melting point agarose gel electrophoresis, self-circulated T4 DNA ligase. The religated cotransfected pTK-neo into HaCaT using Lipofectamine reagent. G418 for 2 3 weeks,...
[Objective] To observe the effects of autophagy induced by different doses ultraviolet B (UVB) irradiation on apeptosis in human skin fibroblasts.[Methods] Skin fibroblasts were isolated from circumcision specimen a 23-year-old healthy male,and subjected to primary culture.After 3 10 passages,the cells collected and applied following experiment.Methyl thiazolyl tetrazolium (MTT) assay was performed evaluate proliferation some treated with concentrations (0,0.5,2.0,5.0and 10.0 mmol/L)...
Objective To investigate the effects of triptolide on expression a series proteins associated with interferon-γ (IFN-γ)signaling in HaCaT keratinocytes.Methods After pretreatment difrerent dosages triptolide(10-10-10-7 mol/L),HaCaT cells were stimulated by recombinant human IFN-γ(rhIFN-γ,500 U/mL)for various periods followed collection cells.Then,total protein was extracted from these and subjected to Western blotting for detection receptor α(IFN-γRα),phosphorylated Janus kinase 2(pJAK2)and...
Objective To investigate the effects of 13-hexyl-berbefine hydroehlofide (HB-13) and 13-hexyl-paimatine hydrochloride (HP-13) on activation nuclear factor-kappa B (NF-kB) phosphorylation p38 mitogen-activated protein kinase (MAPK) in a human keratinocyte cell line, HaCaT stimulated by tumor necrosis factor alpha (TNF-α). Methods cells were cultured presence various concentrations (0.39, 0.78, 1.56 μg/mL) HB-13 or HP-13 for 120 minutes followed stimulation with recombinant TNF-α (in...